Induction of the PDH bypass and upregulation of the ALDH7B4 in plants treated with herbicides inhibiting amino acid biosynthesis

Gil Monreal, Miriam; Zabalza Aznárez, Ana; Missihoun, Tagnon D.; Dormann, Peter; Bartels, Dorothea

Publication:
Induction of the PDH bypass and upregulation of the ALDH7B4 in plants treated with herbicides inhibiting amino acid biosynthesis

dc.contributor.author	Gil Monreal, Miriam
dc.contributor.author	Zabalza Aznárez, Ana
dc.contributor.author	Missihoun, Tagnon D.	es_ES
dc.contributor.author	Dormann, Peter	es_ES
dc.contributor.author	Bartels, Dorothea	es_ES
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2017-09-13T07:33:49Z
dc.date.available	2019-11-01T00:00:11Z
dc.date.issued	2017
dc.description	Incluye 3 ficheros de datos	es_ES
dc.description.abstract	Imazamox and glyphosate represent two classes of herbicides that inhibit the activity of acetohydroxyacid synthase in the branched-chain amino acid biosynthesis pathway and the activity of 5-enolpyruvylshikimate-3-phosphate synthase in the aromatic amino acid biosynthesis pathway, respectively. However, it is still unclear how imazamox and glyphosate lead to plant death. Both herbicides inhibit amino-acid biosynthesis and were found to induce ethanol fermentation in plants, but an Arabidopsis mutant deficient in alcohol dehydrogenase 1 was neither more susceptible nor more resistant than the wild-type to the herbicides. In this study, we investigated the effects of the amino acid biosynthesis inhibitors, imazamox and glyphosate, on the pyruvate dehydrogenase bypass reaction and fatty acid metabolism in A. thaliana. We found that the pyruvate dehydrogenase bypass was upregulated following the treatment by the two herbicides. Our results suggest that the Arabidopsis aldehyde dehydrogenase 7B4 gene might be participating in the pyruvate dehydrogenase bypass reaction. We evaluated the potential role of the aldehyde dehydrogenase 7B4 upon herbicide treatment in the plant defence mechanism. Plants that overexpressed the ALDH7B4 gene accumulated less soluble sugars, starch, and fatty acids and grew better than the wild-type after herbicide treatment. We discuss how the upregulation of the ALDH7B4 alleviates the effects of the herbicides, potentially through the detoxification of the metabolites produced in the pyruvate dehydrogenase bypass.	en
dc.description.sponsorship	Miriam Gil-Monreal received funding from fellowships through Universidad Pública de Navarra. This work was financially supported by two grants from the Ministerio Español de Economía y Competitividad (AGL-2013-40567R and AGL-2016-77531R).	en
dc.embargo.lift	2019-11-01
dc.embargo.terms	2019-11-01
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1016/j.plantsci.2017.08.003
dc.identifier.issn	0168-9452 (Print)
dc.identifier.issn	1873-2259 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/25561
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Plant Science 264 (2017) 16-28	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2013-40567-R/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/ES/1PE/AGL2016-77531	en
dc.relation.publisherversion	https://doi.org/10.1016/j.plantsci.2017.08.003
dc.rights	© 2017 Elsevier B.V. The manuscript version is made available under the CC BY-NC-ND 4.0 license.	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Acetohydroxyacid synthase	en
dc.subject	5-enolpyruvylshikimate-3-phosphate synthase	en
dc.subject	Ethanol fermentation	en
dc.subject	Fatty acids	en
dc.subject	Glyphosate	en
dc.subject	Imazamox	en
dc.title	Induction of the PDH bypass and upregulation of the ALDH7B4 in plants treated with herbicides inhibiting amino acid biosynthesis	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dspace.entity.type	Publication
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relation.isAuthorOfPublication.latestForDiscovery	b62dd25b-6298-44fe-8690-12267f5b3d70