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Publication Open Access El fruto de la acerola: composición y posibles usos alimenticios(Sociedad Latinoamericana de Nutrición, 2006) Mezadri, Tatiana; Fernández-Pachón, María Soledad; Villaño Valencia, Débora; García-Parrilla, María Carmen; Troncoso, Ana M.; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODEl objetivo de esta revisión es caracterizar el fruto de la acerola, detallando así sus aspectos taxonómicos, vegetativos, de composición y de mercado. También se evalúa su posible utilización como ingrediente alimentario en vistas a la producción de alimentos funcionales. Para la realización de esta revisión bibliográfica se han consultado bases de datos internacionales (Scifinder Scholar y Medline) y artículos originales que han sido localizados y proveídos principalmente por los Recursos Electrónicos de la Biblioteca de la Universidad de Sevilla y la Universidad do Vale do Itajaí (Santa Catarina, Brazil). La acerola (Malpighia emarginata Sessé y Mociño ex DC) es una especie arbustiva que se desarrolla en zonas de climas tropical y subtropical. Su origen se centra en el sur de México, América Central y zona septentrional de Sudamérica. Su denominación se adoptó en 1986 por el Consejo Internacional de Recursos Genéticos Vegetales. Malpighia emarginata presenta un fruto subglobuloso en forma de drupa, el cual posee tres semillas que representan entre el 19 y el 25% del peso total. El diámetro del fruto varía de 1 a 4 cm y el peso de 2 a 15 g. Presenta una coloración verde cuando está en desarrollo, cambiando a tonos amarillos y rojos cuando está maduro. La maduración ocurre en corto espacio de tiempo. El período de fructificación es de 3 a 4 veces al año. Cada planta produce cerca de 20 a 30 kg de frutos anualmente. La fruta de acerola proporciona macro y micronutrientes: proteínas (0,21 g/100 g), grasas (0,23 g/100 g), carbohidratos (3,57 g/100 g), sales minerales (hierro (0,24 mg/100 g, calcio 11,7 mg/100 g, fósforo 17,1 mg/100g) y vitaminas (tiamina (0,02 mg/100 g, riboflavina 0,07 mg/100 g, piridoxina 8,7 mg/100 g). Hay que destacar su elevado contenido en vitamina C (695 a 4827 mg/100 g), el cual ha inducido un gran consumo de esta fruta en los últimos años, de ahí su importante valor económico. La acerola también presenta carotenoides y bioflavonoides, que le otorgan gran valor nutritivo y su uso potencial como antioxidante. La composición de la fruta depende de factores geográficos, agrícolas y de procesado. Brasil, por su clima y suelo favorables, es el principal productor mundial de acerola, que comercializa en forma de zumos, mermeladas, helados, compotas, gelatinas, confituras, dulces y licores. Debido a la heterogeneidad que presenta la acerola, resulta útil a nivel comercial seleccionar y clonar las variedades más óptimas por su valor nutritivo y su palatabilidad.Publication Open Access Revisión de los métodos de evaluación de la actividad antioxidante in vitro del vino y valoración de sus efectos in vivo(Sociedad Latinoamericana de Nutrición, 2006) Fernández-Pachón, María Soledad; Villaño Valencia, Débora; Troncoso, Ana M.; García-Parrilla, María Carmen; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODEl objetivo de esta revisión es el estudio de la actividad antioxidante del vino, analizando los distintos métodos que se han empleado en su evaluación. Esta actividad se relacionará, tanto desde el punto de vista cualitativo como cuantitativo, con el perfil de compuestos polifenólicos presentes en el vino. Los vinos tintos presentan actividades superiores a los blancos y la magnitud de dichos valores varía en función del método de análisis empleado. No existe un único compuesto polifenólico responsable mayoritariamente de la actividad antioxidante del vino, sino que ésta se explica por el conjunto de todos ellos. Para evaluar la influencia del consumo de vino tinto en el organismo humano se ha utilizado como biomarcador más frecuente la capacidad antioxidante del plasma, que incrementa su valor tras la ingesta del vino. Se expone que la evaluación de la actividad antioxidante in vitro del vino requiere el uso de diversos métodos que proporcionen una información diferente y complementaria. Los métodos in vivo tienen la ventaja de valorar las transformaciones metabólicas que sufren los fenoles en el organismo y que modifican su actividad. De los estudios realizados hasta la fecha se concluye que el vino interviene en la capacidad antioxidante del plasma de forma directa a través de sus compuestos polifenólicos e indirecta al aumentar la concentración plasmática de ácido úrico.Publication Open Access Effect of preservative agents on the respiration rate of minimally processed potato (Solanum tuberosum cv. Monalisa)(Institute of Food Technologists, 2008) Petri, E.; Arroqui Vidaurreta, Cristina; Angós Iturgaiz, Ignacio; Virseda Chamorro, Paloma; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODPublication