Elevated CO2 improved the growth of a double nitrate reductase defective mutant of Arabidopsis thaliana: the importance of maintaining a high energy status

Jáuregui Mosquera, Iván; Aparicio Tejo, Pedro María; Baroja Fernández, Edurne; Ávila, Concepción; Aranjuelo Michelena, Iker

Publication:
Elevated CO2 improved the growth of a double nitrate reductase defective mutant of Arabidopsis thaliana: the importance of maintaining a high energy status

dc.contributor.author	Jáuregui Mosquera, Iván
dc.contributor.author	Aparicio Tejo, Pedro María
dc.contributor.author	Baroja Fernández, Edurne
dc.contributor.author	Ávila, Concepción	es_ES
dc.contributor.author	Aranjuelo Michelena, Iker
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2020-10-14T10:56:01Z
dc.date.available	2020-10-14T10:56:01Z
dc.date.issued	2017
dc.description.abstract	Impairments in leaf nitrogen (N) assimilation in C3 plants have been identified as processes conditioning photosynthesis under elevated [CO2], especially when N is supplied as nitrate. Leaf N status is usually improved under ammonium nutrition and elevated [CO2]. However, ammonium fertilization is usually accompanied by the appearance of oxidative stress symptoms, which constrains plant development. To understand how the limitations of direct fertilization with ammonium (growth reduction attributed to ammonium toxicity) can be overcome, the effects of elevated [CO2] (800 ppm) exposure were studied in the Arabidopsis thaliana double nitrate reductase defective mutant, nia1-1/chl3-5 (which preferentially assimilates ammonium as its nitrogen source). Analysis of the physiology, metabolites and gene expression was carried out in roots and shoot organs. Our study clearly showed that elevated [CO2] improved the inhibited phenotype of the nitrate reductase double mutant. Both the photosynthetic rates and the leaf N content of the NR mutant under elevated CO2 were similar to wild type plants. The growth of the nitrate reductase mutant was linked to its ability to overcome ammonium-associated photoinhibition processes at 800 ppm [CO2]. More specifically: (i) the capacity of NR mutants to equilibrate energy availability, as reflected by the electron transport equilibrium reached (photosynthesis, photorespiration and respiration), (ii) as well as by the upregulation of genes involved in stress tolerance were identified as the processes involved in the improved performance of NR mutants.	en
dc.description.sponsorship	This work has been funded by the Spanish National Research and Development Programme (AGL2009-13339-C02-02, AGL2011-30386-C02-02 and AGL2012-37815-C05-05). Ivan Jauregui was the holder of a FPI fellowship from the Spanish Ministry of Economy and Competitiveness.	en
dc.format.extent	41 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1016/j.envexpbot.2017.06.003
dc.identifier.issn	0098-8472
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/38385
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Environmental and Experimental Botany, 2017, 140, 110-119	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-30386-C02-01/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2012-37815-C05-05/ES/	en
dc.relation.publisherversion	https://doi.org/10.1016/j.envexpbot.2017.06.003
dc.rights	© 2017 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	[CO2]	en
dc.subject	Plant physiology	en
dc.subject	Nitrate reductase	en
dc.subject	Ammonium	en
dc.subject	Photosynthesis	en
dc.subject	Root-to-shoot	en
dc.subject	Transcriptomics	en
dc.subject	Redox	en
dc.title	Elevated CO2 improved the growth of a double nitrate reductase defective mutant of Arabidopsis thaliana: the importance of maintaining a high energy status	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dspace.entity.type	Publication
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