Ras module function is involved in regulation of sexual development Schizophyllum commune
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
The white rot fungus Schizophyllum commune is used as a model to investigate
sexual development in hymenomycetes. We isolated the gene gap1 encoding
a GTPase-activating protein for Ras. Disruption of gap1 should
therefore lead to strains accumulating Ras in its activated, GTP-bound state
and to constitutive Ras signaling. Mating behavior was not altered in Δgap1
monokaryons whereas growth rate in ...
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The white rot fungus Schizophyllum commune is used as a model to investigate
sexual development in hymenomycetes. We isolated the gene gap1 encoding
a GTPase-activating protein for Ras. Disruption of gap1 should
therefore lead to strains accumulating Ras in its activated, GTP-bound state
and to constitutive Ras signaling. Mating behavior was not altered in Δgap1
monokaryons whereas growth rate in Δgap1 monokaryons was reduced about
25%. Dikaryotic Δgap1/Δgap1 strains displayed 50% growth reduction. Hyphal
growth was disturbed showing a wavy growth pattern. In dikaryons,
clamp formation was severly disturbed as hook cells failed to fuse with the
penultimate cell at the site that in wildtype cells is marked by a peg formed
from the mother cell. Instead, the dikaryotic character of the hyphae was rescued
by fusion of the hooks with nearby developing branches. The mating
type genes of the B factors encoding a pheromone receptor system are known
to be required for clamp cell fusion. A role for Ras in the same process in discussed.
Fruitbody formation was observed in homozygous Δgap1/Δgap1
dikaryons which, however, formed increased numbers of fruit body primordia,
whereas the amount of fruit bodies was not raised. Mature fruit bodies
formed no or abnormal gills. No production of spores could be observed.
Similar phenotypes in fruitbody development had been previously described
for elevated intracellular cAMP levels. Thus, the signalling of Ras is discussed
with respect to cAMP signalling. [--]
Materias
Schizophyllum commune,
Ras module function
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.