In vitro evaluation of postbiotics produced from bacterial isolates obtained from rainbow trout and nile tilapia against the pathogens Yersinia ruckeri and Aeromonas salmonicida subsp. Salmonicida

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dc.contributor.authorQuintanilla Pineda, Mario Alejandro
dc.contributor.authorGarrote Achou, Chajira Camila
dc.contributor.authorDíaz, Jesús Vicente
dc.contributor.authorGutiérrez-Falcón, Ana
dc.contributor.authorBravo, María
dc.contributor.authorHerrera-Muñoz, Juan Ignacio
dc.contributor.authorPeña-Navarro, Nelson
dc.contributor.authorAlvarado, Carlos
dc.contributor.authorIbáñez Moya, Francisco C.
dc.contributor.authorMarzo Pérez, Florencio
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.contributor.departmentCienciases_ES
dc.contributor.departmentZientziakeu
dc.date.accessioned2023-08-10T13:58:41Z
dc.date.available2023-08-10T13:58:41Z
dc.date.issued2023
dc.date.updated2023-08-10T13:52:05Z
dc.description.abstractThe use of antibiotics in aquaculture leads to the proliferation of multidrug-resistant bacteria, and an urgent need for developing new alternatives to prevent and control disease has, thus, arisen. In this scenario, postbiotics represent a promising tool to achieve this purpose; thus, in this study, isolation and selection of bacteria to further produce and evaluate their postbiotics antibacterial activity against fish pathogens was executed. In this respect, bacterial isolates from rainbow trout and Nile tilapia were obtained and tested in vitro against Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida. From 369 obtained isolates, 69 were selected after initial evaluation. Afterwards, additional screening was carried out by spot-on-lawn assay to finally select twelve isolates; four were identified as Pediococcus acidilactici, seven as Weissella cibaria, and one as Weissella paramesenteroides by matrix assisted laser desorption/ionization, time-of-flight mass spectrometry (MALDI-TOF MS). Selected bacteria were used to obtain postbiotic products to test their antagonistic activity through coculture challenge and broth microdilution assays. The influence of incubation time prior to postbiotic production on antagonistic behavior was also recorded. Two isolates identified as W. cibaria were able to significantly reduce (p < 0.05) A. salmonicida subsp. salmonicida’s growth in the coculture challenge up to 4.49 ± 0.05 Log CFU/mL, and even though the reduction in Y. ruckeri was not as effective, some inhibition on the pathogen’s growth was reported; at the same time, most of the postbiotic products obtained showed more antibacterial activity when obtained from broth cultures incubated for 72 h. Based on the results obtained, the preliminary identification of the isolates that expressed the highest inhibitory activity was confirmed by partial sequencing as W. cibaria. Through our study, it can be concluded that postbiotics produced by these strains are useful to inhibit the growth of the pathogens and could, thereby, be applicable in further research to develop suitable tools as feed additives for disease control and prevention in aquaculture.en
dc.description.sponsorshipMaterial resources and facilities to perform the research were provided by PENTA S.L., with a contractual relationship between M.Q., and PENTA S.L. that has been funded by Gobierno de Navarra, by the aid for hiring industrial Ph.D. students: CE: 0011-1408-2020-000023, to fulfill the Ph.D agrifood program of Universidad Pública de Navarra.es_ES
dc.format.mimetypeapplication/pdfen
dc.identifier.citationQuintanilla-Pineda, M., Achou, C. G., Díaz, J., Gutiérrez-Falcon, A., Bravo, M., Herrera-Muñoz, J. I., Peña-Navarro, N., Alvarado, C., Ibañez, F. C., Marzo, F. (2023) In vitro evaluation of postbiotics produced from bacterial isolates obtained from rainbow trout and nile tilapia against the pathogens yersinia ruckeri and aeromonas salmonicida subsp. salmonicida. Foods, 12(4), 1-15. https://doi.org/10.3390/foods12040861.en
dc.identifier.doi10.3390/foods12040861es_ES
dc.identifier.issn2304-8158
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/45917
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofFoods 2023, 12(4), 861en
dc.relation.projectIDinfo:eu-repo/grantAgreement/Gobierno de Navarra//0011-1408-2020-000023/
dc.relation.publisherversionhttps://doi.org/10.3390/foods12040861
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAlternative treatment methodsen
dc.subjectAntibacterialen
dc.subjectBiological toolsen
dc.subjectDiseasesen
dc.subjectFurunculosises_ES
dc.subjectMicrobiotaes_Es
dc.subjectWeissellaes_ES
dc.subjectYersiniosises_ES
dc.titleIn vitro evaluation of postbiotics produced from bacterial isolates obtained from rainbow trout and nile tilapia against the pathogens Yersinia ruckeri and Aeromonas salmonicida subsp. Salmonicidaen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication1cd8b43c-65b9-450c-a993-ca4b4750d40f
relation.isAuthorOfPublicationae7c053b-14ad-49cb-828a-6bfac4bfa938
relation.isAuthorOfPublication2d886e9a-108d-4e3a-b091-7dc5f372eab5
relation.isAuthorOfPublication.latestForDiscoveryae7c053b-14ad-49cb-828a-6bfac4bfa938

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