Jarén Ceballos, Carmen
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Jarén Ceballos
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Carmen
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IS-FOOD. Research Institute on Innovation & Sustainable Development in Food Chain
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Publication Open Access Prediction of main potato compounds by NIRS(AIDIC, 2017) López Maestresalas, Ainara; Pérez Roncal, Claudia; Tierno, Roberto; Arazuri Garín, Silvia; Ruiz de Galarreta, José Ignacio; Jarén Ceballos, Carmen; Proyectos e Ingeniería Rural; Landa Ingeniaritza eta Proiektuak; Universidad Pública de Navarra / Nafarroako Unibertsitate PublikoaPotato (Solanum tuberosum, L) compounds are generally determined by analytical methods including gasliquid chromatography (GLC), HPLC and UV-VIS spectrophotometry. These methods require a lot of time and are destructive. Therefore, they seem to be not suitable for in-line applications in the food industry. Nearinfrared spectroscopy (NIRS) is a technique that presents some advantages over reference methods for quantitative analysis of agricultural and food products since it is fast, reliable and non-destructive. For this reason, in this study, quantitative analyses were carried out to determine main compounds in potatoes using NIRS. Potato tubers grown in two consecutive years were used for the analyses. NIR spectral acquisition was acquired on lyophilized samples. In year 1, a total of 135 samples were used while 228 samples were used in year 2. Lyophilized samples were also scanned by NIRS, two replicates per samples were acquired and the mean spectrum of each sample was used for the analysis. Different chemical analyses were carried out each year. Thus, in year 1 the following parameters were quantified: reducing sugars (RS) and nitrogen (N), whereas in year 2, total soluble phenolics (TSP) and hydrophilic antioxidant capacity (HAC) were extracted and quantified. Then, chemometric analyses were performed using Unscrambler X (version 10.3, CAMO software AS, Oslo, Norway) to correlate wet chemical analysis with spectral data. Quantitative analyses based on PLS regression models were developed in order to predict the above chemical compounds of tubers in a non-destructive manner. Good PLS regression models were obtained for the prediction of nitrogen and TSP with coefficients of determination (R2) above 0.83. Moreover, PLS models obtained for the estimation of HAC could be used for screening and approximate calibrations.Publication Open Access Mapping acrylamide content in potato chips using near-infrared hyperspectral imaging and chemometrics(Elsevier, 2025-03-14) Peraza Alemán, Carlos Miguel; López Maestresalas, Ainara; Jarén Ceballos, Carmen; Ruiz de Galarreta, José Ignacio; Barandalla, Leire; Arazuri Garín, Silvia; Ingeniería; Ingeniaritza; Institute on Innovation and Sustainable Development in Food Chain - ISFOODThis study investigated the potential of near-infrared hyperspectral imaging (NIR-HSI) for the prediction of acrylamide content in potato chips. A total of 300 tubers from two potato varieties (Agria and Jaerla) grown in two seasons and processed under the same frying conditions were analysed. Partial Least Square Regression (PLSR) and Support Vector Machine Regression (SVMR), combined with a logarithmic transformation of the acrylamide levels, were applied to develop predictive models. The most optimal outcomes for PLSR yielded R2 p: 0.85, RMSEP: 201 μg/kg and RPD: 2.53, while for SVMR yielded R2 p: 0.80, RMSEP: 229 μg/kg and RPD: 2.22. Furthermore, the selection of significant wavelengths enabled an 87.95 % reduction in variables without affecting the model’s accuracy. Finally, spatial mapping of acrylamide content was conducted on all chips in the external validation set. This method provides both quantification and visualization capabilities, thus enhancing quality control for acrylamide identification in processed potatoes.Publication Open Access Applications of sensing for disease detection(Springer, 2021) Castro, Ana Isabel de; Pérez Roncal, Claudia; Thomasson, J. Alex; Ehsani, Reza; López Maestresalas, Ainara; Yang, Chenghai; Jarén Ceballos, Carmen; Wang, Tianyi; Cribben, Curtis; Marín Ederra, Diana; Isakeit, Thomas; Urrestarazu Vidart, Jorge; López Molina, Carlos; Wang, Xiwei; Nichols, Robert L.; Santesteban García, Gonzaga; Arazuri Garín, Silvia; Peña, José Manuel; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Estadística, Informática y Matemáticas; Estatistika, Informatika eta Matematika; Ingeniería; Ingeniaritza; Gobierno de Navarra / Nafarroako Gobernua; Universidad Pública de Navarra / Nafarroako Unibertsitate PublikoaThe potential loss of world crop production from the effect of pests, including weeds, animal