Person: Lasa Larrea, Berta
Loading...
Email Address
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
Lasa Larrea
First Name
Berta
person.page.departamento
Ciencias del Medio Natural
ORCID
0000-0001-7738-5592
person.page.upna
2202
Name
5 results
Search Results
Now showing 1 - 5 of 5
Publication Open Access Yield and quality of sugar snap pea in the Ebro Valley: sowing date and seed density(Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 2012) Azpilicueta Unanua, Miren; Irigoyen Iriarte, Ignacio; Lasa Larrea, Berta; Muro Erreguerena, Julio; Aparicio Tejo, Pedro María; Nekazaritza Ekoizpena; Natura Ingurunearen Zientziak; Producción Agraria; Ciencias del Medio Natural; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako InstitutuaSugar snap pea (Pisum sativum L. var. macrocarpon Ser.) is an edible-podded sweet pea that is being considered as a new totally mechanized crop to supply raw material to the agri-food industry of the Ebro Valley (Northern Spain). It is of great interest from an agronomic and commercial standpoint but neither its agronomic behaviour nor its adaptation to the area are known. The objective of this study was to determine the effect of sowing date and seed density on the growth and yield of the sugar snap pea at industrial scale. Six randomized blocks experiments with four replicates were conducted on irrigated land in Villafranca (Navarra, Spain) in 1998, 1999, and 2000. Three experiments for testing sowing dates (Mar., Apr., and May) and another three for seed densities (from 75 to 150 plants m–2) were performed. Phenological development, thermal integral and qualitative and quantitative yield controls were performed. Sugar snap pea required 960 ºC d–1 (Tb = 3 ºC) from sowing to harvest. The early sowings gave more biomass, but yield was similar. However, Harvest Index and crop morphology varied. The sowing densities had similar yields sowing that sugar snap pea has a bigger adaptation availability. Sugar snap peas can be satisfactorily cultivated at industrial scale in the zone with sowings between Mar. and May and with seeding densities between 75 and 150 plants m–2.Publication Open Access Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions(Public Library of Science, 2017) Rueda López, Marina; Pascual, María Belén; Pallero, Mercedes; Henao, Luisa María; Lasa Larrea, Berta; Jáuregui Mosquera, Iván; Aparicio Tejo, Pedro María; Cánovas, Francisco M.; Ávila, Concepción; Ciencias del Medio Natural; Natura Ingurunearen ZientziakIn this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However, the results in the field trial experiment diverged from those observed in the chamber system. The lines overexpressing PpDof5 showed attenuated growth during the two growing seasons and no modification of carbon or nitrogen metabolism. These results were not associated with a decrease in the expression of the transgene, but they can be ascribed to the nitrogen available in the field soil compared to that available for growth under controlled conditions. This work highlights the paramount importance of testing transgenic lines in field trials.Publication Open Access Isotopic composition of maize as related to N-fertilization and irrigation in the Mediterranean region(Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 2011) Lasa Larrea, Berta; Irañeta, Iosu; Muro Erreguerena, Julio; Irigoyen Iriarte, Ignacio; Aparicio Tejo, Pedro María; Natura Ingurunearen Zientziak; Nekazaritza Ekoizpena; Ciencias del Medio Natural; Producción Agraria; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua; Gobierno de Navarra / Nafarroako Gobernua, 17/2004Nitrate leaching as a result of excessive application of N-fertilizers and water use is a major problem of vulnerable regions. The farming of maize requires high N fertilization and water inputs in Spain. Isotopic techniques may provide information on the processes involved in the N and C cycles in farmed areas. The aim of this work was studying the impact of sprinkler and furrow irrigation and N input on maize (Zea mays L.) yields, and whether isotopic composition can be used as indicator of best farming practices. Trials were set up in Tudela (Spain) with three rates of N fertilization (0, 240 and 320 kg urea-N