Study of the bacillus thuringiensis Cry1Ia protein oligomerization promoted by midgut brush border membrane vesicles of lepidopteran and coleopteran insects, or cultured insect cells

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dc.contributor.authorKhorramnejad, Ayda
dc.contributor.authorDomínguez Arrizabalaga, Mikel
dc.contributor.authorCaballero Murillo, Primitivo
dc.contributor.authorEscriche, Baltasar
dc.contributor.authorBel, Yolanda
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2020-07-06T08:41:23Z
dc.date.available2020-07-06T08:41:23Z
dc.date.issued2020
dc.description.abstractBacillus thuringiensis (Bt) produces insecticidal proteins that are either secreted during the vegetative growth phase or accumulated in the crystal inclusions (Cry proteins) in the stationary phase. Cry1I proteins share the three domain (3D) structure typical of crystal proteins but are secreted to the media early in the stationary growth phase. In the generally accepted mode of action of 3D Cry proteins (sequential binding model), the formation of an oligomer (tetramer) has been described as a major step, necessary for pore formation and subsequent toxicity. To know if this could be extended to Cry1I proteins, the formation of Cry1Ia oligomers was studied by Western blot, after the incubation of trypsin activated Cry1Ia with insect brush border membrane vesicles (BBMV) or insect cultured cells, using Cry1Ab as control. Our results showed that Cry1Ia oligomers were observed only after incubation with susceptible coleopteran BBMV, but not following incubation with susceptible lepidopteran BBMV or non-susceptible Sf21 insect cells, while Cry1Ab oligomers were persistently detected after incubation with all insect tissues tested, regardless of its host susceptibility. The data suggested oligomerization may not necessarily be a requirement for the toxicity of Cry1I proteins.en
dc.description.sponsorshipThis work was supported by grants from the Spanish Ministry of Science, Innovation and Universities, the State Research Agency of Spain and the European FEDER founds (Refs. AGL2015-70584-C2 and RTI2018-095204-B-C21), and by the Generalitat Valenciana (GVPROMETEOII-2015-001). M. Domínguez received a predoctoral fellowship from the Universidad Pública de Navarra, Spain.en
dc.format.extent15 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.3390/toxins12020133
dc.identifier.issn2072-6651
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/37340
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofToxins, 2020, 12(2), 133en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2015-70584-C2-2-R/ES/
dc.relation.publisherversionhttps://doi.org/10.3390/toxins12020133
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCry1Aben
dc.subjectOligomer formationen
dc.subjectSf21 cell lineen
dc.subjectOstrinia nubilalisen
dc.subjectLobesia botranaen
dc.subjectLeptinotarsa decemlineataen
dc.subjectBioassayen
dc.titleStudy of the bacillus thuringiensis Cry1Ia protein oligomerization promoted by midgut brush border membrane vesicles of lepidopteran and coleopteran insects, or cultured insect cellsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication7e933f4e-d0bf-4bc7-9d41-13c08c72800d
relation.isAuthorOfPublicationecde8e03-14c2-46a3-9e89-ae3b2c668297
relation.isAuthorOfPublication.latestForDiscovery7e933f4e-d0bf-4bc7-9d41-13c08c72800d

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