Deciphering the function of Com_YlbF domain-containing proteins in Staphylococcus aureus

Simple item page
dc.contributor.authorCorredor Rozo, Zayda Lorena
dc.contributor.authorMarquez-Ortiz, Ricaurte Alejandro
dc.contributor.authorVelandia-Romero, Myriam Lucia
dc.contributor.authorAbril Riaño, Deisy Julieth
dc.contributor.authorMadroñero, Leidy Johana
dc.contributor.authorPrada, Luisa F.
dc.contributor.authorVanegas-Gomez, Natasha
dc.contributor.authorGarcía Martínez, Begoña
dc.contributor.authorEcheverz Sarasúa, Maite
dc.contributor.authorCalderón-Peláez, María Angelica
dc.contributor.authorChaparro-Olaya, Jacqueline
dc.contributor.authorMorales de la Pava, Liliana
dc.contributor.authorNieto-Clavijo, Carlos
dc.contributor.authorEscobar-Pérez, Javier
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.date.accessioned2025-09-19T11:37:17Z
dc.date.available2025-09-19T11:37:17Z
dc.date.issued2025
dc.date.updated2025-09-19T11:23:49Z
dc.description.abstractThe Com_YlbF domain-containing proteins inhibit sporulation, competition, and biofilm formation by affecting the activity of ribonuclease RNase-Y in Bacillus subtilis. Similar Com_YlbF proteins are found in Staphylococcus aureus, but their function is yet to be determined. This study investigates the role of Com_YlbF domain-containing proteins (Qrp/YheA, YmcA, and YlbF) in S. aureus by evaluating the impact of qrp/yheA, ymcA, and ylbF gene deletion on biofilm formation, PIA/PNAG production, and hemolytic capacity. It was found that deletion of the three qrp/yheA, ymcA, and ylbF genes in S. aureus produced a decrease in biofilm formation, a slight decrease in PIA/PNAG production, and a decrease in its hemolytic capacity. Global transcriptional analysis in the mutant strain showed dysregulation of several genes associated with pathogenesis, most notably a decreased expression of the agrA gene (quorum sensing system), the delta, alpha, and gamma hemolysins, as well as the sdrC genes and eap/map (adhesion proteins) genes. It also showed an attenuation of virulence, manifesting as enhanced survival among Galleria mellonella larvae and BALB/c mice, possibly associated due to disrupted RNase-Y activity when the qrp/yheA, ymcA, and ylbF are deleted. In conclusion, this work supports the hypothesis that this new family of proteins containing a Com_YlbF domain is involved in the regulation of genes related to biofilm formation, hemolysis, and virulence in S. aureus through a change in RNase-Y activity.en
dc.description.sponsorshipThis work was supported by Ministerio de Ciencia, Tecnología e Innovación of Colombia-MinCiencias (grant number 910-2021) and Universidad El Bosque (grant numbers 2018-216, 2018-10227, and 2022_11031). The funders had no role in the study of design, data collection, and interpretation, or the decision to submit the work for publication.
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/zip
dc.identifier.citationCorredor-Rozo, Z. L., Marquez-Ortiz, R., Velandia-Romero, M. L., Abril, D., Madroñero, J., Prada, L. F., Vanegas-Gomez, N., García, B., Echeverz, M., Calderón-Peláez, M. A., Chaparro-Olaya, J., Morales, L., Nieto-Clavijo, C., Escobar-Pérez, J. (2025). Deciphering the function of Com_YlbF domain-containing proteins in Staphylococcus aureus. Journal of Bacteriology, 207(9), 1-18. https://doi.org/10.1128/jb.00061-25.
dc.identifier.doi10.1128/jb.00061-25
dc.identifier.issn0021-9193
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/55060
dc.language.isospa
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofJournal of Bacteriology (2025), vol. 27, núm. 9, e00061-25
dc.relation.publisherversionhttps://doi.org/10.1128/jb.00061-25
dc.rights© 2025 Corredor-Rozo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectStaphylococcus aureusen
dc.subjectCom_YlbF domain proteinsen
dc.subjectBiofilmen
dc.subjectVirulenceen
dc.subjectRNase-Yen
dc.titleDeciphering the function of Com_YlbF domain-containing proteins in Staphylococcus aureusen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication7f89244d-67ab-4c4d-b868-ad7ae9112055
relation.isAuthorOfPublicationdce8bd64-382c-4910-ba31-734876ed6ce8
relation.isAuthorOfPublication.latestForDiscoverydce8bd64-382c-4910-ba31-734876ed6ce8

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Corredor_Deciphering.pdf
Size:
5.22 MB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
Corredor_DecipheringFunction_MatCompl.zip
Size:
1.16 MB
Format:
Unknown data format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download

Collections

Artículos de revista DCS - OZS Aldizkari artikuluak
Artículos de revista - Aldizkari artikuluak