Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity

Castanera Andrés, Raúl; Omarini, Alejandra; Santoyo Santos, Francisco; Pérez Garrido, María Gumersinda; Pisabarro de Lucas, Gerardo; Ramírez Nasto, Lucía

Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity

dc.contributor.author	Castanera Andrés, Raúl
dc.contributor.author	Omarini, Alejandra
dc.contributor.author	Santoyo Santos, Francisco
dc.contributor.author	Pérez Garrido, María Gumersinda
dc.contributor.author	Pisabarro de Lucas, Gerardo
dc.contributor.author	Ramírez Nasto, Lucía
dc.contributor.department	Producción Agraria	es_ES
dc.contributor.department	Nekazaritza Ekoizpena	eu
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2018-09-06T12:20:21Z
dc.date.available	2018-09-06T12:20:21Z
dc.date.issued	2013
dc.description.abstract	Background: The basidiomycete Pleurotus ostreatus is an efficient producer of laccases, a group of enzymes appreciated for their use in multiple industrial processes. The aim of this study was to reveal the molecular basis of the superiority of laccase production by dikaryotic strains compared to their parental monokaryons. Methodology/Principal Findings: We bred and studied a set of dikaryotic strains starting from a meiotic population of monokaryons. We then completely characterised the laccase allelic composition, the laccase gene expression and activity profiles in the dikaryotic strain N001, in two of its meiotic full-sib monokaryons and in the dikaryon formed from their mating. Conclusions/Significance: Our results suggested that the dikaryotic superiority observed in laccase activity was due to nonadditive transcriptional increases in lacc6 and lacc10 genes. Furthermore, the expression of these genes was divergent in glucose- vs. lignocellulose-supplemented media and was highly correlated to the detected extracellular laccase activity. Moreover, the expression profile of lacc2 in the dikaryotic strains was affected by its allelic composition, indicating a putative single locus heterozygous advantage.	en
dc.description.sponsorship	This work was supported by funds obtained from AGL2011-30495 of the Spanish National Research Plan; Bioethanol-Euroinnova, the Autonomous Government of Navarre; and by additional institutional support from the Public University of Navarre.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1371/journal.pone.0073282
dc.identifier.issn	1932-6203
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30551
dc.language.iso	eng	en
dc.publisher	Public Library of Science	en
dc.relation.ispartof	Plos One, 8(9): e73282	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-30495/ES/
dc.relation.publisherversion	https://doi.org/10.1371/journal.pone.0073282
dc.rights	© 2013 Castanera et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/
dc.subject	Pleurotus ostreatus	en
dc.subject	Laccases	en
dc.subject	Dikaryotic strains	en
dc.title	Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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