Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma

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dc.contributor.authorValencia, Karmele
dc.contributor.authorSáinz, Cristina
dc.contributor.authorBértolo, Cristina
dc.contributor.authorBiurrun, Gabriel de
dc.contributor.authorAgorreta Arrazubi, Jackeline
dc.contributor.authorAzpilikueta, Arantza
dc.contributor.authorLarrayoz, Marta
dc.contributor.authorBosco, Graziella
dc.contributor.authorZandueta, Carolina
dc.contributor.authorRedrado, Miriam
dc.contributor.authorRedin, Esther
dc.contributor.authorExpósito, Francisco
dc.contributor.authorSerrano, Diego
dc.contributor.authorEchepare, Mirari
dc.contributor.authorAjona, Daniel
dc.contributor.authorMelero, Ignacio
dc.contributor.authorPío, Rubén
dc.contributor.authorThomas, Roman
dc.contributor.authorCalvo, Alfonso
dc.contributor.authorMontuenga, Luis M.
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.date.accessioned2022-04-12T08:44:51Z
dc.date.available2022-04-12T08:44:51Z
dc.date.issued2022
dc.description.abstractThere is a paucity of adequate mouse models and cell lines available to study lung squamous cell carcinoma (LUSC). We have generated and characterized two models of phenotypically different transplantable LUSC cell lines, i.e. UN-SCC679 and UN-SCC680, derived from A/J mice that had been chemically induced with N-nitroso-tris-chloroethylurea (NTCU). Furthermore, we genetically characterized and compared both LUSC cell lines by performing whole-exome and RNA sequencing. These experiments revealed similar genetic and transcriptomic patterns that may correspond to the classic LUSC human subtype. In addition, we compared the immune landscape generated by both tumor cells lines in vivo and assessed their response to immune checkpoint inhibition. The differences between the two cell lines are a good model for the remarkable heterogeneity of human squamous cell carcinoma. Study of the metastatic potential of these models revealed that both cell lines represent the organotropism of LUSC in humans, i.e. affinity to the brain, bones, liver and adrenal glands. In summary, we have generated valuable cell line tools for LUSC research, which recapitulates the complexity of the human disease.en
dc.description.sponsorshipThis work was supported by FIMA, Centro de Investigacion Biomedica en Red de Cancer (CIBERONC) (grant number: CB16/12/00443), Fundacion Cientifica Asociacion Espanola Contra el Cancer (grant number: GCB14-2170), Fundacion Ramon Areces, Instituto de Salud Carlos III and the European Regional Development Fund (ERDF, A way to make Europe) (grant numbers: PI19/00098; PI19/00230; PI20/00419), Fundacion Roberto Arnal Planelles and an IASLC Fellowship funding (K.V.); D.S. was supported by the Juan de la Cierva-Incorporacion program, Spanish Ministry of Science and Innovation (grant number: IJCI-2016-27595); E.R. was supported by a FPU, Spanish Ministry of Education ( grant number: FPU17/01168); M.E. was supported by PFIS, Spanish Ministry of Health, M.L. was supported by a Junior Investigator grant from AECC.en
dc.format.extent41 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1242/dmm.049137
dc.identifier.issn1754-8403
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/42741
dc.language.isoengen
dc.publisherThe Company of Biologistsen
dc.relation.ispartofDisease Models & Mechanisms, (2022) 15, dmm049137en
dc.relation.publisherversionhttps://doi.org/10.1242/dmm.049137
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLung canceren
dc.subjectSquamousen
dc.subjectNTCU-mouse modelen
dc.subjectSyngeneic cell linesen
dc.subjectRNASeqen
dc.subjectImmunotherapyen
dc.titleTwo cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinomaen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication610b3605-cc5a-426c-90d4-8920c27d230d
relation.isAuthorOfPublication.latestForDiscovery610b3605-cc5a-426c-90d4-8920c27d230d

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