Expression and localization of a Rhizobium-derived cambialistic superoxide dismutase in pea (Pisum sativum) nodules subjected to oxidative stress

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dc.contributor.authorAsensio, Aarón C.
dc.contributor.authorMarino Bilbao, Daniel
dc.contributor.authorJames, Euan K.
dc.contributor.authorAriz Arnedo, Idoia
dc.contributor.authorArrese-Igor Sánchez, César
dc.contributor.authorAparicio Tejo, Pedro María
dc.contributor.authorArredondo-Peter, Raúl
dc.contributor.authorMorán Juez, José Fernando
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.departmentCiencias del Medio Naturales_ES
dc.contributor.departmentNatura Ingurunearen Zientziakeu
dc.date.accessioned2025-01-30T08:59:15Z
dc.date.available2025-01-30T08:59:15Z
dc.date.issued2011-09-07
dc.date.updated2025-01-30T08:47:03Z
dc.description.abstractTwo phylogenetically unrelated superoxide dismutase (SOD) families, i.e., CuZnSOD (copper and zinc SOD) and FeMn-CamSOD (iron, manganese, or cambialistic SOD), eliminate superoxide radicals in different locations within the plant cell. CuZnSOD are located within the cytosol and plastids, while the second family of SOD, which are considered to be of bacterial origin, are usually located within organelles, such as mitochondria. We have used the reactive oxygen species¿producer methylviologen (MV) to study SOD isozymes in the indeterminate nodules on pea (Pisum sativum). MV caused severe effects on nodule physiology and structure and also resulted in an increase in SOD activity. Purification and N-terminal analysis identified CamSOD from the Rhizobium leguminosarum endosymbiont as one of the most active SOD in response to the oxidative stress. Fractionation of cell extracts and immunogold labeling confirmed that the CamSOD was present in both the bacteroids and the cytosol (including the nuclei, plastids, and mitochondria) of the N-fixing cells, and also within the uninfected cortical and interstitial cells. These findings, together with previous reports of the occurrence of FeSOD in determinate nodules, indicate that FeMnCamSOD have specific functions in legumes, some of which may be related to signaling between plant and bacterial symbionts, but the occurrence of one or more particular isozymes depends upon the nodule type.en
dc.description.sponsorshipThis work was supported by the Spanish MICIIN (grant numbers AGL2007-64432/AGR and AGL2010-16167).
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAsensio, A.C., Marino, D, James, E.K., Ariz, I., Arrese-Igor, C., Aparicio-Tejo, P.M., Arredondo-Peter, R., Moran, J.F. (2011) Expression and localization of a Rhizobium-derived cambialistic superoxide dismutase in pea (Pisum sativum) nodules subjected to oxidative stress. Molecular Plant-Microbe Interactions, 24(10), 1247-1257. https://doi.org/10.1094/MPMI-10-10-0253
dc.identifier.doi10.1094/MPMI-10-10-0253
dc.identifier.issn0894-0282
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/53171
dc.language.isoeng
dc.publisherThe American Phytopathological Society
dc.relation.ispartofMolecular Plant-Microbe Interactions, 24(10), 1247-1257
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//AGL2007-64432%2FAGR/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//AGL2010-16167/ES/
dc.relation.publisherversionhttps://doi.org/10.1094/MPMI-10-10-0253
dc.rights© 2011 The American Phytopathological Society.
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectSuperoxide dismutase (SOD)en
dc.subjectOxidative stressen
dc.subjectLegume nodulesen
dc.subjectRhizobium symbiosisen
dc.titleExpression and localization of a Rhizobium-derived cambialistic superoxide dismutase in pea (Pisum sativum) nodules subjected to oxidative stressen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery83664a8a-a37a-4ec9-916a-1b8621cc9402

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