Role of sodium salicylate in Staphylococcus aureus quorum sensing, virulence, biofilm formation and antimicrobial susceptibility

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dc.contributor.authorTurner, Adam Benedict
dc.contributor.authorGerner, Erik
dc.contributor.authorFirdaus, Rininta
dc.contributor.authorEcheverz Sarasúa, Maite
dc.contributor.authorWerthén, María
dc.contributor.authorThomsen, Peter
dc.contributor.authorAlmqvist, Sofía
dc.contributor.authorTrobos, Margarita
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.date.accessioned2023-01-11T11:18:35Z
dc.date.available2023-01-11T11:18:35Z
dc.date.issued2022
dc.date.updated2023-01-11T10:52:16Z
dc.description.abstractThe widespread threat of antibiotic resistance requires new treatment options. Disrupting bacterial communication, quorum sensing (QS), has the potential to reduce pathogenesis by decreasing bacterial virulence. The aim of this study was to investigate the influence of sodium salicylate (NaSa) on Staphylococcus aureus QS, virulence production and biofilm formation. In S. aureus ATCC 25923 (agr III), with or without serum, NaSa (10 mM) downregulated the agr QS system and decreased the secretion levels of alpha-hemolysin, staphopain A and delta-hemolysin. Inhibition of agr expression caused a downregulation of delta-hemolysin, decreasing biofilm dispersal and increasing biofilm formation on polystyrene and titanium under static conditions. In contrast, NaSa did not increase biofilm biomass under flow but caused one log10 reduction in biofilm viability on polystyrene pegs, resulting in biofilms being twice as susceptible to rifampicin. A concentrationdependent effect of NaSa was further observed, where high concentrations (10 mM) decreased agr expression, while low concentrations (≤0.1 mM) increased agr expression. In S. aureus 8325-4 (agr I), a high concentration of NaSa (10 mM) decreased hla expression, and a low concentration of NaSa (≤1 mM) increased rnaIII and hla expression. The activity of NaSa on biofilm formation was dependent on agr type and material surface. Eight clinical strains isolated from prosthetic joint infection (PJI) or wound infection belonging to each of the four agr types were evaluated. The four PJI S. aureus strains did not change their biofilm phenotype with NaSa on the clinically relevant titanium surface. Half of the wound strains (agr III and IV) did not change the biofilm phenotype in the 3D collagen wound model. In addition, compared to the control, ATCC 25923 biofilms formed with 10 mM NaSa in the collagen model were more susceptible to silver. It is concluded that NaSa can inhibit QS in S. aureus, decreasing the levels of toxin production with certain modulation of biofilm formation. The effect on biofilm formation was dependent on the strain and material surface. It is suggested that the observed NaSa inhibition of bacterial communication is a potential alternative or adjuvant to traditional antibiotics.eu
dc.description.sponsorshipThis research was funded by the Swedish Foundation for Strategic Research (SSF; RMA15-0110 2016), Mölnlycke Health Care AB (Sweden), the European Commission within the H2020-MSCA grant agreement no. 861046 (BIOREMIA-ETN), the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement No: 754412 (MoRE2020— Region Västra Götaland), CARe—Centre for Antibiotic Resistance Research at University of Gothenburg; Swedish Research Council (2018–02891), the Swedish state under the agreement between the Swedish government and the county councils, the ALF agreement (ALFGBG-725641), the IngaBritt and Arne Lundberg Foundation (LU2021- 0048), the Hjalmar Svensson Foundation; the Doctor Felix Neuberghs Foundation, the Adlerbertska Foundation, and the Area of Advance Materials of Chalmers/GU Biomaterials within the Strategic Research Area initiative launched by the Swedish government.eu
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dc.identifier.citationTurner, A. B., Gerner, E., Firdaus, R., Echeverz, M., Werthén, M., Thomsen, P., Almqvist, S., & Trobos, M. (2022). Role of sodium salicylate in Staphylococcus aureus quorum sensing, virulence, biofilm formation and antimicrobial susceptibility. Frontiers in Microbiology, 13, 931839. https://doi.org/10.3389/fmicb.2022.931839eu
dc.identifier.doi10.3389/fmicb.2022.931839
dc.identifier.issn1664-302X
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/44552
dc.language.isoengen
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Microbiology 13:931839es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/861046/
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2022.931839es_ES
dc.rights© 2022 Turner, Gerner, Firdaus, Echeverz, Werthén, Thomsen, Almqvist and Trobos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these termses_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiofilmes_ES
dc.subjectCollagenes_ES
dc.subjectPeriprosthetic joint infectiones_ES
dc.subjectQuorum sensinges_ES
dc.subjectSodium salicylatees_ES
dc.subjectStaphylococcus aureuses_ES
dc.subjectTitaniumes_ES
dc.subjectWound infectiones_ES
dc.titleRole of sodium salicylate in Staphylococcus aureus quorum sensing, virulence, biofilm formation and antimicrobial susceptibilityes_ES
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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relation.isAuthorOfPublicationdce8bd64-382c-4910-ba31-734876ed6ce8
relation.isAuthorOfPublication.latestForDiscoverydce8bd64-382c-4910-ba31-734876ed6ce8

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