pests, pathogens and viruses has been quantifed as around 40%. In addition to the economic threat, plant diseases could have disastrous consequences for the environment. Accurate and timely disease detection requires the use of rapid and reliable techniques capable of identifying infected plants and providing the tools required to implement precision agriculture strategies. The combination of suitable remote sensing (RS) data and advanced analysis algorithms makes it possible to develop prescription maps for precision disease control. This chapter shows some case studies on the use of remote sensing technology in some of the world’s major crops; namely cotton, avocado and grapevines. In these case studies, RS has been applied to detect disease caused by fungi using different acquisition platforms at different scales, such as leaf-level hyperspectral data and canopy-level remote imagery taken from satellites, manned airplanes or helicopter, and UAVs. The results proved that remote sensing is useful, effcient and effective for identifying cotton root rot zones in cotton felds, laurel wilt-infested avocado trees and escaaffected vines, which would allow farmers to optimize inputs and feld operations, resulting in reduced yield losses and increased profts.Publication Open Access Identificación de síntomas previsuales de salinidad mediante imágenes hiperespectrales infrarrojas en vid(Sociedad Española de Ciencias Hortícolas, 2022) Arazuri Garín, Silvia; Pérez Roncal, Claudia; Jarén Ceballos, Carmen; Santesteban García, Gonzaga; Marín Ederra, Diana; Miranda Jiménez, Carlos; López Maestresalas, Ainara; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Ingeniería; Ingeniaritza; Institute for Multidisciplinary Research in Applied Biology - IMAB; Institute on Innovation and Sustainable Development in Food Chain - ISFOODLos niveles altos de salinidad que se pueden producir en un viñedo, asociados generalmente al uso de aguas de baja calidad genera un tipo de estrés abiótico que limita la producción de la uva y afecta a la calidad de los vinos. Teniendo en cuenta la importancia de la monitorización de los cultivos en la toma de decisiones para una buena gestión del viñedo, se plantea como objetivo de este trabajo la identificación previsual de síntomas de estrés abiótico en viña por medio de la tecnología de imágenes hiperespectrales en el infrarrojo cercano (HSI-NIR). Para llevar a cabo este objetivo, se realizó un ensayo en maceta en la Finca de Prácticas de la Escuela Técnica Superior de Ingeniería Agronómica y Biociencias de la UPNA. El ensayo se realizó con plantas de un año de la variedad Monastrell sobre dos portainjertos 110R y 1103P. Se establecieron dos tratamientos: control (regado con agua de riego no salina) y salinidad (agua de riego con una concentración de sal común de 1,6 g/l). Entre finales de agosto y principios de septiembre se realizaron tres muestreos de hojas, analizando un total de 600 hojas (100 hojas/tratamiento y día). Las imágenes se tomaron con una cámara hiperespectral Xeva 1.7-320-100Hz, con rango espectral 900-1700nm. Una vez procesadas las imágenes se realizó una clasificación mediante un análisis discriminante por mínimos cuadrados parciales (PLS-DA) obteniéndose un porcentaje de muestras correctamente clasificadas en su grupo de origen (control o salinidad) del 82 % el primer día de muestreo, y del 87 % a partir del segundo día. A partir de estos datos podemos concluir que es posible identificar, mediante la tecnología HSI-NIR, síntomas en plantas sometidas a un tratamiento de riego con agua salina antes de que aparezcan síntomas en las hojas.Publication Open Access Phytochemicals determination and classification in purple and red fleshed potato tubers by analytical methods and near infrared spectroscopy(Wiley, 2016) Tierno, Roberto; López Maestresalas, Ainara; Riga, Patrick; Arazuri Garín, Silvia; Jarén Ceballos, Carmen; Ruiz de Galarreta, José Ignacio; Proyectos e Ingeniería Rural; Landa Ingeniaritza eta ProiektuakBACKGROUND: Over the last two decades, the attractive colours and shapes of pigmented tubers and the increasing concern about the relationship between nutrition and health have contributed to the expansion of their consumption and a specialty market. Thus, we have quantified the concentration of health promoting compounds such as soluble phenolics, monomeric anthocyanins, carotenoids, vitamin C, and hydrophilic antioxidant capacity, in a collection of 18 purple- and red-fleshed potato accessions. RESULTS: Cultivars and breeding lines high in vitamin C, such as Blue Congo, Morada and Kasta, have been identified. Deep purple cultivars Violet Queen, Purple Peruvian and Vitelotte showed high levels of soluble phenolics, monomeric anthocyanins, and hydrophilic antioxidant capacity, whereas relatively high