The regulon of the RNA chaperone CspA and its auto-regulation in Staphylococcus aureus

Caballero Sánchez, Carlos; Menéndez Gil, Pilar; Catalán Moreno, Arancha; Vergara Irigaray, Marta; García Martínez, Begoña; Segura, Víctor; Irurzun Domínguez, Naiara; Villanueva San Martín, Maite; Ruiz de los Mozos Aliaga, Igor; Solano Goñi, Cristina; Lasa Uzcudun, Íñigo; Toledo Arana, Alejandro

The regulon of the RNA chaperone CspA and its auto-regulation in Staphylococcus aureus

dc.contributor.author	Caballero Sánchez, Carlos
dc.contributor.author	Menéndez Gil, Pilar
dc.contributor.author	Catalán Moreno, Arancha
dc.contributor.author	Vergara Irigaray, Marta
dc.contributor.author	García Martínez, Begoña
dc.contributor.author	Segura, Víctor
dc.contributor.author	Irurzun Domínguez, Naiara
dc.contributor.author	Villanueva San Martín, Maite
dc.contributor.author	Ruiz de los Mozos Aliaga, Igor
dc.contributor.author	Solano Goñi, Cristina
dc.contributor.author	Lasa Uzcudun, Íñigo
dc.contributor.author	Toledo Arana, Alejandro
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2018-09-06T12:20:12Z
dc.date.available	2018-09-06T12:20:12Z
dc.date.issued	2018
dc.description.abstract	RNA-binding proteins (RBPs) are essential to finetune gene expression. RBPs containing the coldshock domain are RNA chaperones that have been extensively studied. However, the RNA targets and specific functions for many of them remain elusive. Here, combining comparative proteomics and RBPimmunoprecipitation- microarray profiling, we have determined the regulon of the RNA chaperone CspA of Staphylococcus aureus. Functional analysis revealed that proteins involved in carbohydrate and ribonucleotide metabolism, stress response and virulence gene expression were affected by cspA deletion. Stress-associated phenotypes such as increased bacterial aggregation and diminished resistance to oxidative-stress stood out. Integration of the proteome and targetome showed that CspA posttranscriptionally modulates both positively and negatively the expression of its targets, denoting additional functions to the previously proposed translation enhancement. One of these repressed targets was its own mRNA, indicating the presence of a negative post-transcriptional feedback loop. CspA bound the 5 UTR of its own mRNA disrupting a hairpin, which was previously described as an RNase III target. Thus, deletion of the cspA 5 UTR abrogated mRNA processing and auto-regulation. We propose that CspA interacts through a U-rich motif, which is located at the RNase III cleavage site, portraying CspA as a putative RNase III-antagonist.	en
dc.description.sponsorship	European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme [646869]; Spanish Ministry of Economy and Competitiveness [BFU2011-23222, BIO2014-53530-R, BFU2014-56698-P]; Spanish National Research Council [CSIC-PII-201540I013]; C.J.C. was supported by predoctoral contract from the Public University of Navarre (UPNA), Spain. Funding for open access charge: European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme [646869].	en
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1093/nar/gkx1284
dc.identifier.issn	0305-1048 (Print)
dc.identifier.issn	1362-4962 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30527
dc.language.iso	eng	en
dc.publisher	Oxford University Press	en
dc.relation.ispartof	Nucleic Acids Research, 2018, Vol. 46, No. 3 1345–1361	en
dc.relation.projectID	info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/646869/
dc.relation.projectID	info:eu-repo/grantAgreement/ES/6PN/BFU2011-23222/
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//BIO2014-53530-R/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//BFU2014-56698-P/ES/
dc.relation.publisherversion	https://doi.org/10.1093/nar/gkx1284
dc.rights	© The Author(s) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc/4.0/
dc.subject	RNA chaperone CspA	en
dc.subject	Staphylococcus aureus	en
dc.title	The regulon of the RNA chaperone CspA and its auto-regulation in Staphylococcus aureus	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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