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Comparative genomic and phylogenetic analyses of gammaproteobacterial glg genes traced the origin of the Escherichia coli glycogen glgBXCAP operon to the last common ancestor of the sister orders enterobacteriales and pasteurellales

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dc.contributor.authorAlmagro Zabalza, Goizeder
dc.contributor.authorViale, Alejandro M.
dc.contributor.authorMontero Macarro, Manuel
dc.contributor.authorRahimpour, Mehdies_ES
dc.contributor.authorMuñoz Pérez, Francisco José
dc.contributor.authorBaroja Fernández, Edurne
dc.contributor.authorBahaji, Abdellatif
dc.contributor.authorZúñiga, Manueles_ES
dc.contributor.authorGonzález Candelas, Fernandoes_ES
dc.contributor.authorPozueta Romero, Javier
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2018-09-06T12:20:17Z
dc.date.available2018-09-06T12:20:17Z
dc.date.issued2015
dc.description.abstractProduction of branched α-glucan, glycogen-like polymers is widely spread in the Bacteria domain. The glycogen pathway of synthesis and degradation has been fairly well characterized in the model enterobacterial species Escherichia coli (order Enterobacteriales, class Gammaproteobacteria), in which the cognate genes (branching enzyme glgB, debranching enzyme glgX, ADP-glucose pyrophosphorylase glgC, glycogen synthase glgA, and glycogen phosphorylase glgP) are clustered in a glgBXCAP operon arrangement. However, the evolutionary origin of this particular arrangement and of its constituent genes is unknown. Here, by using 265 complete gammaproteobacterial genomes we have carried out a comparative analysis of the presence, copy number and arrangement of glg genes in all lineages of the Gammaproteobacteria. These analyses revealed large variations in glg gene presence, copy number and arrangements among different gammaproteobacterial lineages. However, the glgBXCAP arrangement was remarkably conserved in all glg-possessing species of the orders Enterobacteriales and Pasteurellales (the E/P group). Subsequent phylogenetic analyses of glg genes present in the Gammaproteobacteria and in other main bacterial groups indicated that glg genes have undergone a complex evolutionary history in which horizontal gene transfer may have played an important role. These analyses also revealed that the E/P glgBXCAP genes (a) share a common evolutionary origin, (b) were vertically transmitted within the E/P group, and (c) are closely related to glg genes of some phylogenetically distant betaproteobacterial species. The overall data allowed tracing the origin of the E. coli glgBXCAP operon to the last common ancestor of the E/P group, and also to uncover a likely glgBXCAP transfer event from the E/P group to particular lineages of the Betaproteobacteria.en
dc.description.sponsorshipThis research was partially supported by the grant [BIO2010-18239] from the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain). MR acknowledges a pre-doctoral JAE fellowship from the Consejo Superior de Investigaciones Científicas. AMV is grateful to the funding of the Programa Campus Iberus de Excelencia Internacional, Ministerio de Educación, Spain. His 2-month visit (January-March 2014) to the Institute of Agrobiotechnology, Public Universtity of Navarra, Pamplona, Spain, was included into the Proyecto financiado por el Ministerio de Educación en el marco del Programa Campus de Excelencia Internacional. FGC was supported by project [BFU2011-24112] from the Ministerio de Ciencia e Innovación (Spain).en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1371/journal.pone.0115516
dc.identifier.issn1932-6203
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/30539
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPlos One, 10(1): e0115516en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/BFU2011-24112en
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0115516
dc.rights© 2015 Almagro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGammaproteobacteriaen
dc.subjectglgBXCAP operonen
dc.subjectEscherichia colien
dc.subjectglg genesen
dc.titleComparative genomic and phylogenetic analyses of gammaproteobacterial glg genes traced the origin of the Escherichia coli glycogen glgBXCAP operon to the last common ancestor of the sister orders enterobacteriales and pasteurellalesen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
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