EVI1 controls proliferation in acute myeloid leukaemia through modulation of miR-1-2

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dc.contributor.authorGómez-Benito, María
dc.contributor.authorConchillo, Ana
dc.contributor.authorGarcía, M. A.
dc.contributor.authorVázquez Urio, Iria
dc.contributor.authorMaicas, Miren
dc.contributor.authorVicente, Carmen
dc.contributor.authorCristobal, I.
dc.contributor.authorMarcotegui, Nerea
dc.contributor.authorGarcía-Ortí, L.
dc.contributor.authorBandrés Elizalde, Eva
dc.contributor.authorCalasanz, María José
dc.contributor.authorAlonso, M. M.
dc.contributor.authorOdero, María D.
dc.contributor.departmentCienciases_ES
dc.contributor.departmentZientziakeu
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua
dc.date.accessioned2025-01-14T09:33:34Z
dc.date.available2025-01-14T09:33:34Z
dc.date.issued2010-09-14
dc.date.updated2025-01-14T09:20:54Z
dc.descriptionAcceso cerrado a este documento. No se encuentra disponible para la consulta pública. Depositado en Academica-e para cumplir con los requisitos de evaluación y acreditación académica del autor/a (sexenios, acreditaciones, etc.).es_ES
dc.description.abstractBakground: the EVI1(ecotropic virus integration site 1) gene codes for a zinc-finger transcription factor, whose transcriptional activation leads to a particularly aggressive form of acute myeloid leukaemia (AML). Although, EVI1 interactions with key proteins in hematopoiesis have been previously described, the precise role of this transcription factor in promoting leukaemic transformation is not completely understood. Recent works have identified specific microRNA (miRNA) signatures in different AML subgroups. However, there is no analysis of miRNAs profiles associated with EVI1 overexpression in humans. Methods: we performed QT-RT-PCR to assess the expression of 250 miRNAs in cell lines with or without EVI1 overexpression and in patient samples. We used ChIP assays to evaluated the possible binding of EVI1 binding to the putative miRNA promoter. Proliferation of the different cell lines transfected with the anti- or pre-miRs was quantified by MTT. Results: our data showed that EVI1 expression was significantly correlated with the expression of miR-1-2 and miR-133-a-1 in established cell lines and in patient samples. ChIP assays confirmed that EVI1 binds directly to the promoter of these two miRNAs. However, only miR-1-2 was involved in abnormal proliferation in EVI1 expressing cell lines. Conclusions: our data showed that EVI1 controls proliferation in AML through modulation of miR-1-2. This study contributes to further understand the transcriptional networks involving transcription factors and miRNAs in AML.en
dc.description.sponsorshipThis work was supported by Ministerio de Salud (FIS-CD07/00045), Ministerio de Educación y Ciencia (SAF2005/06425), Ministerio de Ciencia e Innovación (PI081687), ISCIII-RETIC (RD06/0020/0078 and RD06/0020/0071), Departamento Salud del Gobierno de Navarra (14/2008), and Fundació para la Investigación Médica Aplicada y UTE (Spain).
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGómez-Benito, M., Conchillo, A., García, M. A., Vázquez, I., Maicas, M., Vicente, C., Cristobal, I., Marcotegui, N., García-Ortí, L., Bandrés, E., Calasanz, M. J., Alonso, M. M., Odero, M. D. (2010) EVI1 controls proliferation in acute myeloid leukaemia through modulation of miR-1-2. British Journal of Cancer, 103(8), 1292-1296. https://doi.org/10.1038/sj.bjc.6605874
dc.identifier.doi10.1038/sj.bjc.6605874
dc.identifier.issn0007-0920
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/52911
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofBritish Journal of Cancer, 103(8), 1292-1296
dc.relation.projectIDinfo:eu-repo/grantAgreement/MSC//CD07%2F00045/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC//SAF2005//06425/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//PI081687/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MSC//RD06%2F0020%2F0071/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MSC//RD06%2F0020%2F0078/ES/
dc.relation.publisherversionhttps://doi.org/10.1038/sj.bjc.6605874
dc.rights© 2010 Cancer Research UK
dc.rights.accessRightsinfo:eu-repo/semantics/closedAccess
dc.subjectAMLen
dc.subjectEVI1en
dc.subjectMiR-1-2en
dc.subjectMiR-133a-1en
dc.titleEVI1 controls proliferation in acute myeloid leukaemia through modulation of miR-1-2en
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublication
relation.isAuthorOfPublication3762d67a-d18f-4f29-8eee-8b4c20793cd8
relation.isAuthorOfPublicationd2b52d9e-e5c2-428f-825e-91fcd49923f0
relation.isAuthorOfPublication.latestForDiscovery3762d67a-d18f-4f29-8eee-8b4c20793cd8

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