Lacanobia oleracea nucleopolyhedrovirus (LaolNPV): a new European species of alphabaculovirus with a narrow host range
| dc.contributor.author | Simón de Goñi, Oihane | |
| dc.contributor.author | Erlandson, Martin A. | |
| dc.contributor.author | Frayssinet, Marie | |
| dc.contributor.author | Williams, Trevor | |
| dc.contributor.author | Theilmann, David A. | |
| dc.contributor.author | Volkoff, Anne Nathalie | |
| dc.contributor.author | Caballero Murillo, Primitivo | |
| dc.contributor.department | Nekazaritza Ekoizpena | eu |
| dc.contributor.department | Producción Agraria | es_ES |
| dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es_ES |
| dc.contributor.funder | Gobierno de Navarra / Nafarroako Gobernua, IIQ1406-RI1 | es |
| dc.date.accessioned | 2018-09-06T12:20:26Z | |
| dc.date.available | 2018-09-06T12:20:26Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | During an insect sampling program in alfalfa crops near Montpellier, France in 2011, Lacanobia oleracea larvae were collected that died due to nucleopolyhedrovirus infection (LaolNPV). This virus was subjected to molecular and biological characterization. The virus was a multiple nucleocapsid NPV that showed similar restriction profiles to Mamestra configurata NPV-A (MacoNPV-A) but with significant differences. Polypeptide analysis demonstrated similar proteins in occlusion bodies and occlusion derived virions, to those observed in NPVs from Mamestra spp. Terminal sequencing revealed that the genome organization shared similarity with that of MacoNPV-A. The most homologous virus was MacoNPV-A 90/2 isolate (95.63% identity and 96.47% similarity), followed by MacoNPV-A 90/4 strain (95.37% and 96.26%), MacoNPV-B (89.21% and 93.53%) and M. brassicae MNPV (89.42% and 93.74%). Phylogenetic analysis performed with lef-8, lef-9, polh and a concatenated set of genes showed that LaolNPV and the Mamestra spp. NPVs clustered together with HaMNPV, but with a closer genetic distance to MacoNPV-A strains. The Kimura 2-parameter (K-2-P) distances of the complete genes were greater than 0.05 between LaolNPV and the MbMNPV/MacoNPV-B/HaMNPV complex, which indicates that LaolNPV is a distinct species. K-2-P distances were in the range 0.015±0.050 for comparisons of LaolNPV with MacoNPV-A strains, such that additional biological characteristics should be evaluated to determine species status. While MacoNPV-A was pathogenic to seven lepidopteran species tested, LaolNPV was only pathogenic to Chrysodeixis chalcites. Given these findings, Lacanobia oleracea nucleopolyhedrovirus should be considered as a new species in the Alphabaculovirus genus. | en |
| dc.description.sponsorship | This study received financial support from the National Institute of Agricultural Research from the Spanish Ministry project RTA2013-00114-C02-02 (Dr. Oihane Simón) and from the Department of Industry of the Government of Navarre project IIQ1406-RI1 (Dr. Primitivo Caballero). This study was supported in part by funding from Agriculture and Agri-Food Canada Research Project J000197 (Dr. Martin A. Erlandson). | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1371/journal.pone.0176171 | |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/30565 | |
| dc.language.iso | eng | en |
| dc.publisher | Public Library of Science | en |
| dc.relation.ispartof | Plos One, 12(4): e0176171 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//RTA2013-00114-C02-02/ES/ | |
| dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0176171 | |
| dc.rights | © 2017 Simón et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Lacanobia oleracea nucleopolyhedrovirus (LaolNPV) | en |
| dc.subject | Mamestra configurata NPV-A (MacoNPV-A) | en |
| dc.subject | Alphabaculovirus | en |
| dc.title | Lacanobia oleracea nucleopolyhedrovirus (LaolNPV): a new European species of alphabaculovirus with a narrow host range | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
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