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dc.creatorBaslam, Marouanees_ES
dc.creatorBaroja Fernández, Edurnees_ES
dc.creatorRicarte Bermejo, Adrianaes_ES
dc.creatorSánchez López, Ángela Maríaes_ES
dc.creatorAranjuelo Michelena, Ikeres_ES
dc.creatorBahaji, Abdellatifes_ES
dc.creatorMuñoz Pérez, Francisco Josées_ES
dc.creatorAlmagro Zabalza, Goizederes_ES
dc.creatorPujol, Pabloes_ES
dc.creatorGalarza, Reginaes_ES
dc.creatorTeixidor, Pilares_ES
dc.creatorPozueta Romero, Javieres_ES
dc.date.accessioned2018-09-06T12:20:19Z
dc.date.available2018-09-06T12:20:19Z
dc.date.issued2017
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2454/30546
dc.description.abstractAlthough there is a great wealth of data supporting the occurrence of simultaneous synthesis and breakdown of storage carbohydrate in many organisms, previous 13CO2 pulse-chase based studies indicated that starch degradation does not operate in illuminated Arabidopsis leaves. Here we show that leaves of gwd, sex4, bam4, bam1/bam3 and amy3/isa3/lda starch breakdown mutants accumulate higher levels of starch than wild type (WT) leaves when cultured under continuous light (CL) conditions. We also show that leaves of CL grown dpe1 plants impaired in the plastidic disproportionating enzyme accumulate higher levels of maltotriose than WT leaves, the overall data providing evidence for the occurrence of extensive starch degradation in illuminated leaves. Moreover, we show that leaves of CL grown mex1/ pglct plants impaired in the chloroplastic maltose and glucose transporters display a severe dwarf phenotype and accumulate high levels of maltose, strongly indicating that the MEX1 and pGlcT transporters are involved in the export of starch breakdown products to the cytosol to support growth during illumination. To investigate whether starch breakdown products can be recycled back to starch during illumination through a mechanism involving ADP-glucose pyrophosphorylase (AGP) we conducted kinetic analyses of the stable isotope carbon composition (δ13C) in starch of leaves of 13CO2 pulsed-chased WT and AGP lacking aps1 plants. Notably, the rate of increase of δ13C in starch of aps1 leaves during the pulse was exceedingly higher than that of WT leaves. Furthermore, δ13C decline in starch of aps1 leaves during the chase was much faster than that of WT leaves, which provides strong evidence for the occurrence of AGP-mediated cycling of starch breakdown products in illuminated Arabidopsis leaves.en
dc.description.sponsorshipComisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) Grant numbers: BIO2010-18239 and BIO2013-49125-C2-1-P.en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPlos One, 12(2): e0171245.en
dc.rights© 2017 Baslam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectArabidopsis leavesen
dc.subjectStarchen
dc.titleGenetic and isotope ratio mass spectrometric evidence for the occurrence of starch degradation and cycling in illuminated Arabidopsis leavesen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1371/journal.pone.0171245
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/BIO2013-49125en
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0171245
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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© 2017 Baslam et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License, which
permits unrestricted use, distribution, and
reproduction in any medium, provided the original
author and source are credited.
Except where otherwise noted, this item's license is described as © 2017 Baslam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.