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The extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice

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dc.contributor.authorSan Román Aberasturi, Beatriz
dc.contributor.authorLasa Uzcudun, Íñigo
dc.contributor.authorAndrés Cara, Damián de
dc.contributor.authorAmorena Zabalza, Beatriz
dc.contributor.authorLasarte, Juan José
dc.contributor.authorGrilló Dolset, María Jesús
dc.contributor.authorGarrido González, Victoria
dc.contributor.authorMuñoz Álvaro, Pilar María
dc.contributor.authorArribillaga, Laura
dc.contributor.authorGarcía Martínez, Begoña
dc.contributor.authorAndrés, Ximena de
dc.contributor.authorZabaleta Sanz de Acedo, Virginia
dc.contributor.authorMansilla, Cristina
dc.contributor.authorFarrán Blanch, Inmaculada
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua: IIM10865.RI1-EP12es
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2014-06-20T06:37:09Z
dc.date.available2014-06-20T06:37:09Z
dc.date.issued2012
dc.description.abstractThe Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidis). Since lipopolysaccharide (LPS) is a major virulence factor and the LPS O-polysaccharide (O-PS) is the immunodominant antigen in serological diagnostic tests, Salmonella mutants lacking O-PS (rough mutants) represent an interesting approach for developing new vaccines and diagnostic tests to differentiate infected and vaccinated animals (DIVA tests). Here, antigenic preparations (hot-saline extracts and formalin-inactivated bacterins) from two Salmonella Enteritidis rough mutants, carrying either intact (SE Delta waaL) or deep-defective (SE Delta gal) LPS-Core, were used in combination with EDA. Biotinylated bacterins, in particular SE Delta waaL bacterin, decorated with EDAvidin (EDA and streptavidin fusion protein) improved the protection conferred by hot-saline or bacterins alone and prevented significantly the virulent infection at least to the levels of live attenuated rough mutants. These findings demonstrate the adjuvant effect of EDAvidin when administered with biotinylated bacterins from Salmonella Enteritidis lacking O-PS and the usefulness of BEDA-SE Delta waaL as non-live vaccine in the mouse model.en
dc.description.sponsorshipThis work was funded by Gobierno de Navarra and European Union (project EuroInnova-Navarra reference IIM10865.RI1-EP12). B.S.R., P.M.M. and X.D.A. post-doctoral contracts were granted by Gobierno de Navarra/JAE-doc CSIC, MICINN (Subprograma Juan de la Cierva) and Universidad Pública de Navarra, respectively.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1186/1297-9716-43-31
dc.identifier.issn1297-9716 (electronic)
dc.identifier.issn0928-4249 (print)
dc.identifier.other1277
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/10925
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.ispartofVeterinary Research, 2012, 43:31en
dc.relation.publisherversionhttps://doi.org/10.1186/1297-9716-43-31
dc.rights© 2012 San Román et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.subjectBiofilm formationen
dc.subjectEnteritidisen
dc.subjectVeterinary sciencesen
dc.subjectMutantsen
dc.subjectProteinen
dc.subjectInfectionen
dc.subjectBrucella ovisen
dc.subjectExtra domain-aen
dc.subjectProtective immunityen
dc.subjectEnterica serovar typhimuriumen
dc.subjectPhage-typing schemeen
dc.titleThe extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in miceen
dc.typeinfo:eu-repo/semantics/article
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dspace.entity.typePublication
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