Experimental polymorphism survey in intergenic regions of the icaADBCR locus in Staphylococcus aureus isolates from periprosthetic joint infections

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dc.contributor.authorMorales Laverde, Liliana Andrea
dc.contributor.authorEcheverz Sarasúa, Maite
dc.contributor.authorTrobos, Margarita
dc.contributor.authorSolano Goñi, Cristina
dc.contributor.authorLasa Uzcudun, Íñigo
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.date.accessioned2022-05-27T12:16:00Z
dc.date.available2022-05-27T12:16:00Z
dc.date.issued2022
dc.date.updated2022-05-27T12:05:07Z
dc.description.abstractStaphylococcus aureus is a leading cause of prosthetic joint infections (PJI) characterized by bacterial biofilm formation and recalcitrance to immune-mediated clearance and antibiotics. The molecular events behind PJI infection are yet to be unraveled. In this sense, identification of polymorphisms in bacterial genomes may help to establish associations between sequence variants and the ability of S. aureus to cause PJI. Here, we report an experimental nucleotide-level survey specifically aimed at the intergenic regions (IGRs) of the icaADBCR locus, which is responsible for the synthesis of the biofilm exopolysaccharide PIA/PNAG, in a collection of strains sampled from PJI and wounds. IGRs of the icaADBCR locus were highly conserved and no PJI-specific SNPs were found. Moreover, polymorphisms in these IGRs did not significantly affect transcription of the icaADBC operon under in vitro laboratory conditions. In contrast, an SNP within the icaR coding region, resulting in a V176E change in the transcriptional repressor IcaR, led to a significant increase in icaADBC operon transcription and PIA/PNAG production and a reduction in S. aureus virulence in a Galleria mellonella infection model. In conclusion, SNPs in icaADBCR IGRs of S. aureus isolates from PJI are not associated with icaADBC expression, PIA/PNAG production and adaptation to PJI.en
dc.description.sponsorshipThis work was financially supported by the Spanish Ministry of Science and Innovation grant PID2020-113494RB-I00 to I.L. (Agencia Española de Investigación/Fondo Europeo de Desarrollo Regional, European Union), the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 754412 [MoRE2020—Region Västra Götaland], and CARe—Centre for Antibiotic Resistance Research at University of Gothenburg. L.M.-L. was supported by the European Union’s H2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No 801586 (IberusTalent).en
dc.format.extent16 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMorales-Laverde, L.; Echeverz, M.; Trobos, M.; Solano, C.; Lasa, I. (2022). Experimental polymorphism survey in intergenic regions of the icaADBCR locus in Staphylococcus aureus isolates from periprosthetic joint infections. Microorganisms. 10,3 1-16en
dc.identifier.doi10.3390/microorganisms10030600
dc.identifier.issn2076-2607
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/43025
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofMicroorganisms 2022, 10, 600en
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/754412/
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/801586/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113494RB-I00/ES/
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms10030600
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPeriprosthetic joint infectionen
dc.subjectPIA/PNAGen
dc.subjectIca operonen
dc.subjectSNPsen
dc.subjectIGRsen
dc.subjectStaphylococcus aureusen
dc.titleExperimental polymorphism survey in intergenic regions of the icaADBCR locus in Staphylococcus aureus isolates from periprosthetic joint infectionsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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relation.isAuthorOfPublicationc654d104-1ae2-41cf-9215-4b4bed3e5ea6
relation.isAuthorOfPublication.latestForDiscoverydce8bd64-382c-4910-ba31-734876ed6ce8

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