carotenoid concentrations were found in partially yellow coloured tubers, such as Morada, Highland Burgundy Red, and Violet Queen. CONCLUSION: The present characterisation of cultivars and breeding lines with high concentrations of phytochemicals is an important step both to support the consideration of specialty potatoes as a source of healthy compounds, and to obtain new cultivars with positive nutritional characteristics. Moreover, by using near infrared spectroscopy a non-destructive identification and classification of samples with different levels of phytochemicals is achieved, offering an unquestionable contribution to the potato industry for future automatic discrimination of varieties.Publication Open Access Predicting the spatial distribution of reducing sugars using near-infrared hyperspectral imaging and chemometrics: a study in multiple potato genotypes(Elsevier, 2025-08-01) Peraza Alemán, Carlos Miguel; Arazuri Garín, Silvia; Jarén Ceballos, Carmen; Ruiz de Galarreta, José Ignacio; Barandalla, Leire; López Maestresalas, Ainara; Ingeniería; Ingeniaritza; Institute on Innovation and Sustainable Development in Food Chain - ISFOODThe determination of reducing sugars in potatoes is important due to their impact on product quality during industrial processing. The significant variability of these compounds between genotypes presents a challenge to the development of accurate predictive models. This study evaluated the potential of near-infrared hyperspectral imaging (NIR-HSI) for the prediction of reducing sugars in potatoes. For this, a wide range of genotypes (n=92) from two seasons (2020-2021) was selected. Partial Least Squares Regression (PLSR) and Support Vector Machine Regression (SVMR) methods were used to build the prediction models. Furthermore, interval PLS (iPLS), recursive weighted PLS (rPLS), Genetic Algorithm (GA) and Competitive Adaptive Reweighted Sampling (CARS) were used for relevant wavelength identification to develop less computationally complex models. The best full spectrum model (SNV-PLSR) achieved coefficient of determination and root mean square error values of 0.88 and 0.053% and 0.86 and 0.057%, for calibration and external validation, respectively. Variable selection algorithms successfully reduced the dimensionality of the data without compromising the performance of the models. Robust predicted models were built with only 2.65% (CARS-PLSR) and 3.57% (iPLS-SVMR) of the total wavelengths. Finally, a pixel-wise prediction was performed on the validation set and chemical images were built to visualise the spatial distribution of reducing sugars. This study demonstrated that NIR-HSI is a feasible technique for predicting reducing sugars in several potato genotypes.Publication Open Access Exploring the potential of hyperspectral imaging to detect Esca disease complex in asymptomatic grapevine leaves(Elsevier, 2022) Pérez Roncal, Claudia; Arazuri Garín, Silvia; López Molina, Carlos; Jarén Ceballos, Carmen; Santesteban García, Gonzaga; López Maestresalas, Ainara; Ingeniaritza; Estatistika, Informatika eta Matematika; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD; Ingeniería; Estadística, Informática y Matemáticas; Agronomía, Biotecnología y Alimentación; Universidad Pública de Navarra / Nafarroako Unibertsitate PublikoaPrecise and reliable identification of specific plant diseases is a challenge within precision agriculture nowadays. This is the case of esca, a complex grapevine trunk disease, that represents a major threat to modern viticulture as it is responsible for large economic losses annually. The lack of effective control strategies and the complexity of esca disease expression make essential the identification of affected plants, before symptoms become evident, for a better management of the vineyard. This study evaluated the suitability of a near-infrared hyperspectral imaging (HSI) system to detect esca disease in asymptomatic grapevine leaves of Tempranillo red-berried cultivar. For this, 72 leaves from an experimental vineyard, naturally infected with esca, were collected and scanned with a lab-scale HSI system in the 900-1700 nm spectral range. Then, effective image processing and multivariate analysis techniques were merged to develop pixel-based classification models for the distinction of healthy, asymptomatic and symptomatic leaves. Automatic and interval partial least squares variable selection methods were tested to identify the most relevant wavelengths for the detection of esca-affected vines using partial least squares discriminant analysis and different pre-processing techniques. Three-class and two-class classifiers were carried out to differentiate healthy, asymptomatic and symptomatic leaf pixels, and healthy from asymptomatic pixels, respectively. Both variable selection