Open Access
Optimización de dispositivos en fibra óptica basados en resonancias de modos con pérdidas para aplicaciones en biosensores
(2018) Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Arregui San Martín, Francisco Javier; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa; Gobierno de Navarra / Nafarroako Gobernua PC023-024 BIOPTSENS
El desarrollo de los sensores en fibra óptica combinado con el avance de la nanotecnología ha proporcionado una excelente oportunidad para el desarrollo de nuevas plataformas sensoras ópticas portátiles, ligeras y de alto rendimiento capaces de realizar detección in situ y en tiempo real. Esta tesis quiere contribuir al avance de la tecnología de los sensores ópticos basados en resonancias de modos con pérdidas (LMRs, por sus siglas en inglés), mejorando el rendimiento de dichos dispositivos, para luego detectar biomarcadores específicos que permitan un diagnóstico selectivo y precoz de diferentes enfermedades. En este trabajo, los LMRs generados en una fibra de tipo D permiten medir los cambios en el índice de refracción externo con una sensibilidad muy alta. En consecuencia, es posible detectar concentraciones muy bajas de analito en muestras de suero. Los resultados mostrados en esta tesis revelan el potencial de los sensores basados en LMRs para desempeñar un papel fundamental en el ámbito biomédico.
Open Access
Optical sensors based on lossy-mode resonances
(Elsevier Science, 2017) Matías Maestro, Ignacio; Ascorbe Muruzabal, Joaquín; Acha Morrás, Nerea de; López Torres, Diego; Zubiate Orzanco, Pablo; Sánchez Zábal, Pedro; Urrutia Azcona, Aitor; Socorro Leránoz, Abián Bentor; Rivero Fuente, Pedro J.; Hernáez Sáenz de Zaitigui, Miguel; Elosúa Aguado, César; Goicoechea Fernández, Javier; Bariáin Aisa, Cándido; Corres Sanz, Jesús María; Ruiz Zamarreño, Carlos; Arregui San Martín, Francisco Javier; Del Villar, Ignacio; Ingeniería Eléctrica y Electrónica; Ingeniaritza Elektrikoa eta Elektronikoa; Institute of Smart Cities - ISC
Open Access
Thin film coated D-shaped fiber regenerable biosensor
(Optica, 2020) Santano Rivero, Desiree; Ciáurriz Gortari, Paula; Tellechea Malda, Edurne; Zubiate Orzanco, Pablo; Socorro Leránoz, Abián Bentor; Del Villar, Ignacio; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza; Institute of Smart Cities - ISC
We present a novel covalent functionalization of a D-shape fiber biosensor based on Lossy Mode Resonances. IgG/anti-IgG model is applied to prove the regeneration of the union and thus the re-usability of the sensor.
Embargo
Regenerable LMR-based fiber optic immunosensor with a SnO2 metallic oxide thin film for label-free detection
(Pergamon Press, 2025-05-15) Santano Rivero, Desiree; Zubiate Orzanco, Pablo; Socorro Leránoz, Abián Bentor; Del Villar, Ignacio; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza; Institute of Smart Cities - ISC; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
This paper introduces the fabrication and characterization of a regenerable LMR-based, label-free optical fiber immunosensor. This innovative biosensor proposal was developed by functionalizing a SnO2 metallic oxide thin film deposited on a D-shaped optical fiber using a silanization protocol. The system successfully detected IgG - anti-IgG complexes in real-time in a range of concentrations from 0.5 to 10 ¿g/ml and achieved a limit of detection (LoD) of 0.12 μg/ml of anti-IgG. The biosensor was extensively tested to assess its capacity for regeneration, confirming that it can be reused repeatedly, reducing the overall cost and waste typically associated with disposable sensors. This regenerability has significant implications for a range of applications, providing a more sustainable and flexible approach to biosensing technology.
Open Access
Ultra-low detection limit lossy mode resonance-based fibre-optic biosensor
(Optica Publishing Group, 2018) Chiavaioli, Francesco; Zubiate Orzanco, Pablo; Del Villar, Ignacio; Ruiz Zamarreño, Carlos; Giannetti, Ambra; Tombelli, Sara; Trono, Cosimo; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Baldini, Francesco; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza; Institute of Smart Cities - ISC
We report on ultra-low detection limit lossy mode resonance-based fibre-optic biosensor coated with nm-thick tin dioxide film, which allows measuring fibre-surrounding medium changes with very high sensitivity for label-free and real-time biomolecular interaction analysis
Open Access
D-shape optical fiber refractometer based on TM and TE lossy mode resonances
(SPIE, 2014) Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Del Villar, Ignacio; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza
The fabrication and characterization of an optical fiber refractometer based on Lossy Mode Resonances (LMR) is presented. TiO2/ poly (sodium 4-styrenesulfonate) coatings deposited on side-polished D-shaped optical fibers are used as LMR supporting coatings. LMRs are sensitive to the external medium refractive index and D-shaped optical fibers enable the observation of TE and TM LMR polarizations. These refractometers based on TE and TM LMR showed an average sensitivity of 2737 nm/RIU and 2893 nm/RIU respectively for a surrounding medium refractive index (SMRI) range from 1.35 to 1.41.