Open Access Occurrence of patulin and its dietary intake through apple juice consumption by the Spanish population(Elsevier, 2009) Amézqueta, Susana; González-Peñas, Elena; López de Cerain, Adela; Murillo Arbizu, María Teresa; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODPublication Open Access Ochratoxin A decontamination: a review(Elsevier, 2009) Amézqueta, Susana; González-Peñas, Elena; Murillo Arbizu, María Teresa; López de Cerain, Adela; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD; Agronomía, Biotecnología y AlimentaciónPublication Open Access Comparison between capillary electrophoresis and high performance liquid chromatography for the study of the occurrence of patulin in apple juice intended for infants(Elsevier, 2010) Murillo Arbizu, María Teresa; González-Peñas, Elena; Amézqueta, Susana; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD; Agronomía, Biotecnología y AlimentaciónPublication Open Access Occurrence of ochratoxin A in southern Spanish generous wines under the denomination of origin "Jerez-Xérès-Sherry and 'Manzanilla' Sanlúcar de Barrameda"(MDPI, 2010) Murillo Arbizu, María Teresa; Amézqueta, Susana; González-Peñas, Elena; López de Cerain, Adela; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Gobierno de Navarra / Nafarroako GobernuaThe mycotoxin ochratoxin A (OTA) has toxic effects in animals; the most relevant of them is nephrotoxicity. OTA has also been classified as a possible carcinogen for humans (group 2B) by the International Agency for Research on Cancer (IARC). Therefore, exposure to OTA through contaminated food can represent health impairment to humans. The maximum permitted level for this mycotoxin in wine is 2.0 g/L. The presence of OTA in Spanish wines produced using the traditional methods under the Denomination of Origin “Jerez-Xérès-Sherry and manzanilla Sanlúcar de Barrameda” was evaluated by a High performance Liquid Chromatography method with fluorescence detection and immunoaffinity column purification. A recovery of 95.4% and a limit of detection and quantification of 0.009 g/L and 0.02 g/L respectively, were achieved. In manzanilla, fino, amontillado and oloroso wine, the mean OTA values were 0.042, 0.044, 0.144, and 0.319 g/L, respectively. These levels are not different from other data given in the reference literature on white wines, although fino and manzanilla wines have very low OTA levels.Publication Open Access Alginate edible coating and cold storage for improving the physicochemical quality of cape gooseberry (Physalis peruviana l.)(Herald Scholarsly Open Access, 2015) Pedro Carvalho, Catarina; Villaño Valencia, Débora; Moreno, Diego A.; Serrano, María; Valero, Daniel; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODThe Cape gooseberry is an exotic tropical fruit and, nowadays, is the second most exported fruit from Colombia. Therefore, the high demand for quality required research for a better understanding of fruit behavior. Furthermore, postharvest quality properties play an important role in meeting consumer demands. Cold storage and edible coatings are reported as efficient technologies for extending shelf life and preserve the quality of fruits in postharvest. As there are no reports of studies about the effect of these technologies on shelf life, quality and antioxidant activity in Cape gooseberry, this work aim to evaluate the use of alginate 1% during 21 days of storage at 2ºC as an alternative for postharvest handling of this fruit. Cape gooseberry exhibits a high respiration rate and ethylene production at 20ºC. Alginate coat decreased significantly the metabolism activity of fruit during the cold storage without change significantly the fruit organoleptic quality and showing total phenolic, carotenoid contents and antioxidant activity. Alginate is an efficient edible coat for preserve the quality and bioactivity of Cape gooseberry during 21 days of storage at 2ºC.Publication Open Access Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods(Society of Chemical Industry Elsevier Applied Science Publishers, 2015) Bobo García, Gloria; Davidov Pardo, Gabriel; Arroqui Vidaurreta, Cristina; Virseda Chamorro, Paloma; Marín Arroyo, Remedios; Navarro Huidobro, Montserrat; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODPublication Open Access Antioxidant properties of leaves from different accessions of grapevine (vitis vinifera L.) cv. tempranillo after applying biotic and/or environmental modulator factors(Elsevier, 2015-01-17) Torres Molina, Nazareth; Antolín, M. Carmen; Goicoechea, Nieves; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta ElikaduraWithin climate change scenario, the maintenance of grape quality and wine characteristics will be the main concern for viticulture in the future years. However, changes in the composition of grapevine pruning wastes (i.e., leaves and stems) could be another interesting aspect as