Publication: Overlapping transcription and bacterial RNA removal
| dc.contributor.author | Lasa Uzcudun, Íñigo | |
| dc.contributor.author | Villanueva San Martín, Maite | |
| dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es_ES |
| dc.date.accessioned | 2016-11-04T16:26:15Z | |
| dc.date.available | 2016-11-04T16:26:15Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | The precise understanding of the biology of a living cell requires the identification and quantification of the molecular components necessary to sustain life. One such element is RNA. Two independent high-throughput strategies are available to identify the entire collection of RNA molecules produced by a cell population, which is currently known as the transcriptome. One technique relies on microarray technology (tiling arrays), whereas the second one relies on sequencing the RNA pool (RNA-seq) (1). Both techniques offer the advantage that the identification of the RNA content is not biased by protein-based genome annotation. The application of these methods to the transcriptome analysis in bacteria has uncovered the existence of a large amount of RNA molecules that overlap at least in some portion with protein-encoding RNA transcripts, generating perfect sense/antisense RNA duplexes (2⇓⇓–5). However, because transcriptome studies have been performed using microgram amounts of RNA purified from millions of bacterial cells instead of RNA purified from a single bacterium, the presence of overlapping sense/antisense RNAs from a genomic region does not necessarily mean that both sense and antisense transcripts are simultaneously present in the same bacteria. Hence, it might be possible that a subgroup in the bacterial population synthesized the sense transcript, another subgroup synthesized the antisense transcript, and consequently overlapping transcripts would never be together in the same cell. A report in PNAS by Lybecker et al. (6) provides clear evidences that both sense and antisense transcripts can be present simultaneously within the same bacterial cell. Using a monoclonal antibody that recognizes double-stranded RNA molecules (dsRNA) irrespectively of the nucleotide sequence, the authors perform immunoprecipitation assays to pull down dsRNA molecules (IP-dsRNA) from a total RNA sample extracted from Escherichia coli, and identified the purified dsRNA by RNA-seq. | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1073/pnas.1324236111 | |
| dc.identifier.issn | 1091-6490 (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/22615 | |
| dc.language.iso | eng | en |
| dc.publisher | National Academy of Sciences | en |
| dc.relation.ispartof | PNAS, February 25, 2014, vol. 111 no. 8 | en |
| dc.relation.publisherversion | https://dx.doi.org/10.1073/pnas.1324236111 | |
| dc.rights | © National Academy of Sciences | en |
| dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
| dc.title | Overlapping transcription and bacterial RNA removal | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | Versión aceptada / Onetsi den bertsioa | es |
| dc.type.version | info:eu-repo/semantics/acceptedVersion | en |
| dspace.entity.type | Publication | |
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