Resumen
The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources. The study revealed that this white-rot basidiomycete attacks all the components of the plant cell wall. P. ostreatus secretes a variety of glycoside ...
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The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources. The study revealed that this white-rot basidiomycete attacks all the components of the plant cell wall. P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source. The presence of wood increased the secretome complexity, whereas glucose diminished the secretion of enzymes involved in cellulose, hemicellulose and pectin degradation. In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes. The comparison of the secretomes of monokaryons and dikaryons reveals that secretome complexity is unrelated to the nuclear composition of the strain. [--]
Materias
Fungal biology,
Proteomics
Publicado en
Scientific Reports, 2020, 10:12421
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB
Entidades Financiadoras
Grants numbers AGL2014-55971-R (AEI, FEDER, UE) and RTI2018-099371-B-I00 (MCIU/AEI/FEDER, UE) of the Spanish National Research Plan, and PRO-UPNA18 from the Public University of Navarre have funded this research. Proteomics work in Goettingen was supported within the framework of a Common Lower Saxony-Israel-Project (ZN2043) by the Ministry of Science and Culture of Lower Saxony granted to UK and AM. MA appreciates a grant for research exchanges in Goettingen and a postdoctoral grant by Universidad Pública de Navarra. /
Grants numbers AGL2014-55971-R (AEI, FEDER, UE) and RTI2018-099371-B-I00 (MCIU/AEI/FEDER, UE) of the Spanish National Research Plan, and PRO-UPNA18 from the Public University of Navarre have funded this research. Proteomics work in Goettingen was supported within the framework of a Common Lower Saxony-Israel-Project (ZN2043) by the Ministry of Science and Culture of Lower Saxony granted to UK and AM. MA appreciates a grant for research exchanges in Goettingen and a postdoctoral grant by Universidad Pública de Navarra. Some of these experiments also featured in the thesis of Manuel Alfaro.