important antioxidant source for pharmaceutical industry due its richness in phenolic compounds beneficial for human health. To date, the effect of biotic and environmental factors in the accumulation of these compounds in leaves had received little attention. Therefore, the aims of study were (1) to evaluate the effect of biotic (mycorrhizal inoculation) and environmental (temperature) factors, alone or combined, on phenolic composition and antioxidant activity of leaf extracts of grapevine and (2) to determine whether such effects differed among accessions of the same cultivar of grapevine. The study was carried out using container-grown grapevines grown in greenhouses. Dormant Vitis vinifera (L.) cuttings of different accessions of Tempranillo were selected to get fruit-bearing cuttings. At transplanting, half of the plants of each accession were inoculated with the mycorrhizal inoculum and after fruit set, plants were exposed to two temperature regimes (24 °C/14 °C and 28 °C/18 °C (day/night)) to commercially berry ripe. Results showed that total phenolic content, antioxidant compounds like flavonols and anthocyanins, and antioxidant activity of leaves were improved with mycorrhizal inoculation under high temperature conditions. It was concluded that mycorrhizal inoculation of grapevines could contribute to preserve high level of antioxidant compounds of leaves in a future climate change scenario. However, the effects were strongly dependent of accession assayed, which indicated a significant intra-varietal diversity in the response of Tempranillo to biotic and environmental factors.Publication Open Access Phenolic profile and biological activities of the pepino (solanum muricatum) fruit and itswild relative S. caripense(MDPI, 2016) Herraiz, Francisco J.; Villaño Valencia, Débora; Plazas, Mariola; Vilanova, Santiago; Ferreres, Federico; Prohens, Jaime; Moreno Fernández, Diego Ángel; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODThe pepino (Solanum muricatum) is an edible and juicy fruit native to the Andean region which is becoming increasingly important. However, little information is available on its phenolic composition and bioactive properties. Four pepino varieties (37-A, El Camino, Puzol, and Valencia) and one accession (E-7) of its close wild relative S. caripense were characterized by HPLC-DAD-MSn/ESI. Twenty-four hydroxycinnamic acid derivatives were detected (5 to 16 compounds per variety or accession), with differences of more than two-fold for their total content among the materials studied. The major phenolics in the pepino varieties were chlorogenic acids and derivatives, while in S. caripense a caffeoyl-synapoyl-quinic acid was the major compound. The in vitro antioxidant capacity (DPPH (2,2-diphenyl-1-picrylhydrazyl hydrate), ORAC (oxygen radical absorbance capacity), and TRC (total reducing capacity) tests) was higher in S. caripense. Pepino and S. caripense extracts were not toxic for RAW 264.7 macrophage cells, and the raw extracts inhibitedNOproduction of the lipopolysaccharide (LPS)-stimulated macrophages by 36% (El Camino) to 67% (37-A). No single variety ranked high simultaneously for hydroxycinnamic acids content, antioxidant activity and biological activity. We suggest the screening of large collections of germplasm or the use of complementary crosses between Puzol (high for hydroxycinnamic acids and biological activity) and S. caripense E-7 (high for antioxidant activity) to select and breed pepino varieties with enhanced properties.Publication Open Access Relationship between the ingestion of a polyphenol-rich drink, hepcidin hormone, and long-term training(MDPI, 2016) Villaño Valencia, Débora; Vilaplana, Cristina; Medina, Sonia; Algaba-Chueca, Francisco; Cejuela-Anta, Roberto; Martínez-Sanz, José Miguel; Ferreres, Federico; Gil-Izquierdo, Ángel; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODThe effects of polyphenol-rich foods on the iron status of athletes, as well as the effect of physical training on the hormone hepcidin, implicated in iron metabolism, are not clear. We investigated the influence on iron metabolism of a long-term training intervention of 120 days, measuring the hepcidin concentration in the plasma of 16 elite triathletes, and the effect of the ingestion of 200 mL of either aronia-citrus juice or a placebo drink for 45 days, in a crossover design. The highest plasma hepcidin concentrations were observed at the beginning of the study (116 ± 63 nM) and levels steadily decreased until the end of the intervention (final value 10 ± 7.5 nM). Long-term training might reduce inflammation and, hence, could be responsible for the decrease in hepcidin in triathletes. Polyphenols from aronia-citrus juice did not interfere in iron absorption, as we did not observe significant differences between the intake of the placebo drink or juice with regard to hepcidin levels. Further studies are required to ascertain