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Regulation of heterogenous lexA expression in staphylococcus aureus by an antisense RNA originating from transcriptional read-through upon natural mispairings in the sbrB intrinsic terminator

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dc.contributor.authorBastet, Laurène
dc.contributor.authorBustos-Sanmamed, Pilar
dc.contributor.authorCatalán Moreno, Arancha
dc.contributor.authorCaballero Sánchez, Carlos
dc.contributor.authorCuesta Ferre, Sergio
dc.contributor.authorMatilla Cuenca, Leticia
dc.contributor.authorVillanueva San Martín, Maite
dc.contributor.authorValle Turrillas, Jaione
dc.contributor.authorLasa Uzcudun, Íñigo
dc.contributor.authorToledo Arana, Alejandro
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2022-04-12T06:27:11Z
dc.date.available2022-04-12T06:27:11Z
dc.date.issued2022
dc.description.abstractBacterial genomes are pervasively transcribed, generating a wide variety of antisense RNAs (asRNAs). Many of them originate from transcriptional read-through events (TREs) during the transcription termination process. Previous transcriptome analyses revealed that the lexA gene from Staphylococcus aureus, which encodes the main SOS response regulator, is affected by the presence of an asRNA. Here, we show that the lexA antisense RNA (lexA-asRNA) is generated by a TRE on the intrinsic terminator (TTsbrB) of the sbrB gene, which is located downstream of lexA, in the opposite strand. Transcriptional read-through occurs by a natural mutation that destabilizes the TTsbrB structure and modifies the efficiency of the intrinsic terminator. Restoring the mispairing mutation in the hairpin of TTsbrB prevented lexA-asRNA transcription. The level of lexA-asRNA directly correlated with cellular stress since the expressions of sbrB and lexA-asRNA depend on the stress transcription factor SigB. Comparative analyses revealed strain-specific nucleotide polymorphisms within TTsbrB, suggesting that this TT could be prone to accumulating natural mutations. A genome-wide analysis of TREs suggested that mispairings in TT hairpins might provide wider transcriptional connections with downstream genes and, ultimately, transcriptomic variability among S. aureus strains.en
dc.description.sponsorshipThis work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant no. 646869 to A.T.-A.) and by the Spanish Ministry of Science and Innovation grants (BIO2017-83035-R to I.L. and PID2019-105216GB-I00 to A.T.-A.). Funding for open access charge was provided by the CSIC Open Access Publication Support Initiative, Unit of Information Resources for Research (URICI).en
dc.format.extent24 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.3390/ijms23010576
dc.identifier.issn1661-6596
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/42724
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofInternational Journal of Molecular Sciences, 23 (1), 2022en
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/646869en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83035-R/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105216GB-I00/ES/en
dc.relation.publisherversionhttps://doi.org/10.3390/ijms23010576
dc.rights© 2022 by the authors. Creative Commons Attribution 4.0 Internationalen
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAntisense RNAen
dc.subjectLexAen
dc.subjectPosttranscriptional regulationen
dc.subjectStaphylococcus aureusen
dc.subjectTranscriptional read-throughen
dc.subjectTranscriptional terminationen
dc.titleRegulation of heterogenous lexA expression in staphylococcus aureus by an antisense RNA originating from transcriptional read-through upon natural mispairings in the sbrB intrinsic terminatoren
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dspace.entity.typePublication
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relation.isAuthorOfPublication15dfff18-cd2b-465a-b247-b583e5fffa3c
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relation.isAuthorOfPublicationc654d104-1ae2-41cf-9215-4b4bed3e5ea6
relation.isAuthorOfPublication777af947-83d1-4cb7-b485-b0430d3e28be
relation.isAuthorOfPublication.latestForDiscoveryec39ab9f-6762-4848-8613-8a381983f9f8

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