ha–1) and two irrigation systems (furrow and sprinkler). Yield, nitrogen content, irrigation parameters, N fate and C and N isotope composition were determined. The rate of N fertilization required to obtain the same yield is considerably higher under furrow irrigation, since the crop has less N at its disposal in furrow irrigation as a result of higher loss of nitrogen by NO3 –-N leaching and denitrification. A lower δ13C in plants under furrow irrigation was recorded.The δ15N value of plant increased with the application rate of N under furrow irrigation.Publication Open Access Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism in Arabidopsis thaliana and Brassica oleracea(Oxford University Press, 2016) Marino Bilbao, Daniel; Ariz, Idoia; Lasa Larrea, Berta; Santamaría, Enrique; Aparicio Tejo, Pedro María; Ciencias del Medio Natural; Natura Ingurunearen ZientziakAccessing different nitrogen (N) sources involves a profound adaptation of plant metabolism. In this study, a quantitative proteomic approach was used to further understand how the model plant Arabidopsis thaliana adjusts to different N sources when grown exclusively under nitrate or ammonium nutrition. Proteome data evidenced that glucosinolate metabolism was differentially regulated by the N source and that both TGG1 and TGG2 myrosinases were more abundant under ammonium nutrition, which is generally considered to be a stressful situation. Moreover, Arabidopsis plants displayed glucosinolate accumulation and induced myrosinase activity under ammonium nutrition. Interestingly, these results were also confirmed in the economically important crop broccoli (Brassica oleracea var. italica). Moreover, these metabolic changes were correlated in Arabidopsis with the differential expression of genes from the aliphatic glucosinolate metabolic pathway. This study underlines the importance of nitrogen nutrition and the potential of using ammonium as the N source in order to stimulate glucosinolate metabolism, which may have important applications not only in terms of reducing pesticide use, but also for increasing plants’ nutritional value.Publication Open Access Activity of ammonia-oxidizing bacteria in enriched cultures exposed to 3,4-dimethyl-1H-pyrazole dihydrogen phosphate nitrification inhibitor(Elsevier, 2023) Rodrigues dos Santos, Janaina Maria; Cruz, Cristina; Tenreiro, Rogerio; Gouveia, Catarina; Lasa Larrea, Berta; Institute for Multidisciplinary Research in Applied Biology - IMAB; Universidad Pública de Navarra / Nafarroako Unibertsitate PublikoaThe use of nitrification inhibitors is an interesting tool to achieve a higher N efficiency in plants while decreasing the environmental impact of N fertilization. However, an integrated evaluation of the efficiency of nitrification inhibitors over time, understood as the period in which the nitrifying activity is inhibited or slows down, is necessary to assess whether their use is ecofriendly and sustainable. To test the direct efficiency of 3,4-dimethyl-1H-pyrazole dihydrogen phosphate (DMPP) on nitrification, a study has been carried out in two cultures enriched with ammonia-oxidizing bacteria (AOB) obtained from a soil with continuous N fertilization (80 kg N ha−1 year−1 as NH4NO3) and from soil without N fertilization. In addition, Cu has been evaluated as a cofactor of ammonia monoxygenase, a key enzyme in the nitrifying activity of AOBs. On the other hand, the stability of DMP has been studied both in the cultivation system enriched in AOBs and in soil to assess the efficiency of the inhibitor due to its persistence over time. Our work reveals that nitrification rates observed in cultures enriched in AOBs from genus Nitrosospira isolated from soils with continuous N fertilization were not higher than those of cultures without N fertilization. In AOB cultures, DMPP was a very efficient inhibitor of nitrification (> 50 % inhibition of integrated AMO activity), mainly due to the stability of DMP (3,4-dimethyl-1 H-pyrazole) in the cultures. However, DMP stability was significantly lower under soil conditions (> 90 % of DMP was degraded in the first 30 days of incubation). Other metals are suggested as cofactors of the enzyme ammonia monooxygenase alternatively to Cu.