Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungi

Simple item page
dc.contributor.authorRiley, Robert
dc.contributor.authorSalamov, Asaf A.
dc.contributor.authorBrown, Daren W.
dc.contributor.authorNagy, Laszlo G.
dc.contributor.authorFloudas, Dimitrios
dc.contributor.authorHeld, Benjamin W.
dc.contributor.authorLevasseur, Anthony
dc.contributor.authorLombard, Vincent
dc.contributor.authorMorin, Emmanuelle
dc.contributor.authorPisabarro de Lucas, Gerardo
dc.contributor.departmentProducción Agrariaes_ES
dc.contributor.departmentNekazaritza Ekoizpenaeu
dc.date.accessioned2018-12-28T09:14:06Z
dc.date.available2018-12-28T09:14:06Z
dc.date.issued2014
dc.description.abstractBasidiomycota (basidiomycetes) make up 32% of the described fungi and include most wood-decaying species, as well as pathogens and mutualistic symbionts. Wood-decaying basidiomycetes have typically been classified as either white rot or brown rot, based on the ability (in white rot only) to degrade lignin along with cellulose and hemicellulose. Prior genomic comparisons suggested that the two decay modes can be distinguished based on the presence or absence of ligninolytic class II peroxidases (PODs), as well as the abundance of enzymes acting directly on crystalline cellulose (reduced in brown rot). To assess the generality of the white-rot/brown-rot classification paradigm, we compared the genomes of 33 basidiomycetes, including four newly sequenced wood decayers, and performed phylogenetically informed principal-components analysis (PCA) of a broad range of gene families encoding plant biomass-degrading enzymes. The newly sequenced Botryobasidium botryosum and Jaapia argillacea genomes lack PODs but possess diverse enzymes acting on crystalline cellulose, and they group close to the model white-rot species Phanerochaete chrysosporium in the PCA. Furthermore, laboratory assays showed that both B. botryosum and J. argillacea can degrade all polymeric components of woody plant cell walls, a characteristic of white rot. We also found expansions in reducing polyketide synthase genes specific to the brown-rot fungi. Our results suggest a continuum rather than a dichotomy between the white-rot and brown-rot modes of wood decay. A more nuanced categorization of rot types is needed, based on an improved understanding of the genomics and biochemistry of wood decay.en
dc.description.sponsorshipThe work conducted by the US Department of Energy (DOE) Joint Genome Institute is supported by the Office of Science of the DOE under Contract DE-AC02-05CH11231. J.D.W. and D.J. were supported by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science Biological and Environmental Research Contract DE-FC02-07ER64494).en
dc.format.extent7 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1073/pnas.1400592111
dc.identifier.issn0027-8424 (Print)
dc.identifier.issn1091-6490 (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/31890
dc.language.isoengen
dc.publisherNational Academy of Sciencesen
dc.relation.ispartofPNAS July 8, 2014 111 (27) 9923-9928en
dc.relation.publisherversionhttps://doi.org/10.1073/pnas.1400592111
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectLignocelluloseen
dc.subjectPhylogenomicsen
dc.subjectBioenergyen
dc.titleExtensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungien
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication54daf1c4-f273-4ade-a90e-7df28e761ca7
relation.isAuthorOfPublication.latestForDiscovery54daf1c4-f273-4ade-a90e-7df28e761ca7

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
24_Riley_ExtensiveSampling.pdf
Size:
964.21 KB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
24_MatCompl.zip
Size:
893.38 KB
Format:
ZIP
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download

Collections

Artículos de revista DPA - NES Aldizkari artikuluak
Artículos de revista - Aldizkari artikuluak