Genes ptz and idi, coding for cytokinin biosynthesis enzymes, are essential for tumorigenesis and in planta growth by P. syringae pv. savastanoi NCPPB 3335

Añorga García, Maite; Pintado, Adrián; Ramos, Cayo; Diego, Nuria de; Ugena, Lydia; Novák, Ondrej; Murillo Martínez, Jesús

Publication:
Genes ptz and idi, coding for cytokinin biosynthesis enzymes, are essential for tumorigenesis and in planta growth by P. syringae pv. savastanoi NCPPB 3335

dc.contributor.author	Añorga García, Maite
dc.contributor.author	Pintado, Adrián
dc.contributor.author	Ramos, Cayo
dc.contributor.author	Diego, Nuria de
dc.contributor.author	Ugena, Lydia
dc.contributor.author	Novák, Ondrej
dc.contributor.author	Murillo Martínez, Jesús
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2020-10-06T14:52:48Z
dc.date.available	2020-10-06T14:52:48Z
dc.date.issued	2020
dc.description.abstract	The phytopathogenic bacterium Pseudomonas syringae pv. savastanoi elicits aerial tumors on olive plants and is also able to synthesize large amounts of auxins and cytokinins. The auxin indoleacetic acid was shown to be required for tumorigenesis, but there is only correlational evidence suggesting a role for cytokinins. The model strain NCPPB 3335 contains two plasmid-borne genes coding for cytokinin biosynthesis enzymes: ptz, for an isopentenyl transferase and idi, for an isopentenyl-diphosphate delta-isomerase. Phylogenetic analyses showed that carriage of ptz and idi is not strictly associated with tumorigenic bacteria, that both genes were linked when first acquired by P. syringae, and that a different allele of ptz has been independently acquired by P. syringae pv. savastanoi and closely related bacteria. We generated mutant derivatives of NCPPB 3335 cured of virulence plasmids or with site-specific deletions of genes ptz and/or idi and evaluated their virulence in lignified and micropropagated olive plants. Strains lacking ptz, idi, or both produced tumors with average volumes up to 29 times smaller and reached populations up to two orders of magnitude lower than those induced by strain NCPPB 3335; these phenotypes reverted by complementation with the cloned genes. Trans-zeatin was the most abundant cytokinin in culture filtrates of NCPPB 3335. Deletion of gene ptz abolished biosynthesis of trans-zeatin and dihydrozeatin, whereas a reduced but significant amount of isopentenyladenine was still detected in the medium, suggesting the existence of other genes contributing to cytokinin biosynthesis in P. syringae. Conversely, extracts from strains lacking gene idi contained significantly higher amounts of trans-zeatin than extracts from the wild-type strain but similar amounts of the other cytokinins. This suggests that Idi might promote tumorigenesis by ensuring the biosynthesis of the most active cytokinin forms, their correct balance in planta, or by regulating the expression of other virulence genes. Therefore, gene ptz, but not gene idi, is essential for the biosynthesis of high amounts of cytokinins in culture; however, both ptz and idi are individually essential for the adequate development of tumors on olive plants by Psv NCPPB 3335.	en
dc.description.sponsorship	AP, CR, and JM were supported by grants FPU14/05551, AGL2017-82492-C2-1-R and AGL2017-82492-C2-2-R, respectively, from Ministerio de Ciencia, Innovacion y Universidades (Spain), cofinanced by the Fondo Europeo de Desarrollo Regional (FEDER). ND, LU, and ON were supported by grant CZ.02.1.01/0.0/0.0/16_019/0000827, within the program Research, Development and Education (OP RDE).	en
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.3389/fpls.2020.01294
dc.identifier.issn	1664-462X (electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/38353
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Plant Science 11:1294	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-2-R/ES/	en
dc.relation.publisherversion	https://doi.org/10.3389/fpls.2020.01294
dc.rights	© 2020 Añorga, Pintado, Ramos, De Diego, Ugena, Novák and Murillo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Pseudomonas amygdali	en
dc.subject	Pseudomonas savastanoi pv. savastanoi	en
dc.subject	Olive knot	en
dc.subject	Tumor	en
dc.subject	Pathogenicity	en
dc.subject	Virulence plasmids	en
dc.subject	Virulence genes	en
dc.subject	Indoleacetic acid	en
dc.subject	Phytohormones	en
dc.title	Genes ptz and idi, coding for cytokinin biosynthesis enzymes, are essential for tumorigenesis and in planta growth by P. syringae pv. savastanoi NCPPB 3335	en
dc.type	info:eu-repo/semantics/article
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
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relation.isAuthorOfPublication.latestForDiscovery	c282564f-36d8-4d3a-9bad-2fb078f62fb4