QTLs identification for iron chlorosis in a segregating peach-almond progeny through double-digest sequence-based genotyping (SBG)

Guajardo, Verónica; Martínez García, Pedro José; Solís, Simón; Calleja Satrustegui, Aitziber; Saski, Christopher; Moreno, María Ángeles

QTLs identification for iron chlorosis in a segregating peach-almond progeny through double-digest sequence-based genotyping (SBG)

dc.contributor.author	Guajardo, Verónica
dc.contributor.author	Martínez García, Pedro José
dc.contributor.author	Solís, Simón
dc.contributor.author	Calleja Satrustegui, Aitziber
dc.contributor.author	Saski, Christopher
dc.contributor.author	Moreno, María Ángeles
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2023-01-17T13:26:40Z
dc.date.available	2023-01-17T13:26:40Z
dc.date.issued	2022
dc.date.updated	2023-01-17T13:13:42Z
dc.description.abstract	Linkage maps are highly appreciated tools for cultivar and rootstock breeding programs because they are suitable for genetic and genomic studies. In this study, we report on using sequence-based genotyping (SBG) approach to simultaneously discover and genotype SNPs from two peach-based rootstocks ("Adafuel" and "Flordaguard") and their progeny (n = 118): from a initial mapping population composed of 131 seedlings. The plant material was developed at the EEAD-CSIC Prunus rootstocks breeding program, aiming to obtain a segregating progeny for a range of characters of agronomical interest to rootstock breeding (iron-chlorosis and root-asphyxia tolerance, nematode resistance, vigor traits, and other effects on scion cultivars). Sequence reads obtained from double-digest SBG were aligned to the P. persica reference genome (Peach v2.0). While eight linkage groups were constructed for "Adafuel", only four linkage groups were constructed for "Flordaguard", given the low heterozygosity of this last genotype. High synteny and co-linearity were observed between obtained maps and Peach v2.0. On the other hand, this work aimed to elucidate the genetic basis of leaf chlorosis tolerance using the phenotypic segregation of the progeny to iron-chlorosis tolerance, along with the QTLs responsible for leaf chlorosis. The F1 mapping population, composed initially of 131 seedlings, was growing in four field trials established on calcareous soils at the experimental field of the EEAD-CSIC in Zaragoza, Spain. From the initial mapping population, 131 individuals were selected for their phenotypical characterization with SPAD measurements of plants grown in the field, exhibiting a great variability. Significant QTLs associated with tolerance to iron chlorosis were found in LG1, LG5, LG7, and LG8. The significant QTLs detected in LG5 and LG7 have not been associated with this abiotic stress before in Prunus. Several candidate genes such as Prupe.1G541100, predicted as glutamyl-tRNA reductase 1, Prupe.1G468200, encoding a 2-oxoglutarate (2OG), and Fe(II)-dependent oxygenase superfamily protein or Prupe.1G577000 (ppa011050.m), a NIFU-like protein 2 (NIFU2) were detected. The exact biological function of some of these genes should be verified for the future development of marker-assisted selection for peach iron chlorosis tolerance.	en
dc.description.sponsorship	This research was funded by the Spanish Ministry of Science, Innovation and Universities (MICINN) grants RFP2015-00019 and RTI2018-094176-R-C33, co-funded by ERDF A way of making EUROPE and the Regional Government of Aragon (A44 and T07-17R). It was also funded by CONICYT-REGIONAL/GORE O'HIGGINS/CEAF/R19A10003, FONDECYT 3160316, and CONICYT R16F20006 from Chile.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.citation	Guajardo, V., Martínez-García, P. J., Solís, S., Calleja-Satrustegui, A., Saski, C., & Moreno, M. Á. (2022). Qtls identification for iron chlorosis in a segregating peach–almond progeny through double-digest sequence-based genotyping(Sbg). Frontiers in Plant Science, 13, 872208. https://doi.org/10.3389/fpls.2022.872208	en
dc.identifier.doi	10.3389/fpls.2022.872208
dc.identifier.issn	1664-462X
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/44564
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Plant Science 2022, 13 (872208)	en
dc.relation.publisherversion	https://doi.org/10.3389/fpls.2022.872208
dc.rights	© 2022 Guajardo, Martínez-García, Solís, Calleja-Satrustegui, Saski and Moreno. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Molecular markers	en
dc.subject	Pseudomolecule	en
dc.subject	Reference genome	en
dc.subject	Rootstock	en
dc.subject	Segregating population	en
dc.subject	SPAD	en
dc.subject	Stone fruits	en
dc.title	QTLs identification for iron chlorosis in a segregating peach-almond progeny through double-digest sequence-based genotyping (SBG)	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
relation.isAuthorOfPublication	79189302-94db-4c2e-a635-67918f35689e
relation.isAuthorOfPublication.latestForDiscovery	79189302-94db-4c2e-a635-67918f35689e