The effectiveness of polyhydroxyalkanoate (PHA) extraction methods in gram-negative pseudomonas putida U

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dc.contributor.authorGetino, Luis
dc.contributor.authorGarcía, Irene
dc.contributor.authorCornejo Ibergallartu, Alfonso
dc.contributor.authorMateos, Raúl
dc.contributor.authorAriza-Carmona, Luisa M.
dc.contributor.authorSánchez-Castro, Natalia
dc.contributor.authorMorán Juez, José Fernando
dc.contributor.authorOlivera, Elías R.
dc.contributor.authorChamizo Ampudia, Alejandro
dc.contributor.departmentCienciases_ES
dc.contributor.departmentZientziakeu
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABen
dc.contributor.departmentInstitute for Advanced Materials and Mathematics - INAMAT2en
dc.date.accessioned2025-02-11T18:08:17Z
dc.date.available2025-02-11T18:08:17Z
dc.date.issued2025-01-09
dc.date.updated2025-02-11T17:52:56Z
dc.description.abstractBioplastics are emerging as a promising solution to reduce pollution caused by petroleum-based plastics. Among them, polyhydroxyalkanoates (PHAs) stand out as viable biotechnological alternatives, though their commercialization is limited by expensive downstream processes. Traditional PHA extraction methods often involve toxic solvents and high energy consumption, underscoring the need for more sustainable approaches. This study evaluated physical and chemical methods to extract PHAs from Pseudomonas putida U, a bacterium known to produce poly-3-hydroxyoctanoate P(3HO). Lyophilized cells underwent six extraction methods, including the use of the following: boiling, sonication, sodium hypochlorite (NaClO), sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), and chloroform. Physical methods such as boiling and sonication achieved yields of 70% and 60%, respectively, but P(3HO) recovery remained low (30–40%). NaClO extraction provided higher yields (80%) but resulted in significant impurities (70%). NaOH methods offered moderate yields (50–80%), with P(3HO) purities between 50% and 70%, depending on the conditions. Spectroscopic and analytical techniques (FTIR, TGA, NMR, GPC) identified 0.05 M NaOH at 60 °C as the optimal extraction condition, delivering high P(3HO) purity while minimizing environmental impact. This positions NaOH as a sustainable alternative to traditional halogenated solvents, paving the way for more eco-friendly PHA production processes.en
dc.description.sponsorshipThe authors want to express their gratitude to the 'Programa de Bachillerato de Investigación/Excelencia (BIE)' of the Junta de Castilla y León in Spain and the project TED2021-132593B-I00, funded by MCIN/AEI/10.13039/501100011033 and by the European Union 'NextGeneration EU'/PRTR. On the other hand, to the Ministerio de Universidades, Real Decreto 1059/2021, de 30 de noviembre, por el que se regula la concesión directa de diversas subvenciones a las universidades participantes en el proyecto 'Universidades Europeas de la Comisión Europea' y 'European Education and Culture Executive Agency', Project: 101124439--EURECA-PRO 2.0--ERASMUS-EDU-2023-EUR-UNIV.
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGetino, L., García, I., Cornejo, A., Mateos, R., Ariza-Carmona, L. M., Sánchez-Castro, N., Moran, J. F., Olivera, E. R., Chamizo-Ampudia, A. (2025) The effectiveness of polyhydroxyalkanoate (PHA) extraction methods in gram-negative pseudomonas putida U. Polymers, 17(2), 1-18. https://doi.org/10.3390/polym17020150.
dc.identifier.doi10.3390/polym17020150
dc.identifier.issn2073-4360
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/53344
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofPolymers 2025, 17(2), 150
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI//TED2021-132593B-I00/
dc.relation.publisherversionhttps://doi.org/10.3390/polym17020150
dc.rights© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPseudomonas putida Uen
dc.subjectExtraction processesen
dc.subjectPolyhydroxyalkanoatesen
dc.subjectEnvironmentally friendly solventsen
dc.subjectPurificationen
dc.titleThe effectiveness of polyhydroxyalkanoate (PHA) extraction methods in gram-negative pseudomonas putida Uen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublicationeabef884-715a-460f-b2a1-e62568297b4a
relation.isAuthorOfPublicationcad13109-2660-4ec4-80c8-e4bcc0685382
relation.isAuthorOfPublicationc2f4abac-9941-4b6c-ac17-120d94b317cc
relation.isAuthorOfPublication.latestForDiscoveryeabef884-715a-460f-b2a1-e62568297b4a

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