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Marzo Pérez, Florencio

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Marzo Pérez

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Florencio

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Ciencias del Medio Natural

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0000-0001-6428-9382

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18

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2003 - 20085
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    PublicationOpen Access
    Local (gut) and systemic metabolism of rats is altered by consumption of raw bean (Phaseolus vulgaris L. var. athropurpurea)
    (Cambridge University Press, 2003) Cavallé de Moya, Carmen; Grant, George; Frühbeck, Gema; Urdaneta, Elena; García, María; Marzo Pérez, Florencio; Santidrián, Santiago; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
    The composition of the raw legume Phaseolus vulgaris L. var. athropurpurea (PhVa) and its effects on the metabolism of young growing rats have been evaluated. The levels of protein, unsaturated fatty acids, carbohydrate, fibre and bioactive factors present in PhVa were comparable with those in other Phaseolus vulgaris varieties. However, the lectins of PhVa were predominantly of the leucoagglutinating type, and concentrated in the albumin protein fraction. Rats fed a diet (110 g total protein, 16·0 M/g) in which PhVa meal provided about half of the protein excreted high levels of N in faeces and urine, and grew more slowly, than rats fed a high-quality control diet (ad libitum or pair-fed). Small intestine, large intestine and pancreas weights were increased (by almost 100 %, P<0·05), whilst skeletal muscle, thymus and spleen weights were reduced. Blood insulin 16·20 v. 0·50 m/, P<0·05, thyroxine, glucose, protein (60·5 v. 48·3 /, P<0·05) and LDL-cholesterol were lowered, whilst glucagon (155·3 v. 185·4 n/, P<0·05), triiodothyronine and urea were elevated, as were urinary urea, creatinine and glucose. These changes in the local (gut) and systemic metabolism of rats were probably mediated primarily by lectins in PhVa, which were concentrated in the albumin protein fraction, whereas in many other Phaseolus vulgaris lines they are distributed across the globulin and albumin fractions.
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    PublicationOpen Access
    Nutritional value of raw and extruded chickpeas (Cicer arietinum L.) for growing chickens
    (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2008) Brenes, A.; Viveros, A.; Centeno, C.; Arija, I.; Marzo Pérez, Florencio; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
    Se realizó un experimento con el objeto de estudiar el efecto de la inclusión de distintas concentraciones (0, 100, 200 y 300 g kg-1) de garbanzo crudo y extrusionado sobre los parámetros productivos, el peso y la longitud de los órganos digestivos y la digestibilidad de la proteína y la grasa de pollos broiler (0-21 días de edad). Los datos fueron analizados siguiendo un diseño factorial (3 x 2) con tres concentraciones de garbanzo con o sin extrusión. Se utilizó una dieta basada en maízsoja como control positivo sin garbanzo. La inclusión de cantidades crecientes de garbanzo en la dieta no modificó la ganancia de peso, el consumo de alimento ni el índice de transformación de las aves, pero sí los pesos relativos del páncreas e hígado y las longitudes relativas del duodeno, yeyuno, íleon y ciego que se incrementaron significativamente (P<0,05). La digestibilidad aparente ileal (AID) de la proteína bruta (CP) y la digestibilidad aparente fecal (AED) de la grasa bruta (CF) se incrementaron (P<0,05) sólo en el caso de la incorporación de 200 g kg-1 de garbanzo. La extrusión mejoró la ganancia de peso de las aves, la AID (P<0,001) de la CP y la AED de la CF y disminuyó (P<0,05) el peso relativo del páncreas. En conclusión, la inclusión de hasta 300 g kg-1 de garbanzo no produjo modificaciones en los parámetros productivos de las aves y causó efectos negativos en algunos órganos digestivos.
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    PublicationOpen Access
    Nutritional value of protein from vegetative mycelia of edible mushroom Pleurotus ostreatus
    (Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006) Parada Albarracín, Julián Andrés; Urdaneta, Elena; Marzo Pérez, Florencio; Ramírez Nasto, Lucía; Pisabarro de Lucas, Gerardo; Producción Agraria; Nekazaritza Ekoizpena; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
    The present work was designed to study the effects of supplementation a control diet with P. ostreatus mycelium for evaluation a nutritional value of mycoprotein and possible cholesterol lowering.
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    PublicationOpen Access
    Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
    (BioMed Central, 2008) Cordero, P.; Campión, J.; Milagro, F. I.; Marzo Pérez, Florencio; Martínez, J. A.; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
    The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis of glucose. Therefore, 20 mice weighing 23.37 +/- 0.96 g were hydrodinamically gene transferred by administering into the tail vein a bolus with ICL and MS. After 36 hours, body weight, plasma glucose, respiratory quotient and energy expenditure were measured. The respiratory quotient was increased by gene transfer, which suggests that a higher carbohydrate/lipid ratio is oxidized in such animals. This application could help, if adequate protocols are designed, to induce fat utilization of obesity and diabetes.
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    PublicationOpen Access
    Effect of dietary quercetin and sphingomyelin on intestinal nutrient absorption and animal growth
    (Cambridge University Press, 2006) Barrenetxe, Jaione; Aranguren Garacochea, Patricia; Grijalba, A.; Martínez-Peñuela, J. M.; Marzo Pérez, Florencio; Urdaneta, Elena; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
    Research on cancer and other conditions has shown flavonoids and sphingolipids to be food components capable of exerting chemoprotective action. Nevertheless, little is known about their effects on healthy individuals and their potential usefulness as therapeutic agents. The present study examined the possible action of a dietary flavonoid, quercetin, and a sphingolipid, sphingomyelin, as functional foods in healthy animals. In particular, the effect on animal growth of supplementing a conventional diet with one or other of these substances (0·5% quercetin and 0·05% sphingomyelin) was considered. Possible action affecting intestinal physiology was also analysed by measuring the uptake of sugar and dipeptide, mediated by the Na+-dependent sugar transporter SGLT1 and the dipeptide Na+/H+ exchanger PEPT1 respectively, and the activity of related intestinal enzymes such as sucrase, maltase and aminopeptidase N. Both substances seemed to modify small intestinal activity in healthy mice, altering intestinal enzymatic activity and nutrient uptake. These effects observed in the small intestine did not impair normal development of the animals, as no differences in serum biochemical parameters or in organ and body weights were found. The findings should help in elucidating the mechanisms of action of these food components with a view to their possible use in the prevention of certain pathological conditions.