the time and conditions necessary to restore hepcidin levels, which reflect the iron status of triathletes.Publication Open Access Mathematical modeling of thin layer drying of green plantain (Musa paradisiaca L.) peel(Universiti Putra Malaysia, 2016) Faneite, A. M.; Rincón, A.; Ferrer, A.; Angós Iturgaiz, Ignacio; Argüello, G.; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODPublication Open Access Berry quality and antioxidant properties in vitis vinifera cv. tempranillo as affected by clonal variability, mycorrhizal inoculation and temperatura(CSIRO Publishing, 2016-08-24) Torres Molina, Nazareth; Goicoechea, Nieves; Morales Iribas, Fermín; Antolín, M. Carmen; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta ElikaduraThe projected increase in mean temperatures caused by climate change is expected to have detrimental impacts on berry quality. Microorganisms as arbuscular mycorrhizal fungi (AMF) produce numerous benefits to host plants and can help plants to cope with abiotic stresses such as high temperature. The aims of this research were to characterise the response of three clones of Vitis vinifera L. cv. Tempranillo to elevated temperatures and to determine whether AMF inoculation can improve berry antioxidant properties under these conditions. The study was carried out on three fruit-bearing cuttings clones of cv. Tempranillo (CL-260, CL-1048 and CL-1089) inoculated with AMF or uninoculated and subjected to two temperature regimes (day¿night: 24°C¿14°C and 28°C¿18°C) during berry ripening. Results showed that clonal diversity of Tempranillo resulted in different abilities to respond to elevated temperature and AMF inoculation. In CL-1048, AMF inoculation improved parameters related to phenolic maturity such as anthocyanin content and increased antioxidant activity under elevated temperature, demonstrating a protective role of AMF inoculation against warming effects on berry quality. The results therefore suggest that selection of new clones and/or the implementation of measures to promote the association of grapevines with AMF could be strategies to improve berry antioxidant properties under future warming conditions.Publication Open Access Antioxidant, anti-inflammatory, and microbial-modulating activities of nutraceuticals and functional foods(Hindawi, 2017) Peluso, Ilaria; Magrone, Thea; Villaño Valencia, Débora; Chen, Oliver; Palmery, Maura; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODThis special issue highlights the importance of using different methodological approaches in order to clarify the mechanisms of the potential health effects of nutraceuticals.Publication Open Access Soil and climate determine antioxidant capacity of walnuts(United Arab Emirates University, 2017) Figueroa, Fernando; Marhuenda, Javier; Gironés-Vilaplana, Amadeo; Villaño Valencia, Débora; Martínez-Cachá, Adela; Mulero, Juana; Cerdá, Begoña; Zafrilla, Pilar; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODThe aim of this work was to study the influence of soil and climate factors in the antioxidant capacity of 10 different varieties of nuts, obtained from two experimental cultivars with different weather and soil conditions. Ten different varieties of walnut samples were obtained from two experimental cultivars, analyzing the antioxidant capacity by ORAC, DPPH• and ABTS+ methods. Eureka and Tehama walnuts showed the highest antioxidant capacity. Only Chico and Serr varieties (measured by ABTS+ method) and Franquette variety (measured by ORAC assay) increased their antioxidant capacity when cultured in Torre Blanca plots. The growing in El Chaparral plots increased the antioxidant capacity of every different variety. Minor temperature (especially when it reaches over -0 ºC) and humidity, as well as an increase on the annual rainfall are noteworthy factors that influence on the increase of antioxidant capacity of the wide majority of the analyzed walnuts.Publication Open Access Rational application of treated sewage sludge with urea increases GHG mitigation opportunities in Mediterranean soils(Elsevier, 2017-02-01) Calleja Cervantes, María Eréndira; Aparicio Tejo, Pedro María; Villadas Latorre, Pablo José; Irigoyen Iriarte, Ignacio; Irañeta, Iosu; Fernández-González, A.J.; Fernández-López, M.; Menéndez, S.; Producción Agraria; Nekazaritza Ekoizpena; Ciencias del Medio Natural; Natura Ingurunearen Zientziak; Institute for Multidisciplinary Research in Applied Biology - IMABPublication Open Access Flavonoid and amino acid profiling on vitis vinifera L. cv tempranillo subjected to deficit irrigation under elevated temperaturas(Elsevier, 2017-05-03) Torres Molina, Nazareth; Hilbert, Ghislaine; Luquin, Josu; Goicoechea, Nieves; Antolín, M. Carmen; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta ElikaduraThroughout the southern Mediterranean regions of Europe, projected climate warming combined with severe droughts during the growing season