• Login
    View Item 
    •   Academica-e
    • Departamentos y Centros - Sailak eta Ikastegiak
    • IdAB / Instituto de Agrobiotecnología - Agrobioteknologiako Institutua
    • Artículos de revista IdAB - IdAB Aldizkari artikuluak
    • View Item
    •   Academica-e
    • Departamentos y Centros - Sailak eta Ikastegiak
    • IdAB / Instituto de Agrobiotecnología - Agrobioteknologiako Institutua
    • Artículos de revista IdAB - IdAB Aldizkari artikuluak
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Overexpression of the recA gene decreases oral but not intraperitoneal fitness of Salmonella enterica

    Thumbnail
    View/Open
    13_Latasa_Overexpression.pdf (1.562Mb)
    Date
    2010
    Author
    Medina-Ruiz, Laura 
    Campoy Sánchez, Susana 
    Latasa Osta, Cristina Upna
    Cardenas, Paula 
    Alonso, Juan Carlos 
    Barbé, Jordi 
    Version
    Acceso abierto / Sarbide irekia
    Type
    Artículo / Artikulua
    Version
    Versión publicada / Argitaratu den bertsioa
    Impact
     
     
     
    10.1128/iai.01321-09
     
     
     
    Show full item record
    Abstract
    Transcription of the Salmonella enterica recA gene is negatively controlled by the LexA protein, the repressor of the SOS response. The introduction of a mutation (recAo6869) in the LexA binding site, in the promoter region of the S. enterica ATCC 14028 recA gene, allowed the analysis of the effect that RecA protein overproduction has on the fitness of this virulent strain. The fitness of orally ... [++]
    Transcription of the Salmonella enterica recA gene is negatively controlled by the LexA protein, the repressor of the SOS response. The introduction of a mutation (recAo6869) in the LexA binding site, in the promoter region of the S. enterica ATCC 14028 recA gene, allowed the analysis of the effect that RecA protein overproduction has on the fitness of this virulent strain. The fitness of orally but not intraperitoneally inoculated recAo6869 cells decreased dramatically. However, the SOS response of this mutant was induced normally, and there was no increase in the sensitivity of the strain toward DNA-damaging agents, bile salts, or alterations in pH. Nevertheless, S. enterica recAo6869 cells were unable to swarm and their capacity to cross the intestinal epithelium was significantly reduced. The swarming deficiency in recAo6869 cells is independent of the flagellar phase. Moreover, swimming activity of the recAo6869 strain was not diminished with respect to the wild type, indicating that the flagellar synthesis is not affected by RecA protein overproduction. In contrast, swarming was recovered in a recAo6869 derivative that overproduced CheW, a protein known to be essential for this function. These data demonstrate that an equilibrium between the intracellular concentrations of RecA and CheW is necessary for swarming in S. enterica. Our results are the first to point out that the SOS response plays a critical role in the prevention of DNA damage by abolishing bacterial swarming in the presence of a genotoxic compound. [--]
    Subject
    Salmonella enterica, Rec A
     
    Publisher
    American Society for Microbiology
    Published in
    Infection and immunity, vol. 78, nº 7, july 2010, p. 3217–3225
    Departament
    IdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    Publisher version
    https://doi.org/10.1128/iai.01321-09
    URI
    https://hdl.handle.net/2454/32081
    Sponsorship
    This work was funded by grants BFU2008-01078 from the Ministerio de Ciencia e Innovación (MICINN) de España and 2009SGR1106 from the Generalitat de Catalunya to J.B. and BFU2009-07167 from MICINN to J.C.A. Laura Medina was the recipient of a predoctoral fellowship from the MICINN.
    Appears in Collections
    • Artículos de revista IdAB - IdAB Aldizkari artikuluak [189]
    • Artículos de revista - Aldizkari artikuluak [4586]
    Items in Academica-e are protected by copyright with all rights reserved, unless otherwise noted.

     © Universidad Pública de Navarra - Nafarroako Unibertsitate Publikoa
         Repositorio basado en DSpace

    Contact Us | Send Feedback
     

     

    Browse

    All of Academica-eCommunities & CollectionsAuthorsAuthors By Issue DateTitlesSubjectsBy DegreeThis CollectionAuthorsAuthors By Issue DateTitlesSubjectsBy Degree

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

     © Universidad Pública de Navarra - Nafarroako Unibertsitate Publikoa
         Repositorio basado en DSpace

    Contact Us | Send Feedback