Noncontiguous operon atlas in Staphylococcus aureus
Consultable a partir de
2025-12-01
Fecha
2023Autor
Director
Versión
Acceso embargado 2 años / 2 urteko bahitura
Tipo
Tesis doctoral / Doktoretza tesia
Impacto
|
10.48035/Tesis/2454/46910
Resumen
In previous work from the group, we described the existence of a new transcriptional
architecture that we call a noncontiguous operon (NcO). NcOs consist of groups of
genes that are transcribed on the same RNA molecule, although genes that are
transcribed in the opposite direction separate them. The mRNA of the gene
transcribed in the opposite direction is full-length complementary and therefore
...
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In previous work from the group, we described the existence of a new transcriptional
architecture that we call a noncontiguous operon (NcO). NcOs consist of groups of
genes that are transcribed on the same RNA molecule, although genes that are
transcribed in the opposite direction separate them. The mRNA of the gene
transcribed in the opposite direction is full-length complementary and therefore
antisense to the mRNA of the operon. Unlike classical operons, whose presence in
the genome is predicted based on theoretical criteria of proximity between genes and
biological function of the encoded proteins, the presence of NcOs cannot be
determined theoretically and it is necessary to resort to transcriptomic analyses to
demonstrate the co-transcription of non-contiguous groups of genes. As a
continuation of this line of research, in this thesis we decided to determine the map
of NcOs present in the genome of a bacterium. This information would allow us to
know the abundance of this transcriptional architecture and thus analyze whether
they have common elements or related functions. We decided to use Staphylococcus
aureus as a model for four reasons: (I) it is the bacterium in which we first described
this transcriptional architecture, (II) we had transcriptomic data obtained from the
Illumina platform that could be useful in our study, (III) we have experience in its
genetic manipulation and (IV) it has enormous clinical relevance due to its versatility
as a pathogen and the existence of multidrug-resistant bacteria. [--]
Materias
Staphylococcus aureus,
Noncontiguous operons,
Gene expression
Departamento
Universidad Pública de Navarra. Departamento de Ciencias de la Salud /
Nafarroako Unibertsitate Publikoa. Osasun Zientziak Saila