Oligomeric Bax is a component of the putative cytochrome c release channel MAC, mitochondrial apoptosis-induced channel

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dc.contributor.authorDejean, Laurent M.
dc.contributor.authorMartínez-Caballero, Sonia
dc.contributor.authorGuo, Liang
dc.contributor.authorHughes, Cynthia
dc.contributor.authorTeijido Hermida, Óscar
dc.contributor.authorDucret, Thomas
dc.contributor.authorIchas, François
dc.contributor.authorKorsmeyer, Stanley J.
dc.contributor.authorAntonsson, Bruno
dc.contributor.authorJonas, Elizabeth A.
dc.contributor.authorKinnally, Kathleen W.
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.date.accessioned2025-01-29T12:45:54Z
dc.date.available2025-01-29T12:45:54Z
dc.date.issued2005-03-16
dc.date.updated2025-01-29T12:43:13Z
dc.descriptionAcceso cerrado a este documento. No se encuentra disponible para la consulta pública. Depositado en Academica-e para cumplir con los requisitos de evaluación y acreditación académica del autor/a (sexenios, acreditaciones, etc.).es_ES
dc.description.abstractBcl-2 family proteins regulate apoptosis, in part, by controlling formation of the mitochondrial apoptosis-induced channel (MAC), which is a putative cytochrome c release channel induced early in the intrinsic apoptotic pathway. This channel activity was never observed in Bcl-2-overexpressing cells. Furthermore, MAC appears when Bax translocates to mitochondria and cytochrome c is released in cells dying by intrinsic apoptosis. Bax is a component of MAC of staurosporine-treated HeLa cells because MAC activity is immunodepleted by Bax antibodies. MAC is preferentially associated with oligomeric, not monomeric, Bax. The single channel behavior of recombinant oligomeric Bax and MAC is similar. Both channel activities are modified by cytochrome c, consistent with entrance of this protein into the pore. The mean conductance of patches of mitochondria isolated after green fluorescent protein-Bax translocation is significantly higher than those from untreated cells, consistent with onset of MAC activity. In contrast, the mean conductance of patches of mitochondria indicates MAC activity is present in apoptotic cells deficient in Bax but absent in apoptotic cells deficient in both Bax and Bak. These findings indicate Bax is a component of MAC in staurosporine-treated HeLa cells and suggest Bax and Bak are functionally redundant as components of MAC.en
dc.description.sponsorshipThis research was supported by National Institutes of Healthgrants GM-57249 (to K.W.K.) and NS-45876 (to E.A.J.) and National ScienceFoundation grants MCB-0235834 and INT003797 (to K.W.K.).
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDejean, L. M., Martinez-Caballero, S., Guo, L., Hughes, C., Teijido, O., Ducret, T., Ichas, F., Korsmeyer, S. J., Antonsson, B., Jonas, E. A., Kinnally, K. W. (2005) Oligomeric Bax is a component of the putative cytochrome c release channel MAC, mitochondrial apoptosis-induced channel. Molecular Biology of the Cell, 16(5), 2424-2432. https://doi.org/10.1091/mbc.e04-12-1111
dc.identifier.doi10.1091/mbc.e04-12-1111
dc.identifier.issn1059-1524
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/53155
dc.language.isoeng
dc.publisherAmerican Society for Cell Biology
dc.relation.ispartofMolecular Biology of the Cell, 16(5), 2424-2432
dc.relation.publisherversionhttps://doi.org/10.1091/mbc.e04-12-1111
dc.rights© 2005 by The American Society for Cell Biology
dc.rights.accessRightsinfo:eu-repo/semantics/closedAccess
dc.titleOligomeric Bax is a component of the putative cytochrome c release channel MAC, mitochondrial apoptosis-induced channelen
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublication
relation.isAuthorOfPublication2af87636-fad2-48e3-b5f6-f4a704d93613
relation.isAuthorOfPublication.latestForDiscovery2af87636-fad2-48e3-b5f6-f4a704d93613

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