Agronomic and metabolomic side-effects of a divergent selection for indol-3-ylmethylglucosinolate content in kale (Brassica oleracea var. acephala)

Poveda Arias, Jorge; Velasco, Pablo; Haro, Antonio de; Johansen, Tor J.; McAlvay, Alex C.; Möllers, Christian; Mølmann, Jorgen A.B.; Ordiales, Elena; Rodríguez, Víctor Manuel

Publication:
Agronomic and metabolomic side-effects of a divergent selection for indol-3-ylmethylglucosinolate content in kale (Brassica oleracea var. acephala)

dc.contributor.author	Poveda Arias, Jorge
dc.contributor.author	Velasco, Pablo
dc.contributor.author	Haro, Antonio de
dc.contributor.author	Johansen, Tor J.
dc.contributor.author	McAlvay, Alex C.
dc.contributor.author	Möllers, Christian
dc.contributor.author	Mølmann, Jorgen A.B.
dc.contributor.author	Ordiales, Elena
dc.contributor.author	Rodríguez, Víctor Manuel
dc.contributor.department	Agronomía, Biotecnología y Alimentación	es_ES
dc.contributor.department	Agronomia, Bioteknologia eta Elikadura	eu
dc.date.accessioned	2022-01-11T09:29:26Z
dc.date.available	2022-01-11T09:29:26Z
dc.date.issued	2021
dc.description.abstract	Brassica oleracea var. acephala (kale) is a cruciferous vegetable widely cultivated for its leaves and flower buds in Europe and a food of global interest as a 'superfood'. Brassica crops accumulate phytochemicals called glucosinolates (GSLs) which play an important role in plant defense against biotic stresses. Studies carried out to date suggest that GSLs may have a role in the adaptation of plants to different environments, but direct evidence is lacking. We grew two kale populations divergently selected for high and low indol-3-ylmethylGSL (IM) content (H-IM and L-IM, respectively) in different environments and analyzed agronomic parameters, GSL profiles and metabolomic profile. We found a significant increase in fresh and dry foliar weight in H-IM kale populations compared to L-IM in addition to a greater accumulation of total GSLs, indole GSLs and, specifically, IM and 1-methoxyindol-3-ylmethylGSL (1MeOIM). Metabolomic analysis revealed a significant different concentration of 44 metabolites in H-IM kale populations compared to L-IM. According to tentative peak identification from MS interpretation, 80% were phenolics, including flavonoids (kaempferol, quercetin and anthocyanin derivates, including acyl flavonoids), chlorogenic acids (esters of hydroxycinnamic acids and quinic acid), hydroxycinnamic acids (ferulic acid and p-coumaric acid) and coumarins. H-IM kale populations could be more tolerant to diverse environmental conditions, possibly due to GSLs and the associated metabolites with predicted antioxidant potential.	en
dc.description.sponsorship	This research and APC was funded by the Ministry of Science and Innovation, of the Government of Spain, grant number RTI2018-096591-B-I00.	en
dc.format.extent	15 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.3390/metabo11060384
dc.identifier.issn	2218-1989
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/41697
dc.language.iso	eng	en
dc.publisher	MDPI
dc.relation.ispartof	Metabolites, 11 (6)
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096591-B-I00/ES/	en
dc.relation.publisherversion	http://doi.org/10.3390/metabo11060384
dc.rights	© 2021 by the authors. Creative Commons Attribution	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Abiotic stress	en
dc.subject	Flavonoids	en
dc.subject	Glucosinolates	en
dc.subject	Kale	en
dc.subject	Phenolics	en
dc.title	Agronomic and metabolomic side-effects of a divergent selection for indol-3-ylmethylglucosinolate content in kale (Brassica oleracea var. acephala)	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
relation.isAuthorOfPublication	85d07390-8e9d-444e-a969-87fff3f1497e
relation.isAuthorOfPublication.latestForDiscovery	85d07390-8e9d-444e-a969-87fff3f1497e