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dc.creatorRotinen Díaz, Mirja Sofiaes_ES
dc.creatorVillar Bécares, Joaquínes_ES
dc.creatorCelay Leoz, Iones_ES
dc.creatorSerrano Mendioroz, Irantzues_ES
dc.creatorNotario, Vicentees_ES
dc.creatorEncío Martínez, Ignacioes_ES
dc.identifier.issn0303-7207 (Print)
dc.identifier.issn1872-8057 (Electronic)
dc.description.abstractType 11 Hydroxysteroid (17-beta) dehydrogenase (HSD17B11) catalyzes the conversion of 5α-androstan-3α,17β-diol into androsterone suggesting that it may play an important role in androgen metabolism. We previously described that overexpression of C/EBPα or C/EBPβ induced HSD17B11 expression in HepG2 cells but this process was not mediated by the CCAAT boxes located within its proximal promoter region. Here, we study HSD17B11 transcriptional regulation in prostate cancer (PC) cells. Transfection experiments showed that the region −107/+18 is sufficient for promoter activity in PC cells. Mutagenesis analysis indicated that Sp1 and C/EBP binding sites found in this region are essential for promoter activity. Additional experiments demonstrated that ectopic expression of Sp1 and C/EBPα upregulated HSD17B11 expression only in PC cell lines. Through DAPA and ChIP assays, specific recruitment of Sp1 and C/EBPα to the HSD17B11 promoter was detected. These results show that HSD17B11 transcription in PC cells is regulated by Sp1 and C/EBPα.en
dc.description.sponsorshipM. R. and J.C. were recipients of a fellowship from the Departamento de Educación, Gobierno de Navarra.en
dc.format.extent17 p.
dc.relation.ispartofMolecular and Cellular Endocrinology 339 (2011) 45–53en
dc.rights© 2011 Elsevier Ireland Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0.en
dc.subjectGene regulationen
dc.subjectTranscription factorsen
dc.titleTranscriptional regulation of type 11 17β-hydroxysteroid dehydrogenase expression in prostate cancer cellsen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Ciencias de la Saludes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Osasun Zientziak Sailaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes

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© 2011 Elsevier Ireland Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0.
Except where otherwise noted, this item's license is described as © 2011 Elsevier Ireland Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0.