Characterization of the Amaranthus palmeri physiological response to glyphosate in susceptible and resistant populations

Fernández Escalada, Manuel; Gil Monreal, Miriam; Zabalza Aznárez, Ana; Royuela Hernando, Mercedes

Publication:
Characterization of the Amaranthus palmeri physiological response to glyphosate in susceptible and resistant populations

dc.contributor.author	Fernández Escalada, Manuel
dc.contributor.author	Gil Monreal, Miriam
dc.contributor.author	Zabalza Aznárez, Ana
dc.contributor.author	Royuela Hernando, Mercedes
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2017-04-11T16:22:15Z
dc.date.available	2017-04-11T16:22:15Z
dc.date.issued	2016
dc.description	Incluye 2 ficheros de datos	es_ES
dc.description.abstract	The herbicide glyphosate inhibits the plant enzyme 5-enolpyruvylshikimate3-phosphate synthase (EPSPS) in the aromatic amino acid (AAA) biosynthetic pathway. The physiologies of an Amaranthus palmeri population exhibiting resistance to glyphosate by EPSPS gene amplification (NC-R) and a susceptible population (NC-S) were compared. The EPSPS copy number of NC-R plants was 47.5-fold the copy number of NC-S plants. Although the amounts of EPSPS protein and activity were higher in NC-R plants than in NC-S plants, the AAA concentrations were similar. The increases in total free amino acid and in AAA contents induced by glyphosate were more evident in NC-S plants. In both populations, the EPSPS protein increased after glyphosate exposure, suggesting regulation of gene expression. EPSPS activity seems tightly controlled in vivo. Carbohydrate accumulation and a slight induction of ethanol fermentation were detected in both populations.	en
dc.description.sponsorship	This work was financially supported by a grant from the Ministerio Español de Economıa y Competitividad (AGL- 2013-40567R). M.F.-E. and M.G.-M. received funding from fellowships through the Universidad Publica de Navarra.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1021/acs.jafc.5b04916
dc.identifier.issn	0021-8561 (Print)
dc.identifier.issn	1520-5118 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/24071
dc.language.iso	eng	en
dc.publisher	ACS Publications	en
dc.relation.ispartof	Journal of Agricultural and Food Chemistry, 2016, 64 (1), pp 95–106	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2013-40567-R/ES/	en
dc.relation.publisherversion	https://dx.doi.org/10.1021/acs.jafc.5b04916
dc.rights	© 2015 American Chemical Society	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.subject	Carbohydrate accumulation	en
dc.subject	Ethanol fermentation	en
dc.subject	Free amino acid accumulation	en
dc.subject	Herbicide resistance	en
dc.subject	Physiological effects	en
dc.title	Characterization of the Amaranthus palmeri physiological response to glyphosate in susceptible and resistant populations	en
dc.type	info:eu-repo/semantics/article
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dspace.entity.type	Publication
relation.isAuthorOfPublication	e92ef4c3-70e2-4e1d-bc9a-b3e31397085f
relation.isAuthorOfPublication	b62dd25b-6298-44fe-8690-12267f5b3d70
relation.isAuthorOfPublication	0085d805-7945-48bf-994f-85bc2c88d92b
relation.isAuthorOfPublication	050bc121-21c9-467d-8bb6-611a789b1011
relation.isAuthorOfPublication.latestForDiscovery	e92ef4c3-70e2-4e1d-bc9a-b3e31397085f