Artículos de revista DABA - ABES Aldizkari artikuluak

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Browsing Artículos de revista DABA - ABES Aldizkari artikuluak by Subject "AcMNPV"

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    PublicationOpen Access
    The parasitoid hyposoter didymator can transmit a broad host range baculovirus in a two host system
    (MDPI, 2023) Morel, Ariel; Leigh, Brendan; Muñoz Labiano, Delia; Caballero Murillo, Primitivo; Medina, Pilar; Dáder, Beatriz; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura
    Hyposoter didymator (Thunberg) (Hymenoptera: Ichneumonidae) and baculovirus (BV) might be used jointly to provide effective control of the Spodoptera genus. The literature has mostly covered the safe compatibility between natural enemies and BV-based insecticides, but research on the potential dispersal of BV by natural enemies is lacking. Thus, the goal of this manuscript was to ascertain if H. didymator was able to disperse the broad host range of Autographa californica nucleopolyhedrovirus (AcMNPV) to Spodoptera littoralis and Spodoptera exigua in choice and non-choice conditions and whether the preference of the parasitoid by one of these noctuids could mediate this dispersion. It was previously needed to improve the rearing of the parasitoid in the laboratory, concerning the optimal host age and length of parasitization, parasitoid competition, and influence of parasitization on the longevity of females. The best rearing conditions for S. littoralis are collective parasitization of mature L3 larvae for 24 h, after at least one day of copulation. Hyposoter didymator transmits AcMNPV to both lepidopterans, but its efficiency is mediated by host preference and the pathogenicity of the BV in each host. In this particular case, H. didymator as well as AcMNPV showed a clear preference towards S. exigua.
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    PublicationOpen Access
    The role of Chrysoperla carnea (Steph.) (neuroptera: Chrysopidae) as a potential dispersive agent of noctuid baculoviruses
    (MDPI, 2020) Gutiérrez Cárdenas, Oscar Giovanni; Adán, Ángeles; Beperet Arive, Inés; Medina, Pilar; Caballero Murillo, Primitivo; Garzón, Agustín; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura
    Baculoviruses (BV) are highly effective against lepidopteran pests of economic importance such as Spodoptera exigua. The combined use of entomopathogens and macrobiological control agents requires the study of their relationships. Laboratory bioassays were developed to evaluate the interactions between the multiple nucleopolyhedroviruses of S. exigua (SeMNPV) and Autographa californica (AcMNPV), and the predator Chrysoperla carnea. The microscopic examination of predator’s excreta (larval drops and meconia) after the ingestion of BV-infected S. exigua revealed the presence of viral occlusion bodies (OBs). The reinfection of S. exigua larvae with BVs-contaminated excreta by using OBs water suspensions or by direct application both yielded high mortality values but different speed-of-kill results. Meconia killed before in suspensions due to their higher viral load and larval excretion drops did so in direct application due to their liquid nature and their easiness of consumption. The prey-mediated ingestion of SeMNPV and AcMNPV triggered slight effects in C. carnea, which were probably derived from the food nutritional quality. Chrysoperla carnea larvae did not discriminate between healthy and BV-infected S. exigua, while a preference was shown for S. exigua (healthy or infected) vs. Macrosiphum euphorbiae. Our findings present C. carnea, and particularly its larvae, as a promissory candidate for BV dispersion in the field.