Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus
| dc.contributor.author | Alfaro Sánchez, Manuel | |
| dc.contributor.author | Majcherczyk, A. | |
| dc.contributor.author | Kües, Ursula | |
| dc.contributor.author | Ramírez Nasto, Lucía | |
| dc.contributor.author | Pisabarro de Lucas, Gerardo | |
| dc.contributor.department | Institute for Multidisciplinary Research in Applied Biology - IMAB | en |
| dc.contributor.funder | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |
| dc.date.accessioned | 2021-01-20T09:41:12Z | |
| dc.date.available | 2021-01-20T09:41:12Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources. The study revealed that this white-rot basidiomycete attacks all the components of the plant cell wall. P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source. The presence of wood increased the secretome complexity, whereas glucose diminished the secretion of enzymes involved in cellulose, hemicellulose and pectin degradation. In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes. The comparison of the secretomes of monokaryons and dikaryons reveals that secretome complexity is unrelated to the nuclear composition of the strain. | en |
| dc.description.sponsorship | Grants numbers AGL2014-55971-R (AEI, FEDER, UE) and RTI2018-099371-B-I00 (MCIU/AEI/FEDER, UE) of the Spanish National Research Plan, and PRO-UPNA18 from the Public University of Navarre have funded this research. Proteomics work in Goettingen was supported within the framework of a Common Lower Saxony-Israel-Project (ZN2043) by the Ministry of Science and Culture of Lower Saxony granted to UK and AM. MA appreciates a grant for research exchanges in Goettingen and a postdoctoral grant by Universidad Pública de Navarra. | en |
| dc.description.sponsorship | Grants numbers AGL2014-55971-R (AEI, FEDER, UE) and RTI2018-099371-B-I00 (MCIU/AEI/FEDER, UE) of the Spanish National Research Plan, and PRO-UPNA18 from the Public University of Navarre have funded this research. Proteomics work in Goettingen was supported within the framework of a Common Lower Saxony-Israel-Project (ZN2043) by the Ministry of Science and Culture of Lower Saxony granted to UK and AM. MA appreciates a grant for research exchanges in Goettingen and a postdoctoral grant by Universidad Pública de Navarra. Some of these experiments also featured in the thesis of Manuel Alfaro. | en |
| dc.format.extent | 10 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1038/s41598-020-68969-1 | |
| dc.identifier.issn | 2045-2322 (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/39025 | |
| dc.language.iso | eng | en |
| dc.publisher | Nature Research | en |
| dc.relation.ispartof | Scientific Reports, 2020, 10:12421 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2014-55971-R/ES/ | |
| dc.relation.publisherversion | https://doi.org/10.1038/s41598-020-68969-1 | |
| dc.rights | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Fungal biology | en |
| dc.subject | Proteomics | en |
| dc.title | Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
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