Short-term exposure to high atmospheric vapor pressure deficit (Vpd) severely impacts durum wheat carbon and nitrogen metabolism in the absence of edaphic water stress

Fakhet, Dorra; Morales Iribas, Fermín; Jáuregui Mosquera, Iván; Erice, G.; Aparicio Tejo, Pedro María; González Murua, Carmen; Aranjuelo Michelena, Iker; Aroca, Ricardo; Irigoyen, Juan J.

Short-term exposure to high atmospheric vapor pressure deficit (Vpd) severely impacts durum wheat carbon and nitrogen metabolism in the absence of edaphic water stress

dc.contributor.author	Fakhet, Dorra
dc.contributor.author	Morales Iribas, Fermín
dc.contributor.author	Jáuregui Mosquera, Iván
dc.contributor.author	Erice, G.
dc.contributor.author	Aparicio Tejo, Pedro María
dc.contributor.author	González Murua, Carmen
dc.contributor.author	Aranjuelo Michelena, Iker
dc.contributor.author	Aroca, Ricardo
dc.contributor.author	Irigoyen, Juan J.
dc.contributor.department	Ciencias	es_ES
dc.contributor.department	Zientziak	eu
dc.date.accessioned	2021-06-16T08:48:56Z
dc.date.available	2021-06-16T08:48:56Z
dc.date.issued	2021
dc.description	Incluye material complementario	es_ES
dc.description.abstract	Low atmospheric relative humidity (RH) accompanied by elevated air temperature and decreased precipitation are environmental challenges that wheat production will face in future decades. These changes to the atmosphere are causing increases in air vapor pressure deficit (VPD) and low soil water availability during certain periods of the wheat-growing season. The main objective of this study was to analyze the physiological, metabolic, and transcriptional response of carbon (C) and nitrogen (N) metabolism of wheat (Triticum durum cv. Sula) to increases in VPD and soil water stress conditions, either alone or in combination. Plants were first grown in well-watered conditions and near-ambient temperature and RH in temperature-gradient greenhouses until anthesis, and they were then subjected to two different water regimes well-watered (WW) and water-stressed (WS), i.e., watered at 50% of the control for one week, followed by two VPD levels (low, 1.01/0.36 KPa and high, 2.27/0.62 KPa; day/night) for five additional days. Both VPD and soil water content had an important impact on water status and the plant physiological apparatus. While high VPD and water stress-induced stomatal closure affected photosynthetic rates, in the case of plants watered at 50%, high VPD also caused a direct impairment of the RuBisCO large subunit, RuBisCO activase and the electron transport rate. Regarding N metabolism, the gene expression, nitrite reductase (NIR) and transport levels detected in young leaves, as well as determinations of the δ15N and amino acid profiles (arginine, leucine, tryptophan, aspartic acid, and serine) indicated activation of N metabolism and final transport of nitrate to leaves and photosynthesizing cells. On the other hand, under low VPD conditions, a positive effect was only observed on gene expression related to the final step of nitrate supply to photosynthesizing cells, whereas the amount of15N supplied to the roots that reached the leaves decreased. Such an effect would suggest an impaired N remobilization from other organs to young leaves under water stress conditions and low VPD.	en
dc.description.sponsorship	This work was supported by the Spanish Innovation and Universities Ministry (AGL2016-79868-R; 427 PCIN-2017-007) and the Basque Country Government consolidated group program (IT-932-16).	en
dc.format.extent	16 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.3390/plants10010120
dc.identifier.issn	2223-7747
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/39934
dc.language.iso	eng	en
dc.publisher	MDPI	en
dc.relation.ispartof	Plants, 2021, 10, 120	en
dc.relation.projectID	info:eu-repo/grantAgreement/ES/1PE/AGL2016-79868-R/
dc.relation.publisherversion	https://doi.org/10.3390/plants10010120
dc.rights	© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	C/N metabolism	en
dc.subject	Drought	en
dc.subject	Durum wheat	en
dc.subject	Physiology	en
dc.subject	Relative humidity	en
dc.subject	Vapor pressure deficit	en
dc.title	Short-term exposure to high atmospheric vapor pressure deficit (Vpd) severely impacts durum wheat carbon and nitrogen metabolism in the absence of edaphic water stress	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
relation.isAuthorOfPublication	ebca79b9-1e2a-4729-b4ce-295c81474eb4
relation.isAuthorOfPublication	19699fdc-1098-4a1c-b547-7849e8634c2a
relation.isAuthorOfPublication	b8dd84ae-83ed-4e3f-873e-b0023505b3df
relation.isAuthorOfPublication.latestForDiscovery	ebca79b9-1e2a-4729-b4ce-295c81474eb4