Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions

Rueda López, Marina; Pascual, María Belén; Pallero, Mercedes; Henao, Luisa María; Lasa Larrea, Berta; Jáuregui Mosquera, Iván; Aparicio Tejo, Pedro María; Cánovas, Francisco M.; Ávila, Concepción

Publication:
Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions

dc.contributor.author	Rueda López, Marina
dc.contributor.author	Pascual, María Belén
dc.contributor.author	Pallero, Mercedes
dc.contributor.author	Henao, Luisa María
dc.contributor.author	Lasa Larrea, Berta
dc.contributor.author	Jáuregui Mosquera, Iván
dc.contributor.author	Aparicio Tejo, Pedro María
dc.contributor.author	Cánovas, Francisco M.
dc.contributor.author	Ávila, Concepción
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.date.accessioned	2018-09-06T12:20:25Z
dc.date.available	2018-09-06T12:20:25Z
dc.date.issued	2017
dc.description.abstract	In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However, the results in the field trial experiment diverged from those observed in the chamber system. The lines overexpressing PpDof5 showed attenuated growth during the two growing seasons and no modification of carbon or nitrogen metabolism. These results were not associated with a decrease in the expression of the transgene, but they can be ascribed to the nitrogen available in the field soil compared to that available for growth under controlled conditions. This work highlights the paramount importance of testing transgenic lines in field trials.	en
dc.description.sponsorship	This work was supported by grants from the Spanish Ministerio de Economía y Competitividad (BIO2015-69285-R) and Junta de Andalucia (BIO-474).	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1371/journal.pone.0174748
dc.identifier.issn	1932-6203
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30562
dc.language.iso	eng	en
dc.publisher	Public Library of Science	en
dc.relation.ispartof	Plos One, 12(4): e0174748	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//BIO2015-69285-R/ES/	en
dc.relation.publisherversion	https://doi.org/10.1371/journal.pone.0174748
dc.rights	© 2017 Rueda-López et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Dof5	en
dc.subject	Hybrid poplars	en
dc.subject	Gene overexpression	en
dc.title	Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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