methods performed similarly, achieving good classification rates in the range of 82.77-97.17% in validation datasets for either three-class or two-class classifiers. The latter results demonstrated the capability of hyperspectral imaging to distinguish two groups of seemingly identical leaves (healthy and asymptomatic). These findings would ease the annual monitoring of disease incidence in the vineyard and, therefore, better crop management and decision making.Publication Open Access Early detection of Esca disease in grapevines using in-field hyperspectral proximal sensing(Hellenic Society of Agricultural Engineers, 2025) López Maestresalas, Ainara; Ruiz de Gauna González, Jon; Jarén Ceballos, Carmen; León Ecay, Sara; Arazuri Garín, Silvia; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Ingeniería; Ingeniaritza; Institute on Innovation and Sustainable Development in Food Chain - ISFOODEsca is one of the most destructive vine diseases in the world. It causes significant economic losses, mainly due to reduced grape yield and quality. Currently, the approved methods of controlling esca include preventive methods such as the use of fungicides on plant wounds or the use of planting systems that do not require intensive pruning, among others. It is therefore advisable to monitor the crop to identify those vines that are susceptible to the disease. For this reason, in this study a proximal hyperspectral camera was used for early detection of esca presence in asymptomatic grapevine leaves. Images of 11 vines of the Tempranillo variety grown in Etxauri (Navarre, Spain) were analysed. Hyperspectral images were acquired using a Specim IQ snapshot camera, mounted on a tripod, working in the range of 400¿1000 nm with a spectral resolution of 7 nm (204 bands), and an image resolution of 512 × 512 pixel including an RGB camera (5 Mpix). The images were taken under natural ambient light conditions on August 21, 2023. From the 11 vines selected, 9 showed visual symptoms of esca and the remaining 2 were asymptomatic to the naked eye. A total of 200 pixels were randomly selected from the dataset, 100 from asymptomatic leaves of asymptomatic vines (class 1) and 100 from asymptomatic leaves of symptomatic vines (class 2). Partial Least Square Discriminant Analysis (PLS-DA) was performed to classify the leaves into the two classes. Classification rates of 97% were achieved in the cross-validation dataset. Models were externally validated at pixel-level using one image of an asymptomatic vine and another of a symptomatic vine. The visualisation of the images confirmed the correct classification of the pixels into the two classes, indicating that by using proximal hyperspectral sensing an early identification of the disease is possible.Publication Open Access Siniestralidad agraria en España (2004 a 2013): factores de riesgo(Blake & Helsey, 2019) Arnal Atarés, Pedro; Jarén Ceballos, Carmen; López Maestresalas, Ainara; Mangado Ederra, Jesús; Arazuri Garín, Silvia; Ingeniería; IngeniaritzaLos datos del presente artículo son, en su mayor parte, los resultados encontrados por Pedro Arnal Atarés en el desarrollo de su tesis doctoral 'Análisis de la información sobre accidentes en el sector agrario recogida en los medios de comunicación en el decenio 2004-2013'. Esta tesis fue dirigida por Carmen Jarén Ceballos. Al objeto de conseguir el Doctorado en Prevención de Riesgos Laborales, la tesis citada se defendió el día 5 de septiembre de 2017 en la Universidad Pública de Navarra y obtuvo la calificación de Sobresaliente.Publication Open Access Hyperspectral imaging using notions from type-2 fuzzy sets(Springer, 2019) López Maestresalas, Ainara; Miguel Turullols, Laura de; López Molina, Carlos; Arazuri Garín, Silvia; Bustince Sola, Humberto; Jarén Ceballos, Carmen; Ingeniería; Ingeniaritza; Estadística, Informática y Matemáticas; Estatistika, Informatika eta Matematika; Universidad Pública de Navarra / Nafarroako Unibertsitate PublikoaFuzzy set theory has developed a prolific armamentarium of mathematical tools for each of the topics that has fallen within its scope. One of such topics is data comparison, for which a range of operators has been presented in the past. These operators can be used within the fuzzy set theory, but can also be ported to other scenarios in which data are provided in various representations. In this work, we elaborate on notions for type-2 fuzzy sets, specifically for the comparison of type-2 fuzzy membership degrees, to create function comparison operators. We further apply these operators to hyperspectral imaging, in which pixelwise data are provided as functions over a certain energy spectra. The performance of the functional comparison operators is put to the test in the context of in-laboratory hyperspectral image segmentation.