Stability of a Spodoptera frugiperda nucleopolyhedrovirus deletion recombinant during serial passage in insects

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dc.contributor.authorSimón de Goñi, Oihane
dc.contributor.authorWilliams, Trevor
dc.contributor.authorPossee, Robert D.
dc.contributor.authorLópez Ferber, Miguel
dc.contributor.authorCaballero Murillo, Primitivo
dc.contributor.departmentNekazaritza Ekoizpenaeu
dc.contributor.departmentProducción Agrariaes_ES
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2019-01-10T08:27:55Z
dc.date.available2019-01-10T08:27:55Z
dc.date.issued2009
dc.description.abstractThe stabilities of the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) complete genome bacmid (Sfbac) and a deletion recombinant (Sf29null) in which the Sf29 gene was replaced by a kanamycin resistance cassette were determined during sequential rounds of per os infection in insect larvae. The Sf29 gene is a viral factor that determines the number of virions in occlusion bodies (OBs). The Sf29null bacmid virus was able to recover the Sf29 gene during passage. After the third passage (P3) of Sf29null bacmid OBs, the population was observed to reach an equilibrium involving a mixture of those with a kanamycin resistance cassette and those with the Sf29 gene. The biological activity of Sf29null bacmid OBs at P3 was similar to that of Sfbac OBs. The recovered gene in the Sf29null virus was 98 to 100% homologous to the Sf29 genes of different SfMNPV genotypes. Reverse transcription-PCR analysis of uninoculated S. frugiperda larvae confirmed the expression of the SfMNPV ie-0 and Sf29 genes, indicating that the insect colony harbors a covert SfMNPV infection. Additionally, the nonessential bacterial artificial chromosome vector was spontaneously deleted from both viral genomes upon passage in insects.en
dc.description.sponsorshipThis study received financial support from the Spanish Ministry for Science and Technology projects AGL2005-07909-CO3-01 and AGL2008-05456-CO3-01/AGR. O.S. received a Spanish Ministry of Education and Culture postdoctoral fellowship.en
dc.format.extent7 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1128/aem.01894-09
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/31939
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, Feb. 2010, Vol. 76, No. 3, p. 803–809en
dc.relation.publisherversionhttps://doi.org/10.1128/aem.01894-09
dc.rights© 2010, American Society for Microbiology. All Rights Reserved.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectSpodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)en
dc.subjectSerial passageen
dc.titleStability of a Spodoptera frugiperda nucleopolyhedrovirus deletion recombinant during serial passage in insectsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication5b9012ab-c911-4ef7-9a92-0041bc07bc6f
relation.isAuthorOfPublication0b5a3470-9d73-4ac7-a923-c4f6079df358
relation.isAuthorOfPublicationecde8e03-14c2-46a3-9e89-ae3b2c668297
relation.isAuthorOfPublication.latestForDiscovery5b9012ab-c911-4ef7-9a92-0041bc07bc6f

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