Leaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated CO2, temperature and low water availability

dc.contributor.authorAriz Arnedo, Idoia
dc.contributor.authorCruz, Cristina
dc.contributor.authorNeves, Tomé
dc.contributor.authorIrigoyen, Juan J.
dc.contributor.authorGarcía Olaverri, Carmen
dc.contributor.authorNogués, Salvador
dc.contributor.authorAparicio Tejo, Pedro María
dc.contributor.authorAranjuelo Michelena, Iker
dc.contributor.departmentEstatistika eta Ikerketa Operatiboaeu
dc.contributor.departmentNatura Ingurunearen Zientziakeu
dc.contributor.departmentEstadística e Investigación Operativaes_ES
dc.contributor.departmentCiencias del Medio Naturales_ES
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes
dc.date.accessioned2018-09-04T06:43:50Z
dc.date.available2018-09-04T06:43:50Z
dc.date.issued2015
dc.description.abstractThe natural 15N/14N isotope composition (δ15N) of a tissue is a consequence of its N source and N physiological mechanisms in response to the environment. It could potentially be used as a tracer of N metabolism in plants under changing environmental conditions, where primary N metabolism may be complex, and losses and gains of N fluctuate over time. In order to test the utility of δ15N as an indicator of plant N status in N2-fixing plants grown under various environmental conditions, alfalfa (Medicago sativa L.) plants were subjected to distinct conditions of [CO2] (400 vs. 700 μmol mol−1), temperature (ambient vs. ambient +4°C) and water availability (fully watered vs. water deficiency—WD). As expected, increased [CO2] and temperature stimulated photosynthetic rates and plant growth, whereas these parameters were negatively affected by WD. The determination of δ15N in leaves, stems, roots, and nodules showed that leaves were the most representative organs of the plant response to increased [CO2] and WD. Depletion of heavier N isotopes in plants grown under higher [CO2] and WD conditions reflected decreased transpiration rates, but could also be related to a higher N demand in leaves, as suggested by the decreased leaf N and total soluble protein (TSP) contents detected at 700 μmol mol−1 [CO2] and WD conditions. In summary, leaf δ15N provides relevant information integrating parameters which condition plant responsiveness (e.g., photosynthesis, TSP, N demand, and water transpiration) to environmental conditions.en
dc.description.sponsorshipThis work was supported by the Spanish Economy and Competiveness ministry (AGL-2012-37815-CO5-05, AGL2011-30386-C02-02 and Ramón y Cajal research grant) and by the Portuguese FCT (PTDC/BIA-ECS/122214/2010). IA was supported by a postdoctoral Fellowship from the Government of Navarra (Anabasid outgoing Programme) and by a postdoctoral Fellowship from the Portuguese FCT (SFRH/BPD/90436/2012).en
dc.format.extent10 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.3389/fpls.2015.00574
dc.identifier.issn1664-462X (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/30439
dc.language.isoengen
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Plant Science, 6: 574en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2012-37815-C05-05/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//AGL2011-30386-C02-01/ES/
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2015.00574
dc.rights© 2015 Ariz, Cruz, Neves, Irigoyen, Garcia-Olaverri, Nogués, Aparicio-Tejo and Aranjuelo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAlfalfaen
dc.subjectClimate changeen
dc.subjectGrowthen
dc.subjectdelta15Nen
dc.subjectPhysiologyen
dc.titleLeaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated CO2, temperature and low water availabilityen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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relation.isAuthorOfPublication5b37dbbe-41f2-4180-b1d7-9f64876eaa5b
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relation.isAuthorOfPublicationb8dd84ae-83ed-4e3f-873e-b0023505b3df
relation.isAuthorOfPublication.latestForDiscovery6f7563e2-1f07-4a25-8b38-4ac55bd18224

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