Open Access
Short single strand DNA detection by means of Lossy Mode Resonance based fiber-optic sensor
(IEEE, 2019-01-14) Zubiate Orzanco, Pablo; Ciáurriz Gortari, Paula; Tellechea Malda, Edurne; Santano Rivero, Desiree; Del Villar, Ignacio; Urrutia Azcona, Aitor; Arregui San Martín, Francisco Javier; Matías Maestro, Ignacio; Ruiz Zamarreño, Carlos; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza; Institute of Smart Cities - ISC; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, PJUPNA26
Development of DNA and RNA biosensors and the associated diagnostic tests have gained growing interest in recent years. In particular, short RNA chains (around 25 oligonucleotides), known as micro RNAs, can be associated different stroke and neurodegenerative diseases. Here, we present the utilization of highly sensitive fiber optic sensor based on Lossy Mode Resonance (LMR) for the detection of single strand complementary DNA (ssDNA) associated to Huntington disease (Hsa-miR-34b).
Open Access
Lossy mode resonance enabling ultra-low detection limit for fibre-optic biosensors (INVITED)
(Springer, 2020) Chiavaioli, Francesco; Giannetti, Ambra; Tombelli, Sara; Trono, Cosimo; Del Villar, Ignacio; Matías Maestro, Ignacio; Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Arregui San Martín, Francisco Javier; Baldini, Francesco; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Institute of Smart Cities - ISC; Ingeniería Eléctrica, Electrónica y de Comunicación; Gobierno de Navarra / Nafarroako Gobernua, 72/2015
The combination of optical fibre-based biosensors with nanotechnologies is providing the opportunity for the development of in situ, portable, lightweight, versatile and high-sensitivity optical sensing platforms. We report on the generation of lossy mode resonances (LMRs) by means of the deposition of nm-thick SnO2 film on optical fibres. This allows measuring precisely and accurately the changes in refractive index of the fibre-surrounding medium with very high sensitivity compared to other optical technology platforms, such as long period grating or surface plasmon resonance. This approach, mixed with the use of specialty fiber structures such as Dshaped fibres, allows improving the light-matter interaction in strong way. Different imaging systems, i.e. SEM and TEM along with X-EDS tool, have been used to study the optical features of the fiber coating. The shift of the LMR has been monitored in real-time thanks to conventional wavelength interrogation system and ad hoc developed microfluidics. A big leap in performance has been attained by detecting femtomolar concentrations in human serum. The biosensor reusability has been also tested by using a solution of sodium dodecyl sulphate.
Open Access
High sensitive refractometers based on lossy mode resonances (LMRs) supported by ITO coated D-shaped optical fibers
(Optical Society of America, 2015) Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Del Villar, Ignacio; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Ingeniería Eléctrica y Electrónica; Ingeniaritza Elektrikoa eta Elektronikoa
Tin doped indium oxide (ITO) coatings fabricated onto D-shaped optical fibers are presented as the supporting medium for Lossy Mode Resonances (LMRs) generation. The characteristic geometry of ITO-coated D-shaped optical fibers enables to observe experimentally LMRs obtained with both TM and TE polarized light (LMRTM and LMRTE). This permits to obtain a maximum transmission decay of 36 dB with a LMR spectral width of 6.9 nm, improving that obtained in previous works, where the LMRs were a combination of an LMRTM and an LMRTE. Surrounding medium refractive index (SMRI) sensitivity characterization of LMRTM has been performed obtaining a maximum sensitivity of 8742 nm/RIU in the range 1.365-1.38 refractive index units (RIU) which overcomes that of surface plasmon resonance-based optical fiber devices presented in recent works.
Open Access
High sensitive and selective C-reactive protein detection by means of lossy mode resonance based optical fiber devices
(Elsevier, 2017) Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Sánchez Zábal, Pedro; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Institute of Smart Cities - ISC; Ingeniería Eléctrica y Electrónica; Ingeniaritza Elektrikoa eta Elektronikoa; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
This work presents the development of high sensitive, selective, fast and reusable C-reactive protein (CRP) aptasensors. This novel approach takes advantage of the utilization of high sensitive refractometers based on Lossy Mode Resonances generated by thin indium tin oxide (ITO) films fabricated onto the planar region of D-shaped optical fibers. CRP selectivity is obtained by means of the adhesion of a CRP specific aptamer chain onto the ITO film using the Layer-by-Layer (LbL) nano-assembly fabrication process. The sensing mechanism relies on resonance wavelength shifts originated by refractive index variations of the aptamer chain in presence of the target molecule. Fabricated devices show high selectivity to CRP when compared with other target molecules, such as urea or creatinine, while maintaining a low detection limit (0.0625 mg/L) and fast response time (61 s). Additionally, these sensors show a repetitive response for several days and are reusable after a cleaning process in ultrapure water.