Killing niche competitors by remote-control bacteriophage induction

Selva, Laura; Viana, David; Regev Yochay, Gili; Trzcinski, Krzysztof; Corpa, Juan Manuel; Lasa Uzcudun, Íñigo; Novick, Richard P.; Penadés, José R.

Publication:
Killing niche competitors by remote-control bacteriophage induction

dc.contributor.author	Selva, Laura
dc.contributor.author	Viana, David
dc.contributor.author	Regev Yochay, Gili
dc.contributor.author	Trzcinski, Krzysztof
dc.contributor.author	Corpa, Juan Manuel
dc.contributor.author	Lasa Uzcudun, Íñigo
dc.contributor.author	Novick, Richard P.
dc.contributor.author	Penadés, José R.
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2018-12-28T09:14:03Z
dc.date.available	2018-12-28T09:14:03Z
dc.date.issued	2009
dc.description.abstract	A surprising example of interspecies competition is the production by certain bacteria of hydrogen peroxide at concentrations that are lethal for others. A case in point is the displacement of Staphylococcus aureus by Streptococcus pneumoniae in the nasopharynx, which is of considerable clinical significance. How it is accomplished, however, has been a great mystery, because H2O2 is a very well known disinfectant whose lethality is largely due to the production of hyperoxides through the abiological Fenton reaction. In this report, we have solved the mystery by showing that H2O2 at the concentrations typically produced by pneumococci kills lysogenic but not nonlysogenic staphylococci by inducing the SOS response. The SOS response, a stress response to DNA damage, not only invokes DNA repair mechanisms but also induces resident prophages, and the resulting lysis is responsible for H2O2 lethality. Because the vast majority of S. aureus strains are lysogenic, the production of H2O2 is a very widely effective antistaphylococcal strategy. Pneumococci, however, which are also commonly lysogenic and undergo SOS induction in response to DNA-damaging agents such as mitomycin C, are not SOS-induced on exposure to H2O2. This is apparently because they are resistant to the DNAdamaging effects of the Fenton reaction. The production of an SOS-inducing signal to activate prophages in neighboring organisms is thus a rather unique competitive strategy, which we suggest may be in widespread use for bacterial interference. However, this strategy has as a by-product the release of active phage, which can potentially spread mobile genetic elements carrying virulence genes.	en
dc.description.sponsorship	This work was supported by Comisión Interministerial de Ciencia y Tecnología Grants BIO2005-08399-C02-02, BIO2008-05284-C02-02, and BIO2008-00642-E/C; Cardenal Herrera-CEU University Grants PRCEUUCH25/ 08 and Copernicus program; and by Conselleria de Agricultura, Pesca i Alimentació (CAPiA), and from the Generalitat Valenciana (ACOMP07/258) (J.R.P.). L.S. and D.V. were supported by Cardenal Herrera-CEU University fellowships.	en
dc.format.extent	5 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1073/pnas.0809600106
dc.identifier.issn	0027-8424 (Print)
dc.identifier.issn	1091-6490 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/31883
dc.language.iso	eng	en
dc.publisher	National Academy of Sciences	en
dc.relation.ispartof	PNAS, January 27, 2009 106 (4) 1234-1238	en
dc.relation.publisherversion	https://doi.org/10.1073/pnas.0809600106
dc.rights	© 2009 by The National Academy of Sciences of the USA	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.subject	Hydrogen peroxide	en
dc.subject	SOS response	en
dc.subject	Staphylococcus aureus	en
dc.subject	Streptococcus pneumoniae	en
dc.subject	Bacterial interference	en
dc.title	Killing niche competitors by remote-control bacteriophage induction	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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relation.isAuthorOfPublication.latestForDiscovery	c654d104-1ae2-41cf-9215-4b4bed3e5ea6