GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogen Pseudomonas savastanoi pv. savastanoi

Lavado-Benito, Carla; Murillo Martínez, Jesús; Martínez Gil, Marta; Ramos, Cayo; Rodríguez Moreno, Luis

GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogen Pseudomonas savastanoi pv. savastanoi

dc.contributor.author	Lavado-Benito, Carla
dc.contributor.author	Murillo Martínez, Jesús
dc.contributor.author	Martínez Gil, Marta
dc.contributor.author	Ramos, Cayo
dc.contributor.author	Rodríguez Moreno, Luis
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2024-08-01T15:39:21Z
dc.date.available	2024-08-01T15:39:21Z
dc.date.issued	2024
dc.date.updated	2024-08-01T14:47:48Z
dc.description.abstract	GacS/GacA is a widely distributed two-component system playing an essential role as a key global regulator, although its characterization in phytopathogenic bacteria has been deeply biased, being intensively studied in pathogens of herbaceous plants but barely investigated in pathogens of woody hosts. P. savastanoi pv. savastanoi (Psv) is characterized by inducing tumours in the stem and branches of olive trees. In this work, the model strain Psv NCPPB 3335 and a mutant derivative with a complete deletion of gene gacA were subjected to RNA-Seq analyses in a minimum medium and a medium mimicking in planta conditions, accompanied by RT-qPCR analyses of selected genes and phenotypic assays. These experiments indicated that GacA participates in the regulation of at least 2152 genes in strain NCPPB 3335, representing 37.9 % of the annotated CDSs. GacA also controls the expression of diverse rsm genes, and modulates diverse phenotypes, including motility and resistance to oxidative stresses. As occurs with other P. syringae pathovars of herbaceous plants, GacA regulates the expression of the type III secretion system and cognate effectors. In addition, GacA also regulates the expression of WHOP genes, specifically encoded in P. syringe strains isolated from woody hosts, and genes for the biosynthesis of phytohormones. A gacA mutant of NCPPB 3335 showed increased virulence, producing large immature tumours with high bacterial populations, but showed a significantly reduced competitiveness in planta. Our results further extend the role of the global regulator GacA in the virulence and fitness of a P. syringae pathogen of woody hosts.	en
dc.description.sponsorship	This research was supported by project grants PID2020-115177RB-C21 and PID2020-115177RB-C22 financed by the Spanish Ministry of Science and Innovation (MCIN)/Agencia Estatal de Investigación (AEI)/10.13039/501100011033/ and by the European Regional Development Fund (ERDF) “A way to make Europe”. CL-B was supported by the FPI2018-084276 predoctoral grant. Open access was partially funded by grant QUAL21 012 IHSM, Consejería de Universidad, Investigación e Innovación, Junta de Andalucía.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.citation	Lavado-Benito, C., Murillo, J., Martínez-Gil, M., Ramos, C., Rodríguez-Moreno, L. (2024) GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogen Pseudomonas savastanoi pv. savastanoi. Frontiers in Plant Science, 15, 1-19. https://doi.org/10.3389/fpls.2024.1347982.
dc.identifier.doi	10.3389/fpls.2024.1347982
dc.identifier.issn	1664-462X
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/50598
dc.language.iso	eng
dc.publisher	Frontiers Media
dc.relation.ispartof	Frontiers in Plant Science, 15, 2024, 1347982
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115177RB-C21/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115177RB-C22/ES/
dc.relation.publisherversion	https://doi.org/10.3389/fpls.2024.1347982
dc.rights	© 2024 Lavado-Benito, Murillo, Martínez-Gil, Ramos and Rodríguez-Moreno. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Pseudomonas savastanoi	en
dc.subject	Pseudomonas syringae	en
dc.subject	GacS/GacA two component system	en
dc.subject	RNA-seq analysis	en
dc.subject	Gac-Rsm system	en
dc.subject	Woody host	en
dc.title	GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogen Pseudomonas savastanoi pv. savastanoi	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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