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Staphylococcus aureus pathogenicity island DNA is packaged in particles composed of phage proteins
Staphylococcus aureus pathogenicity islands (SaPIs) have an intimate relationship with temperate staphylococcal phages. During phage growth, SaPIs are induced to replicate and are efficiently encapsidated into special small ... -
Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep
Background: A central nervous system (CNS) disease outbreak caused by small ruminant lentiviruses (SRLV) has triggered interest in Spain due to the rapid onset of clinical signs and relevant production losses. In a previous ... -
Sucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production
Sucrose synthase (SUS) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate-glucose and fructose. In Arabidopsis, a multigene family encodes six SUS ... -
A super-family of transcriptional activators regulates bacteriophage packaging and lysis in Gram-positive bacteria
The propagation of bacteriophages and other mobile genetic elements requires exploitation of the phage mechanisms involved in virion assembly and DNA packaging. Here, we identified and characterized four different families ... -
Superinfection exclusion in alphabaculovirus infections is concomitant with actin reorganization
Superinfection exclusion is the ability of an established virus to interfere with a second virus infection. This effect was studied in vitro during lepidopteran-specific nucleopolyhedrovirus (genus Alphabaculovirus, family ... -
Systematic production of inactivating and non-inactivating suppressor mutations at the relA locus that compensate the detrimental effects of complete spoT loss and affect glycogen content in Escherichia coli
In Escherichia coli, ppGpp is a major determinant of growth and glycogen accumulation. Levels of this signaling nucleotide are controlled by the balanced activities of the ppGpp RelA synthetase and the dual-function ... -
Tobacco plastidial thioredoxins as modulators of recombinant protein production in transgenic chloroplasts
Thioredoxins (Trxs) are small ubiquitous disulphide proteins widely known to enhance expression and solubility of recombinant proteins in microbial expression systems. Given the common evolutionary heritage of chloroplasts ... -
Towards the identification of the common features of bacterial biofilm development
Los microorganismos pueden vivir y proliferar como células individuales que nadan libremente en el medio o crecer en comunidades multicelulares muy bien organizadas dentro de una matriz que ellas mismas han sintetizado y ... -
Transformed recombinant enrichment profiling rapidly identifies HMW1 as an intracellular invasion locus in Haemophilus influenzae
Many bacterial species actively take up and recombine homologous DNA into their genomes, called natural competence, a trait that offers a means to identify the genetic basis of naturally occurring phenotypic variation. ... -
Unraveling the role of transient starch in the response of Arabidopsis to elevated CO2 under long-day conditions
Previous studies on Arabidopsis under long-term exposure to elevated CO2 have been conducted using starch synthesis and breakdown mutants cultured under short day conditions. These studies showed that starch synthesis can ... -
Unravelling the mechanisms that improve photosynthetic performance of N₂-fixing pea plants exposed to elevated [CO₂]
Although the predicted enhanced photosynthetic rates of plants exposed to elevated [CO₂] are expected to increase carbohydrate and plant growth, recent findings have shown a complex regulation of these processes. The aim ... -
The Vip3Ag4 insecticidal protoxin from bacillus thuringiensis adopts a tetrameric configuration that is maintained on proteolysis
The Vip3 proteins produced during vegetative growth by strains of the bacterium Bacillus thuringiensis show insecticidal activity against lepidopteran insects with a mechanism of action that may involve pore formation and ... -
Vip3C, a novel class of vegetative insecticidal proteins from Bacillus thuringiensis
Three vip3 genes were identified in two Bacillus thuringiensis Spanish collections. Sequence analysis revealed a novel Vip3 protein class (Vip3C). Preliminary bioassays of larvae from 10 different lepidopteran species ... -
Viral load, tissue distribution and histopathological lesions in goats naturally and experimentally infected with the Small Ruminant Lentivirus Genotype E (subtype E1 Roccaverano strain)
Small Ruminant Lentivirus (SRLV) subtype E1, also known as Roccaverano strain, is considered a low pathogenic virus on the basis of natural genetic deletions, in vitro properties and on-farm observations. In order to gain ... -
Volatile compounds emitted by diverse phytopathogenic microorganisms promote plant growth and flowering through cytokinin action
It is known that volatile emissions from some beneficial rhizosphere microorganisms promote plant growth. Here we show that volatile compounds (VCs) emitted by phylogenetically diverse rhizosphere and non-rhizhosphere ... -
Wavelet-based detection of transcriptional activity on a novel Staphylococcus aureus tiling microarray
Background: High-density oligonucleotide microarray is an appropriate technology for genomic analysis, and is particulary useful in the generation of transcriptional maps, ChIP-on-chip studies and re-sequencing of the ... -
What have we learned from brucellosis in the mouse model?
Brucellosis is a zoonosis caused by Brucella species. Brucellosis research in natural hosts is often precluded by practical, economical and ethical reasons and mice are widely used. However, mice are not natural Brucella ... -
Yield and quality of sugar snap pea in the Ebro Valley: sowing date and seed density
(Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 2012) Artículo / ArtikuluaSugar snap pea (Pisum sativum L. var. macrocarpon Ser.) is an edible-podded sweet pea that is being considered as a new totally mechanized crop to supply raw material to the agri-food industry of the Ebro Valley (Northern ...