Nucleotide pyrophosphatase/phosphodiesterase 1 exerts a negative effect on starch accumulation and growth in rice seedlings under high temperature and CO₂ concentration conditions

dc.contributor.authorKaneko, Kentaro
dc.contributor.authorInomata, Takuya
dc.contributor.authorMasui, Takahiro
dc.contributor.authorKoshu, Tsutomu
dc.contributor.authorPozueta Romero, Javier
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua, IIM010491.RI1es
dc.date.accessioned2020-09-07T08:46:29Z
dc.date.available2020-09-07T08:46:29Z
dc.date.issued2014
dc.description.abstractNucleotide pyrophosphatase/phosphodiesterase (NPP) is a widely distributed enzymatic activity occurring in both plants and mammals that catalyzes the hydrolytic breakdown of the pyrophosphate and phosphodiester bonds of a number of nucleotides. Unlike mammalian NPPs, the physiological function of plant NPPs remains largely unknown. Using a complete rice NPP1-encoding cDNA as a probe, in this work we have screened a rice shoot cDNA library and obtained complete cDNAs corresponding to six NPP genes (NPP1–NPP6). As a first step to clarify the role of NPPs, recombinant NPP1, NPP2 and NPP6 were purified from transgenic rice cells constitutively expressing NPP1, NPP2 and NPP6, respectively, and their enzymatic properties were characterized. NPP1 and NPP6 exhibited hydrolytic activities toward ATP, UDP-glucose and the starch precursor molecule, ADP-glucose, whereas NPP2 did not recognize nucleotide sugars as substrates, but hydrolyzed UDP, ADP and adenosine 50-phosphosulfate. To gain insight into the physiological function of rice NPP1, an npp1 knockout mutant was characterized. The ADP-glucose hydrolytic activities in shoots of npp1 rice seedlings were 8% of those of the wild type (WT), thus indicating that NPP1 is a major determinant of ADP-glucose hydrolytic activity in rice shoots. Importantly, when seedlings were cultured at 160 Pa CO2 under a 28C/23C (12 h light/12 h dark) regime, npp1 shoots and roots were larger than those of wild-type (WT) seedlings. Furthermore, the starch content in the npp1 shoots was higher than that of WT shoots. Growth and starch accumulation were also enhanced under an atmospheric CO2 concentration (40 Pa) when plants were cultured under a 33C/28C regime. The overall data strongly indicate that NPP1 exerts a negative effect on plant growth and starch accumulation in shoots, especially under high CO2 concentration and high temperature conditions.en
dc.description.sponsorshipThis research was supported by the Ministry of Education, Culture, Sports, Science, and Technology, Japan [Scientific Research on Innovative Areas (22114507) and Grants-in-Aid for Scientific Research (B) (22380186)]; the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) [grant BIO2010-18239]; the Government of Navarra [grant IIM010491.RI1].en
dc.format.extent13 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1093/pcp/pct139
dc.identifier.issn1471-9053
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/38030
dc.language.isoengen
dc.publisherOxford University Pressen
dc.relation.ispartofPlant and Cell Physiology, 2014, 55(2), 320-332en
dc.relation.publisherversionhttps://doi.org/10.1093/pcp/pct139
dc.rights© The Author 2013. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subjectADP-glucoseen
dc.subjectCO₂en
dc.subjectNPPen
dc.subjectOryza sativaen
dc.subjectPlastiden
dc.subjectStarchen
dc.titleNucleotide pyrophosphatase/phosphodiesterase 1 exerts a negative effect on starch accumulation and growth in rice seedlings under high temperature and CO₂ concentration conditionsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication80c8bd69-4127-4474-a679-3b10747cae42
relation.isAuthorOfPublication.latestForDiscovery80c8bd69-4127-4474-a679-3b10747cae42

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
04_Kaneko_NucleotidePyrophosphatase.pdf
Size:
860.16 KB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description: