Towards understanding of fungal biocontrol mechanisms of different yeasts antagonistic to Botrytis cinerea through exometabolomic analysis

Fernández San Millán, Alicia; Gamir, Jordi; Larraya Reta, Luis María; Farrán Blanch, Inmaculada; Veramendi Charola, Jon

Publication:
Towards understanding of fungal biocontrol mechanisms of different yeasts antagonistic to Botrytis cinerea through exometabolomic analysis

dc.contributor.author	Fernández San Millán, Alicia
dc.contributor.author	Gamir, Jordi
dc.contributor.author	Larraya Reta, Luis María
dc.contributor.author	Farrán Blanch, Inmaculada
dc.contributor.author	Veramendi Charola, Jon
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2023-02-06T10:46:38Z
dc.date.available	2023-02-06T10:46:38Z
dc.date.issued	2022
dc.date.updated	2023-02-06T10:00:59Z
dc.description.abstract	There is increased interest in research on yeasts as potential phytopathogen biocontrol agents due to increasing restrictions in the use of chemical pesticides. Yeast strains from a range of genera and species have been reported to inhibit postharvest decay in different fruits. However, the mechanisms behind these yeast biocontrol capacities have not been completely deciphered because they are complex and act synergistically. In this study, we performed a thorough untargeted analysis of the exometabolome generated in a co-culture of the fungal plant pathogen Botrytis cinerea with four antagonistic yeast strains: Pichia fermentans (two strains), Issatchenkia terricola and Wickerhamomyces anomalus. As a result, general and strain-specific antifungal mechanisms and molecules were identified. The P. fermentans strains secreted the highest number of differential metabolites to the extracellular medium when co-cultured with B. cinerea. In vitro antagonistic and in vivo pathogen protection assays were performed with the selected metabolites. Among a plethora of 46 differentially secreted metabolites related to yeast-fungus competitive interaction, the phenylpropanoid trans-cinnamic acid and the alkaloid indole-3-carboxaldehyde were identified as the best antagonistic metabolites against gray mold infection under in vivo protection assays. Both metabolites caused damage to the fungal membrane and increased ROS generation in spores of B. cinerea. In addition, enhanced yeast secretion to the extracellular medium of oxylipins, dipeptides, alkaloids or antibiotics deserve to be further investigated as signaling or antagonistic molecules. This study opens the door to future investigations of roles of these molecules in yeast metabolism and application of this knowledge for biotechnological purposes.	en
dc.description.sponsorship	This work was financed by the Departamento de Desarrollo Económico y Empresarial from the Gobierno de Navarra (Spain): grants 0011-1365-2021-000079 and 0011-1411-2019-000009. Open Access funding provided by Universidad Pública de Navarra.	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Millan, A. F.-S., Gamir, J., Larraya, L., Farran, I., & Veramendi, J. (2022). Towards understanding of fungal biocontrol mechanisms of different yeasts antagonistic to Botrytis cinerea through exometabolomic analysis. Biological Control, 174, 105033. https://doi.org/10.1016/j.biocontrol.2022.105033	en
dc.identifier.doi	10.1016/j.biocontrol.2022.105033
dc.identifier.issn	1049-9644
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/44667
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Biological Control 174 (2022) 105033	en
dc.relation.publisherversion	https://doi.org/10.1016/j.biocontrol.2022.105033
dc.rights	© 2022 The Authors. This is an open access article under the CC BY-NC-ND license	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Biocontrol	en
dc.subject	Botrytis	en
dc.subject	Exometabolome	en
dc.subject	Metabolomic	en
dc.subject	Pichia	en
dc.subject	Wickerhamomyces	en
dc.title	Towards understanding of fungal biocontrol mechanisms of different yeasts antagonistic to Botrytis cinerea through exometabolomic analysis	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
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