The moderate oxidative stress induced by glyphosate is not detected in Amaranthus palmeri plants overexpressing EPSPS

Gil Monreal, Miriam; Barco Antoñanzas, María; Zabalza Aznárez, Ana; Royuela Hernando, Mercedes; Eceiza, Mikel Vicente

The moderate oxidative stress induced by glyphosate is not detected in Amaranthus palmeri plants overexpressing EPSPS

dc.contributor.author	Gil Monreal, Miriam
dc.contributor.author	Barco Antoñanzas, María
dc.contributor.author	Zabalza Aznárez, Ana
dc.contributor.author	Royuela Hernando, Mercedes
dc.contributor.author	Eceiza, Mikel Vicente
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2022-09-05T07:51:42Z
dc.date.available	2022-09-05T07:51:42Z
dc.date.issued	2022
dc.date.updated	2022-09-05T07:42:36Z
dc.description.abstract	The present study aimed to determine whether glyphosate-induced oxidative stress is directly related to the action mechanism of this herbicide (5-enolpyruvylshikimate-3-phosphate synthase or EPSPS inhibition) and analyse the role of oxidative stress in glyphosate toxicity of the weed Amaranthus palmeri S. Wats. Two kinds of populations were studied using EPSPS amplification: glyphosate-sensitive and glyphosate-resistant (by gene amplification). Plants were grown hydroponically and treated with different glyphosate doses, after which several oxidative stress markers were measured in the leaves. Untreated, sensitive and resistant plants showed similar values for the analysed parameters. Treated glyphosate-sensitive plants showed an increase in shikimate, superoxide and H2O2 contents and dose-dependent lipid peroxidation and antioxidant responses; however, none of these effects were observed in resistant plants, indicating that glyphosate-induced oxidative stress is related to EPSPS inhibition. Oxidative stress is associated with an increase in the activity of peroxidases due to EPSPS inhibition, although the link between both processes remains elusive. The fact that some glyphosate doses were lethal but did not induce major oxidative damage provides evidence that glyphosate toxicity is independent of oxidative stress.	en
dc.description.sponsorship	This work was funded by the Spanish Ministry of Economy and Competitiveness (AGL2016-77531-R), the Public University of Navarre (Project UPNA20-6138) and the Spanish Ministry of Science and Innovation (2020-117723RB-100). M.V. Eceiza is the holder of a predoctoral fellowship of the Basque Government. Open access funding provided by Universidad Pública de Navarra.	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Eceiza, M. V.; Gil-Monreal, M.; Barco-Antoñanzas, M.; Zabalza, A.; Royuela, M.. (2022). The moderate oxidative stress induced by glyphosate is not detected in Amaranthus palmeri plants overexpressing EPSPS. Journal of Plant Physiology. 274, 1-29 .	en
dc.identifier.doi	10.1016/j.jplph.2022.153720
dc.identifier.issn	0176-1617
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/43969
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Journal of Plant Physiology 274 (2022) 153720	es_ES
dc.relation.projectID	info:eu-repo/grantAgreement/ES/1PE/AGL2016-77531-R/
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117723RB-I00/ES/
dc.relation.publisherversion	https://doi.org/10.1016/j.jplph.2022.153720
dc.rights	© 2022 The Authors. This is an open access article under the CC BY license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Glyphosate	en
dc.subject	Herbicide mode of action	en
dc.subject	Oxidative Stress	en
dc.title	The moderate oxidative stress induced by glyphosate is not detected in Amaranthus palmeri plants overexpressing EPSPS	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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