NXN gene epigenetic changes in an adult neurogenesis model of Alzheimer's disease

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dc.contributor.authorBlanco Luquin, Idoia
dc.contributor.authorAcha Santamaría, Blanca
dc.contributor.authorUrdánoz Casado, Amaya
dc.contributor.authorGómez Orte, Eva
dc.contributor.authorRoldán, Miren
dc.contributor.authorPérez Rodríguez, Diego R.
dc.contributor.authorCabello, Juan
dc.contributor.authorMendióroz Iriarte, Maite
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes
dc.date.accessioned2022-07-18T10:47:13Z
dc.date.available2022-07-18T10:47:13Z
dc.date.issued2022
dc.date.updated2022-06-30T12:35:43Z
dc.description.abstractIn view of the proven link between adult hippocampal neurogenesis (AHN) and learning and memory impairment, we generated a straightforward adult neurogenesis in vitro model to recapitulate DNA methylation marks in the context of Alzheimer’s disease (AD). Neural progenitor cells (NPCs) were differentiated for 29 days and Aβ peptide 1–42 was added. mRNA expression of Neuronal Differentiation 1 (NEUROD1), Neural Cell Adhesion Molecule 1 (NCAM1), Tubulin Beta 3 Class III (TUBB3), RNA Binding Fox-1 Homolog 3 (RBFOX3), Calbindin 1 (CALB1), and Glial Fibrillary Acidic Protein (GFAP) was determined by RT-qPCR to characterize the culture and framed within the multistep process of AHN. Hippocampal DNA methylation marks previously identified in Contactin-Associated Protein 1 (CNTNAP1), SEPT5-GP1BB Readthrough (SEPT5-GP1BB), T-Box Transcription Factor 5 (TBX5), and Nucleoredoxin (NXN) genes were profiled by bisulfite pyrosequencing or bisulfite cloning sequencing; mRNA expression was also measured. NXN outlined a peak of DNA methylation overlapping type 3 neuroblasts. Aβ-treated NPCs showed transient decreases of mRNA expression for SEPT5-GP1BB and NXN on day 9 or 19 and an increase in DNA methylation on day 29 for NXN. NXN and SEPT5-GP1BB may reflect alterations detected in the brain of AD human patients, broadening our understanding of this disease.en
dc.description.sponsorshipThis research was funded by the Spanish Government through a grant from the Institute of Health Carlos III (FIS PI17/02218), jointly funded by the European Regional Development Fund (ERDF), European Union, A way of shaping Europe; the Trans-Pyrenean Biomedical Research Network (REFBIO II-MOMENEU project) and the Government of Navarra through two grants from the Department of Industry of the Government of Navarra (PI058 iBEAS-Plus and PI055 iBEAS-Plus). AUC received a grant Doctorandos industriales 2018-2020 and a Predoctoral grant (2019) founded by the Department of Industry and Health of the Government of Navarra. MM received a grant Programa de intensificación-(LCF/PR/PR15/51100006) founded by Fundación Bancaria la Caixa and Fundación Caja-Navarra, and Contrato de intensificación from the Institute of Health Carlos III (INT19/00029).en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.citationBlanco-Luquin, I.; Acha-Santamaría, B.; Urdánoz-Casado, A.; Gómez-Orte, E.; Roldan, M.; Pérez-Rodríguez, D. R.; Cabello, J.; Mendioroz, M. (2022). NXN gene epigenetic changes in an adult neurogenesis model of alzheimer's disease. Cells. 11,7en
dc.identifier.doi10.3390/cells11071069
dc.identifier.issn2073-4409
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/43329
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofCells, 2022, 11 (7), p. 1069en
dc.relation.publisherversionhttps://doi.org/10.3390/cells11071069
dc.rights© 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) licenseen
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAdult hippocampal neurogenesisen
dc.subjectNPCsen
dc.subjectAlzheimer’s diseaseen
dc.subjectAβ peptideen
dc.subjectDNA methylationen
dc.subjectGene expressionen
dc.subjectNXNen
dc.subjectCNTNAP1en
dc.subjectSEPT5-GP1BBen
dc.subjectTBX5en
dc.titleNXN gene epigenetic changes in an adult neurogenesis model of Alzheimer's diseaseen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublicationd09deda5-8d94-42e0-97f0-b32e34571988
relation.isAuthorOfPublicationc0cb79d9-6664-4b47-bcd1-b7cb133d224e
relation.isAuthorOfPublication.latestForDiscoveryd09deda5-8d94-42e0-97f0-b32e34571988

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