The mangotoxin biosynthetic operon (mbo) is specifically distributed within Pseudomonas syringae genomospecies 1 and was acquired only once during evolution

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dc.contributor.authorCarrión, Víctor J.
dc.contributor.authorGutiérrez Barranquero, José Antonio
dc.contributor.authorArrebola, Eva
dc.contributor.authorBardají Goikoetxea, Leire
dc.contributor.authorCodina, Juan Carlos
dc.contributor.authorVicente, Antonio de
dc.contributor.authorCazorla, Francisco M.
dc.contributor.authorMurillo Martínez, Jesús
dc.contributor.departmentProducción Agrariaes_ES
dc.contributor.departmentNekazaritza Ekoizpenaeu
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2017-03-15T18:00:12Z
dc.date.available2017-03-15T18:00:12Z
dc.date.issued2013
dc.descriptionIncluye un fichero de datoses_ES
dc.description.abstractMangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P. syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed a PCR primer pair specific for the mbo operon to examine its distribution within the P. syringae complex. These primers amplified a 692-bp DNA fragment from 52 mangotoxin-producing strains and from 7 non-mangotoxin-producing strains that harbor the mbo operon, whereas 35 non-mangotoxin-producing strains did not yield any amplification. This, together with the analysis of draft genomes, allowed the identification of the mbo operon in five pathovars (pathovars aptata, avellanae, japonica, pisi, and syringae), all of which belong to genomospecies 1, suggesting a limited distribution of the mbo genes in the P. syringae complex. Phylogenetic analyses using partial sequences from housekeeping genes differentiated three groups within genomospecies 1. All of the strains containing the mbo operon clustered in groups I and II, whereas those lacking the operon clustered in group III; however, the relative branching order of these three groups is dependent on the genes used to construct the phylogeny. The mbo operon maintains synteny and is inserted in the same genomic location, with high sequence conservation around the insertion point, for all the strains in groups I and II. These data support the idea that the mbo operon was acquired horizontally and only once by the ancestor of groups I and II from genomospecies 1 within the P. syringae complex.en
dc.description.sponsorshipThis work was supported by grants from the regional government of Andalucía (Spain), grants from CICE-Junta de Andalucía, Ayudas Grupo PAIDI AGR-169, and Proyecto de Excelencia (P07-AGR-02471) and Plan Nacional IDI grant AGL2008-55311-CO2-01 (Ministerio de Ciencia e Innovación), cofinanced by FEDER (European Union). Plan Propio of the University of Málaga funded a stay by V.J.C. at the Universidad Pública de Navarra, Spain.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1128/AEM.03007-12
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/23909
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology 2013, 79 (3)en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2008-55311/
dc.relation.publisherversionhttps://dx.doi.org/10.1128/AEM.03007-12
dc.rights© 2013 American Society for Microbiology. All Rights Reserveden
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectMangotoxinen
dc.subjectPhylogenyen
dc.subjectDiagnostic PCRen
dc.subjectAntimetabolite toxinsen
dc.subjectVirulenceen
dc.subjectMango apical necrosisen
dc.subjectLateral gene transferen
dc.titleThe mangotoxin biosynthetic operon (mbo) is specifically distributed within Pseudomonas syringae genomospecies 1 and was acquired only once during evolutionen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublicationf134625d-ae58-48ea-8e6e-b82883926409
relation.isAuthorOfPublication3f350c70-2e68-4b9b-95d6-26e9cc1540a0
relation.isAuthorOfPublication.latestForDiscoveryf134625d-ae58-48ea-8e6e-b82883926409

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