Publication:
Potency assessment of IFNγ-producing SARS-CoV-2- specific T cells from COVID-19 convalescent subjects

Date

2023

Authors

Gil Bescós, Rubén
Ostiz, Ainhoa
Zalba, Saioa
Rojas De Miguel, Elvira
Redondo, Margarita
Zabalza San Martín, Amaia
Ramírez, Natalia

Director

Publisher

Life Science Alliance
Acceso abierto / Sarbide irekia
Artículo / Artikulua
Versión publicada / Argitaratu den bertsioa

Project identifier

Gobierno de Navarra//0011-3597-2020-000006
Métricas Alternativas
OpenAlexGoogle Scholar
No disponible en Scopus

Abstract

The development of new therapies for COVID-19 high-risk patients remains necessary to prevent additional deaths. Here, we studied the phenotypical and functional characteristics of IFN-γ producing-SARS-CoV-2-specific T cells (SC2-STs), obtained from 12 COVID-19 convalescent donors, to determine their potency as an off-the-shelf T cell therapy product. We found that these cells present mainly an effector memory phenotype, characterized by the basal expression of cytotoxicity and activation markers, including granzyme B, perforin, CD38, and PD-1. We demonstrated that SC2-STs could be expanded and isolated in vitro, and they exhibited peptide-specific cytolytic and proliferative responses after antigenic re-challenge. Collectively, these data demonstrate that SC2-STs can be a suitable candidate for the manufacture of a T cell therapy product aimed to treat severe COVID-19.

Description

Keywords

Covid 19, SC2-STs, T cell therapy

Department

Ciencias de la Salud / Osasun Zientziak

Faculty/School

Degree

Doctorate program

item.page.cita

Gil-Bescós, R., Ostiz, A., Zalba, S., Tamayo, I., Bandrés, E., Rojas-de-Miguel, E., Redondo, M., Zabalza, A., & Ramírez, N. (2023). Potency assessment of IFNγ-producing SARS-CoV-2-specific T cells from COVID-19 convalescent subjects. Life Science Alliance, 6(6), e202201759. https://doi.org/10.26508/lsa.202201759

item.page.rights

© 2023 Gil-Bescós et al. This article is available under a Creative Commons License (Attribution 4.0 International)

Licencia

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