Leaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated CO2, temperature and low water availability

Ariz Arnedo, Idoia; Cruz, Cristina; Neves, Tomé; Irigoyen, Juan J.; García Olaverri, Carmen; Nogués, Salvador; Aparicio Tejo, Pedro María; Aranjuelo Michelena, Iker

Leaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated CO2, temperature and low water availability

dc.contributor.author	Ariz Arnedo, Idoia
dc.contributor.author	Cruz, Cristina
dc.contributor.author	Neves, Tomé
dc.contributor.author	Irigoyen, Juan J.
dc.contributor.author	García Olaverri, Carmen
dc.contributor.author	Nogués, Salvador
dc.contributor.author	Aparicio Tejo, Pedro María
dc.contributor.author	Aranjuelo Michelena, Iker
dc.contributor.department	Estatistika eta Ikerketa Operatiboa	eu
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.contributor.department	Estadística e Investigación Operativa	es_ES
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.date.accessioned	2018-09-04T06:43:50Z
dc.date.available	2018-09-04T06:43:50Z
dc.date.issued	2015
dc.description.abstract	The natural 15N/14N isotope composition (δ15N) of a tissue is a consequence of its N source and N physiological mechanisms in response to the environment. It could potentially be used as a tracer of N metabolism in plants under changing environmental conditions, where primary N metabolism may be complex, and losses and gains of N fluctuate over time. In order to test the utility of δ15N as an indicator of plant N status in N2-fixing plants grown under various environmental conditions, alfalfa (Medicago sativa L.) plants were subjected to distinct conditions of [CO2] (400 vs. 700 μmol mol−1), temperature (ambient vs. ambient +4°C) and water availability (fully watered vs. water deficiency—WD). As expected, increased [CO2] and temperature stimulated photosynthetic rates and plant growth, whereas these parameters were negatively affected by WD. The determination of δ15N in leaves, stems, roots, and nodules showed that leaves were the most representative organs of the plant response to increased [CO2] and WD. Depletion of heavier N isotopes in plants grown under higher [CO2] and WD conditions reflected decreased transpiration rates, but could also be related to a higher N demand in leaves, as suggested by the decreased leaf N and total soluble protein (TSP) contents detected at 700 μmol mol−1 [CO2] and WD conditions. In summary, leaf δ15N provides relevant information integrating parameters which condition plant responsiveness (e.g., photosynthesis, TSP, N demand, and water transpiration) to environmental conditions.	en
dc.description.sponsorship	This work was supported by the Spanish Economy and Competiveness ministry (AGL-2012-37815-CO5-05, AGL2011-30386-C02-02 and Ramón y Cajal research grant) and by the Portuguese FCT (PTDC/BIA-ECS/122214/2010). IA was supported by a postdoctoral Fellowship from the Government of Navarra (Anabasid outgoing Programme) and by a postdoctoral Fellowship from the Portuguese FCT (SFRH/BPD/90436/2012).	en
dc.format.extent	10 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.3389/fpls.2015.00574
dc.identifier.issn	1664-462X (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30439
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Plant Science, 6: 574	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2012-37815-C05-05/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-30386-C02-01/ES/
dc.relation.publisherversion	https://doi.org/10.3389/fpls.2015.00574
dc.rights	© 2015 Ariz, Cruz, Neves, Irigoyen, Garcia-Olaverri, Nogués, Aparicio-Tejo and Aranjuelo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Alfalfa	en
dc.subject	Climate change	en
dc.subject	Growth	en
dc.subject	delta15N	en
dc.subject	Physiology	en
dc.title	Leaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated CO2, temperature and low water availability	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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