may alter grape metabolism, thus modifying the nutritional value of berries and the quality of wines. This study investigated the effects of pre- and post-veraison drought under elevated temperatures on berry skin metabolism of two Tempranillo clones (CL). Experimental assays were performed on fruit-bearing cuttings from CL-1089 and CL-843 of Vitis vinifera (L.) cv. Tempranillo subjected to two temperature regimes (24/14 °C or 28/18 °C (day/night)) combined with three irrigation regimes during berry ripening: (i) water deficit from fruit set to veraison (early deficit, ED); (ii) water deficit from veraison to maturity (late deficit, LD); and (iii) full irrigation (FI). At 24/14 °C, the LD treatment performed better than the ED treatment. Differences were attenuated at 28/18 °C and responses were modulated by type of clone. Elevated temperatures induced the accumulation of hexoses and amino acids in berries. ED at 24/14 °C reduced anthocyanins and flavonols, which may decrease the antioxidant properties of fruits. In contrast, the levels of these secondary metabolites did not decrease when LD was applied. Our results suggest that the adaptation of grapevines for climate change might be plausible with the optimization of timing of water deficit and the appropriate selection of clones.Publication Open Access Sampling stratification using aerial imagery to estimate fruit load in peach tree orchards(MDPI, 2018) Miranda Jiménez, Carlos; Santesteban García, Gonzaga; Urrestarazu Vidart, Jorge; Loidi Erviti, Maite; Royo Díaz, José Bernardo; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta ElikaduraA quick and accurate sampling method for determining yield in peach orchards could lead to better crop management decisions, more accurate insurance claim adjustment, and reduced expenses for the insurance industry. Given that sample size depends exclusively on the variability of the trees on the orchard, it is necessary to have a quick and objective way of assessing this variability. The aim of this study was to use remote sensing to detect the spatial variability within peach orchards and classify trees into homogeneous zones that constitute sampling strata to decrease sample size. Five mature peach orchards with different degrees of spatial variability were used. A regular grid of trees was established on each orchard, their trunk cross-sectional area (TCSA) was measured, and yield was measured as number of fruits/tree on the central tree of each one of them. Red Vegetation Index (RVI) was calculated from aerial images with 0.25 m pixel -1 resolution, and used, either alone or in combination with TCSA, to delineate sampling strata using cluster fuzzy k-means. Completely randomized (CRS) and stratified samplings were compared through 10,000 iterations, and the Minimum Sample Size required to obtain estimates of actual production for three quality levels of sampling was calculated in each case. The images allowed accurate determination of the number of trees, allowing a proper application of completely randomized sampling designs. Tree size and the canopy density estimated by means of multispectral indices are complementary parameters suitable for orchard stratification, decreasing the sample size required to determine fruit count up to 20–35% compared to completely randomized samples.Publication Open Access Effect of linseed supplementation and slaughter age on meat quality of grazing cross-bred Galician x Burguete foals(Wiley, 2018) Domínguez, Rubén; Pateiro, Mirian; Crecente, Santiago; Ruiz Darbonnens, Marta; Sarriés Martínez, María Victoria; Lorenzo, José M.; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOODBACKGROUND: The aim of this study was to assess the effect of finishing diet (control concentrate vs. linseed concentrate) andslaughter age (13 vs. 26 months) on meat and nutritional quality of foal meat. For this study, 46 foals from crossing GaliciaMountain x Burguete breeds were used.RESULTS: The obtained results showed that slaughter age had an influence on chemical composition and colour parameters.Foals slaughtered at the age of 13 months had lower content of intramuscular fat and higher cholesterol contents than thoseslaughtered at 26 months of age. Regarding colour parameters, older foals showed the highest values of redness and lowestmyoglobin contents. Finishing diet had a low effect on the majority of parameters evaluated. Foals fed with linseed presentedlower shear force values than those fed with control concentrate. Fatty acid and amino acid contents were hardly influenced byfinishing diet, whereas slaughter age effect had a high impact on fatty acid profile. Older animals showed lowest SFA values andn-6/n-3 ratio.CONCLUSION: Older animals presented the best meat quality. Diet had low effect in meat quality and could be related to theshort time during finishing diet was administered and the low amount of linseed in the experimental diet.